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A method for increasing sugar content in strawberry fruit

A sugar content, strawberry technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of poor flavor quality, large gap in fruit flavor quality, and strawberry fruit sugar content Low-level problems, to achieve the effect of increasing sucrose content and improving taste

Active Publication Date: 2022-05-17
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to different varieties of strawberries, the quality of fruit flavor varies greatly.
The cultivated area and market share of varieties bred by conventional hybridization methods in my country are lower than those of Japanese and American varieties. One of the most important reasons is that the low sugar content of strawberry fruit leads to poor flavor quality.

Method used

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  • A method for increasing sugar content in strawberry fruit
  • A method for increasing sugar content in strawberry fruit
  • A method for increasing sugar content in strawberry fruit

Examples

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Embodiment 1

[0045] (1) Method for Screening Strawberry Polyol / Monosaccharide Transporter Genes Based on cDNA Sequence 1. Strawberry Polyol / Monosaccharide Transporter Gene Mining. The whole strawberry genome (Fragaria vesca) published on the NCBI and Strawberry Genome website (http: / / www.strawberrygenome.org) uses the protein sequence of the Arabidopsis monosaccharide transporter PMT (polyol / monosaccharidetransporter) as a template, respectively for Strawberry The genomes were compared, and a total of 13 Arabidopsis AtPMT homologous suspected sugar transporter genes were obtained.

[0046] 2. Primer design. Using software Premier primers5.0, quantitative PCR primers were designed in the cDNA region of each sugar transporter gene, and 18SrRNA was used as an internal reference gene for fluorescent quantitative PCR analysis. Quantitative PCR primers (Table 1) were designed according to the cDNA sequence of the gene obtained above, and 18SrRNA was used as an internal reference gene.

[0047]...

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Abstract

The invention provides a method for increasing the sugar content in strawberry fruit. The gene encoding strawberry polyol / monosaccharide transporter is introduced into the target strawberry fruit for transient overexpression to obtain strawberry fruit with high sugar content; The gene of the monosaccharide transporter consists of the base sequence shown in Sequence 1 in the sequence table. The method of the invention increases the sugar content in the strawberry fruit compared with the control, greatly increases the fructose content in the strawberry fruit, and effectively improves the strawberry fruit. Fruity taste.

Description

technical field [0001] The invention relates to the field of strawberry planting, in particular to a method for increasing the sugar content in strawberry fruit. Background technique [0002] Strawberry is a perennial herb of the family Rosaceae. The fruit is pointed and ovoid, delicious, red and tender, with juicy pulp. Because of its rich vitamin C and wide application value, it is known as the "Queen of Fruits". However, due to different varieties of strawberries, the quality of fruit flavor varies greatly. The cultivated area and market share of varieties bred by conventional hybridization methods in my country are lower than those of Japanese and American varieties. One of the most important reasons is that the low sugar content of strawberry fruit leads to poor flavor quality. How to increase the sugar content of strawberry fruit, improve the flavor quality of fruit, and increase market share have become technical problems demanding prompt solution. [0003] Sugar i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/08A01H6/74
CPCC12N15/8245C07K14/415
Inventor 伊兴凯魏书
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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