HIV-1 total dna quantitative detection primer pair, probe and detection kit that can cover multiple subtypes

Abstract

The invention discloses a HIV-1 total DNA quantitative detection primer pair and probe that can cover multiple subtypes, wherein, the upstream primer HIV-U1 sequence of the primer pair is shown in SEQ ID No.1; the downstream primer HIV The sequence of -R1 is shown in SEQ ID No.2; the sequence of the probe Probe-HIV is shown in SEQ ID No.3. The invention uses degenerate primers to detect HIV DNA with high sensitivity and specificity, can successfully detect 12 common subtypes and rare HIV subtypes, and has wider subtype detection coverage than other current commercial detection kits. The invention can be used for the quantification of the total HIV DNA of HIV epidemic strains in my country at present. The method is simple to operate, and can be completed from sample DNA extraction to real-time fluorescent quantitative PCR amplification in one day, and the price is relatively low. It can be used to monitor the persistent infection status of HIV-infected individuals in large cohorts, evaluate the effect of cART treatment, and can also be used for Auxiliary diagnosis of early HIV infection and diagnosis of infection in newborns of HIV-positive mothers.

Description

technical field [0001] The invention relates to a HIV-1 total DNA quantitative detection primer pair, a probe and a detection kit, specifically a highly sensitive and specific HIV-1 total DNA quantitative detection primer pair that can cover multiple subtypes, Probes and detection kits. Background technique [0002] As of the end of May 2018, my country reported 810,000 living HIV / AIDS patients and 250,000 reported deaths. There are still 30% of infected people who are not aware of the infection and have not been discovered. The epidemic situation is not optimistic. Therefore, early detection of HIV-infected persons, early treatment, reducing the viral load of infected persons, and controlling the source of infection from the source are an important strategic measure for the prevention and control of HIV infection. [0003] After HIV infection, the first viral markers to appear are viral nucleic acids and antigens. After the HIV virus enters the human body, it first infect...

AI Technical Summary

Problems solved by technology

Due to the large number of amplification primer pairs, the biggest technical problems are cumbersome operation, high cost, amplification contamination and inaccuracy and other practical application problems, and the lack of clinical data on genotype coverage

In addition, the increasing diversity of HIV genotypes in China has also brought a lot of uncertainty to the detection efficiency of a single kit

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