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A stem cell scaffold applied to iron overload area and preparation method thereof

A stem cell and iron overload technology, applied in the field of stem cell scaffolds, can solve the problems of reduced tissue repair function, more cell death, less survival, etc., and achieves the effect of eliminating iron overload, improving cell growth, and good effect.

Active Publication Date: 2020-07-24
海默斯(重庆)医学生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have shown that iron overload can affect the proliferation and differentiation of stem cells. Therefore, implanting stem cells directly into the iron-overloaded environment of hemorrhagic disease lesions will greatly reduce their tissue repair function
However, in the prior art, the stem cell scaffolds used in iron overloaded areas are mostly single-layer structures, with more cell death and less survival.

Method used

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  • A stem cell scaffold applied to iron overload area and preparation method thereof
  • A stem cell scaffold applied to iron overload area and preparation method thereof
  • A stem cell scaffold applied to iron overload area and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation process of the bilayer keratin gel cell scaffold is as follows:

[0029] The keratin aqueous solution is prepared at room temperature, the water used is distilled water, the keratin concentration of the inner layer is 30%, magnetic stirring is performed at 37°C, the stirring speed is 200-300rpm, and the time is 5.5-7h to make it completely mixed. The concentration of keratin in the outer layer is 35%, the concentration of minocycline is 3%, and the magnetic stirring is performed at 37° C. at 250 rpm for 6 hours to make it completely mixed.

[0030] Rat bone marrow mesenchymal stem cells were collected for primary culture. Cells were collected at P3, mixed with the inner gel, and then the inner and outer gels were placed in syringes in a ratio of 1:2, and slowly injected into a 48-well plate cultured in an iron overload environment for 24 hours, and then stained for life and death.

[0031] Comparative example: prepare keratin aqueous solution at room te...

Embodiment 2

[0033] The preparation process of the bilayer keratin gel cell scaffold is as follows:

[0034] The keratin aqueous solution was prepared at room temperature, the water used was distilled water, the keratin concentration of the inner layer was 25%, magnetic stirring was performed at 37°C, the stirring speed was 200rpm, and the stirring time was 5.5h to make it completely mixed. The concentration of keratin in the outer layer is 30%, the concentration of minocycline is 3%, magnetic stirring is carried out at 37°C, the stirring speed is 200rpm, and the stirring time is 5.5h to make it completely mixed.

[0035] Rat bone marrow mesenchymal stem cells were collected for primary culture. Cells were collected at P3, mixed with the inner gel, and then the inner and outer gels were placed in syringes in a ratio of 1:2, and slowly injected into a 48-well plate cultured in an iron overload environment for 24 hours, and then stained for life and death.

Embodiment 3

[0037] The preparation process of the bilayer keratin gel cell scaffold is as follows:

[0038] The keratin aqueous solution was prepared at room temperature, the water used was distilled water, the keratin concentration of the inner layer was 35%, magnetic stirring was carried out at 37°C, the stirring speed was 300rpm, and the stirring time was 7h to make it completely mixed. The concentration of keratin in the outer layer is 40%, the concentration of minocycline is 3%, magnetic stirring is performed at 37°C, the stirring speed is 300rpm, and the stirring time is 7h to make it completely mixed.

[0039] Rat bone marrow mesenchymal stem cells were collected for primary culture, and the cells were collected at P3, mixed with the inner gel, and then the inner and outer gels were placed in syringes at a ratio of 1:2, and slowly injected into a 48-well plate cultured in an iron overload environment for 24 hours, and then stained for life and death.

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Abstract

The invention discloses a stem cell scaffold applied to an iron overload region. The stem cell scaffold is a double-layered keratin gel cell having a concentric structure; the stem cell scaffold is composed a low-molecular weight keratin hydrogel outer layer coated with deferoxamine and a high-molecular weight keratin hydrogel inner layer wrapped with stem cells and trophic factors; and the stem cell scaffold is the double-layer concentric structure, the inner layer provides cell growth, and the outer layer eliminates the unfavorable environment, so cell growth is improved, the survival rate is increased, and the stem cell scaffold achieves a higher cell survival rate and a better effect than traditional transplanted cells after cerebral hemorrhage. The double-layered keratin gel cell scaffold is injected into the cerebral hemorrhage site in order to rapidly adsorb residual overload iron ions surrounding hematoma tissues by the adsorption effect of gel and release embedded drugs for chelating the iron ions, so the iron overload is eliminated; and the transplanted stem cells provide a neuroprotection effect, and can be differentiated into neurons and glial cells to repair damaged parts.

Description

technical field [0001] The invention relates to a stem cell scaffold, in particular to a stem cell scaffold applied to an iron overload area and a preparation method thereof. Background technique [0002] Iron is an indispensable trace element for the human body. It is an important component of hemoglobin, myoglobin and various enzymes. However, the abnormal metabolism and massive accumulation of iron in the human body will lead to local iron overload in the tissue. The harm of iron overload is mainly concentrated in the excessive iron catalyzing the production of active oxygen free radicals, leading to tissue oxidation and cytotoxic damage. Clinically, iron overload can be caused by primary iron overload caused by HFE gene mutation, or secondary iron overload caused by some hemorrhagic diseases including cerebral hemorrhage, multiple blood transfusions, excessive intake of iron, etc. . Stem cells are a kind of cell population with self-renewal and differentiation potentia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/38A61L27/52A61L27/54A61L27/58
CPCA61L27/227A61L27/3834A61L27/52A61L27/54A61L27/58A61L2300/204A61L2300/40A61L2430/32C08L89/00
Inventor 郝石磊屈清王伯初
Owner 海默斯(重庆)医学生物技术有限公司
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