A device for swine visceral tissue biopsy and nucleic acid extraction

Abstract

The invention discloses a device for sampling pig visceral tissue and extracting nucleic acid. The needle for living body sampling includes a needle body acting on tissues in the body and a support body for supporting the needle body. The sampling needle and the outer cutting needle, the length of the inner sampling needle is greater than the length of the outer cutting needle, the inner sampling needle passes through the inner cavity of the outer cutting needle, the two can move relatively, and the inner sampling needle is provided with a plurality of tines along the radial direction at the tip , the tip of the outer cutting needle has a cutting edge; the extraction tube includes a reaction tube with a sealing cover at both ends and an auxiliary sleeve fixed on the outer periphery of the reaction tube, and the auxiliary sleeve is provided with a handle near the right end of the reaction tube ; After the sealing cover at the left end of the reaction tube is opened, it is transitionally matched with an outer trocar needle for puncturing the body surface, and the outer cutting needle passes through the inner cavity of the outer trocar needle, and the two can move relatively; the kit includes a freeze-dried powder form nucleic acid release agent. The invention can be used for living body sampling and nucleic acid extraction.

Description

Technical field: [0001] The invention relates to a device for performing invasive operations on animal tissues to obtain tissue samples and extract nucleic acids from the samples, in particular to a device for sampling pig visceral tissues and extracting nucleic acids. Background technique: [0002] Animal diseases have an important impact on animal husbandry and human food safety, such as the highly pathogenic porcine blue-ear disease that has been prevalent in pigs in recent years, which is caused by porcine reproductive and respiratory syndrome (commonly known as blue-ear disease). An acute viral infectious disease caused by virus variants, characterized by reproductive disorders in sows and respiratory diseases in piglets and fattening pigs. Pigs of different breeds, ages and sexes are susceptible. The main clinical features are abortion, stillbirth and mummified fetuses , weak fetus, dyspnea and other symptoms. Highly pathogenic porcine blue-ear disease is one of the m...

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned virus detection technology can give a good indication of the state of disease infection, but these operations often require professionally trained experimenters, and the cost is high and the time required is long, especially for small and medium farms (households) It is difficult to realize autonomous detection

Images