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Method for producing paper-based microfluidic chip based on controllability of inkjet printing technology and used for quantitative detection of glucose

A microfluidic chip and quantitative detection technology, which is applied in the field of detection of glucose in human serum, can solve the problems of time-consuming, costly, and large sample consumption

Inactive Publication Date: 2019-01-01
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of biomarkers in serum usually consumes a lot of time and cost, and consumes a large amount of samples. Therefore, establishing an accurate, fast and sensitive blood glucose detection method is very important for clinical medical diagnosis and research. significance

Method used

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  • Method for producing paper-based microfluidic chip based on controllability of inkjet printing technology and used for quantitative detection of glucose
  • Method for producing paper-based microfluidic chip based on controllability of inkjet printing technology and used for quantitative detection of glucose
  • Method for producing paper-based microfluidic chip based on controllability of inkjet printing technology and used for quantitative detection of glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The research steps of monochrome printing rule of inkjet printer are as follows:

[0040] When printing, the printer can have color printing (regulated by opacity) and black and white printing (also known as grayscale printing, regulated by grayscale value) in color matching.

[0041] (1) Research on monochrome printing rules—color printing mode

[0042] Using drawing software Adobe Illustrator CS6, the design area size is 0.25 mm 2 , the border is set to none, and the inside is filled with four colors of black, cyan, yellow, and magenta, and the colors are set to 10%, 20%, 30%, 40%, 50%, and 100% in order of opacity. In order to facilitate visual observation, the average nesting area outside the small square is 9 mm 2 only the perimeter black frame of the square. Then use the printer to print on ordinary A4 printing paper with the printing quality of the standard mode, place it under a microscope for observation, and use a smart phone to take pictures and record it....

Embodiment 2

[0047] The research steps of the two-color printing rules of inkjet printers are as follows:

[0048] Since black printing has only one color, it is not included in the scope of the study of the two color rules. Here, only the color rule under the control of opacity is studied. Using drawing software Adobe Illustrator CS6, the design area size is 0.25 mm 2 A small square with a border set to none. Because the colors in printing are formulated in CMYK mode, that is, the four colors of cyan, magenta, yellow, and black are mixed in different proportions, so the research on the two-color rule is based on the use of software to control two of the colors to be none. , to fix one of the colors and continuously increase the opacity of the other color. And because black is actually produced by mixing and superimposing ink dots of various colors under the control of opacity, the ink dots are messy, and it is difficult to summarize the rules, so the opacity of black is set to 0, which...

Embodiment 3

[0052] Research and summary of multi-color printing rules of inkjet printers:

[0053] Calculate the number of ink dots of the matching color in the two-color rule, combined with the number of ink dots with specific color opacity obtained in the two-color rule and the number of ink dots with specific color opacity in the single-color rule, the results are summarized in Table 1 And the results of the color law study shown in Table 2. As shown in Table 1, taking cyan as an example, when studying the two-color law, when the opacity of cyan is fixed, the number of cyan ink dots remains constant, and the number of cyan ink dots is the same as the number of cyan ink dots under the specific opacity of cyan in the study of single-color law. The number of ink dots remains the same. As shown in Table 2, taking cyan as an example, when studying the two-color rule, when the opacity of cyan is fixed, the ratio of cyan to magenta or yellow under any opacity, and the number of cyan ink dots...

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Abstract

The invention discloses a method for producing a paper-based microfluidic chip based on the controllability of an inkjet printing technology and used for quantitative detection of glucose. Since the inkjet printing technology has the characteristics of capacity of precisely controlling ink droplet volumes, high repeatability, flexibility in pattern design and the like, a paper-based microfluidic chip technology is easy to process and is minisized and portable, and glucose oxidase has the characteristic of capacity of specifically catalyzing the reaction of glucose, a GOx-HRP-TMB-H2O2 double-enzyme color development system is controllably integrated on a paper chip via an inkjet printer for a reaction through the combined usage of the inkjet printing technology, the paper-based microfluidicchip technology and the glucose oxidase. After color development, the inkjet printer is used for scanning imaging, and then grayscale analysis and data processing are performed by software such as Adobe Photoshop CS6 and Image J 2X; so a novel, fast, accurate and highly-sensitive method for quantitative detection of glucose is established and is applied to quantitative determination of glucose inthe human serum. The method of the invention has important practical significance to clinical medical diagnosis, treatment evaluation, prognosis judgment and the like of diabetes.

Description

technical field [0001] The invention relates to a method for manufacturing a paper-based microfluidic chip for quantitative detection of glucose based on the controllability of inkjet printing technology, belonging to the technical field of detection. The paper chip can be used for the detection of glucose in human serum. Background technique [0002] In the 1980s, due to the development of micro-processing technology, micro-total analysis system "μTAS" (also known as "Lab-on-chip") came into being under the trend of miniaturization. The miniaturization of analysis systems has brought about significantly reduced sample volume and lower reagent consumption, and microfluidic chips are one of them. The so-called microfluidic chip is a kind of microfluidic functional unit based on micro-electromechanical processing technology, using micro-channels as a network to connect micro-pumps, micro-valves, micro-detection elements, etc., through the manipulation and control of micro-flui...

Claims

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Application Information

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IPC IPC(8): G01N33/66B01L3/00
CPCB01L2200/10B01L2300/0861B01L2300/126G01M3/02G01N33/66
Inventor 刘爱林连芯刘萌萌雷云彭花萍陈伟林新华
Owner FUJIAN MEDICAL UNIV
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