Immobilized sulfate reducing bacterium particle preparation method and application

A sulfate and granule technology, applied in biochemical equipment and methods, chemical instruments and methods, and microbial-based methods, can solve problems such as slow reaction, high energy consumption, and poor effluent quality, so as to promote growth and activity, Good settling performance, accelerated removal effect

Inactive Publication Date: 2019-01-04
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complex composition of heavy metal wastewater, large concentration differences, and strict treatment standards, there are some technical problems in traditional treatment technologies: large amount of treatment agent, high energy consumption, difficult to control reaction, slow reaction, unsatisfactory effect, and effluent quality. Poor, unstable residue, secondary pollution and difficult recovery of precious metals, etc.
However, the traditional microbial immobilization technology needs to add polymer carriers and cross-linking agents, and the stability is poor after long-term operation.

Method used

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  • Immobilized sulfate reducing bacterium particle preparation method and application
  • Immobilized sulfate reducing bacterium particle preparation method and application
  • Immobilized sulfate reducing bacterium particle preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, such as figure 1 As shown, it includes the following steps in turn:

[0030] S1) Add 0.2ml of graphene oxide sol into 50ml of sulfate-reducing bacteria culture medium, that is, the volume ratio of the two is 0.4%, and stir and mix;

[0031] S2) The pH value of the culture medium is adjusted to 7.5, and 0.5ml of sulfate-reducing bacteria bacterial liquid (concentration is 2.1×10) is inoculated into the sulfate-reducing bacteria medium. 10 cfu / ml), the volume ratio of sulfate-reducing bacteria bacterium liquid and sulfate-reducing bacteria culture medium is 1%, stirring and mixing;

[0032] S3) Infuse nitrogen gas for 20 minutes to ensure an anaerobic culture environment. After sealing, culture at 30° C. for 18 hours and enter a stable period. The obtained black particles are BY-rGO particles.

Embodiment 2

[0034] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, and it is similar to embodiment 1, and difference is: in S1) 0.4ml graphene oxide sol is added in the sulfate-reducing bacteria culture medium of 50ml, promptly two The volume ratio of the latter is 0.8%.

Embodiment 3

[0036] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, and it is similar to embodiment 1, and difference is: in S1) 0.6ml graphene oxide sol is added in the sulfate-reducing bacteria culture medium of 50ml, promptly two The volume ratio of the latter was 1.2%.

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Abstract

The invention belongs to the technical field of wastewater biological treatment and discloses an immobilized sulfate reducing bacterium particle preparation method and application. The preparation method includes steps: adding graphene oxide sol into a sulfate reducing bacterium culture medium, stirring and mixing; adding sulfate reducing bacteria liquid into the sulfate reducing bacterium culturemedium, stirring and mixing; culturing for 18-72h at 10-45 DEG C in a pH value of 2-12 under anaerobic conditions to obtain particles namely immobilized sulfate reducing bacteria particles. Accordingto the preparation method, reduced graphene oxide serves as a biological carrier for immobilizing sulfate reducing bacteria, activity of the sulfate reducing bacteria is improved, and influences of heavy metals on the sulfate reducing bacteria are weakened; in addition, the preparation method is simple and convenient, high stability of the immobilized sulfate reducing bacteria in products is achieved, and extensive popularization and utilization can be realized.

Description

technical field [0001] The invention belongs to the technical field of wastewater biological treatment, and in particular relates to a preparation method and application of immobilized sulfate-reducing bacteria particles. Background technique [0002] Sulfate widely exists in domestic sewage, industrial wastewater and agricultural wastewater, and its concentration range is wide; if it is not treated properly, it will cause serious pollution to the environment. Symptoms of sulfate poisoning in humans include indigestion and diarrhea. In addition, industrial sulfate wastewater (such as mine wastewater, desulfurization wastewater, etc.) often contains copper, nickel, chromium, lead, gold, silver, mercury and other heavy metal ions or their complex ions. Due to the chemical stability of such heavy metal ions in the natural environment, they can accumulate in organisms and have chronic toxicity; improper handling will have a serious impact on agriculture, fisheries, etc., and en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/20C02F3/34C02F101/10C12R1/01
CPCC02F3/345C02F2101/101C12N1/20C12N11/14
Inventor 阎佳张鸿郭王思霁钟铿锵肖唐付
Owner GUANGZHOU UNIVERSITY
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