Screening method of chicken breeding materials

A screening method and material technology, which is applied in the field of chicken breeding material screening, can solve problems such as chicken breeding materials that have not yet appeared, and achieve the effects of reducing generation interval time, simple and fast operation, and reducing invalid experiments

Active Publication Date: 2019-01-11
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are no related reports on the screening of chicken breeding materials using microsatellite markers combined with mtDNA D-loop sequence analysis

Method used

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  • Screening method of chicken breeding materials
  • Screening method of chicken breeding materials
  • Screening method of chicken breeding materials

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Experimental program
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Embodiment 1

[0052] Example 1: Selection of Guifeng No. 2 Yellow Chicken Breeding Materials

[0053] The screening method of Guifeng No. 2 yellow chicken breeding material of the present embodiment comprises the following steps:

[0054] Step 1: Extraction of Genomic DNA

[0055] The samples come from 60 Nanning Sanhuang Chickens (NN), 60 Yulin Xingye Sanhuang Chickens (XY), 60 Beiliurong County Sanhuang Chickens (RX), 60 Yulin Bobai Sanhuang Chickens (BB) and Hezhou Xindu Sanhuang Chickens (XD) from Guangxi Sanhuang Chickens. 60, 60 Cenxi classical three yellow chicken (GD) and other 6 strains, a total of 360 samples.

[0056] Using a DNA kit to extract the genomic DNA in the blood of the above-mentioned sample to be tested.

[0057] Step 2: Microsatellite markers

[0058] Select microsatellite numbers as MCW0123, ADL0112, MCW0014, MCW0034, MCW0103, MCW0295, MCW0078, MCW0222, MCW0098, MCW01111, MCW0037, MCW0248, LEI0166, ADL0268, MCW0216, MCW0020, MCW0186, PCR-fluorescent labeling-sem...

Embodiment 2

[0082]Embodiment 2: Selection of Jinling chicken breeding material

[0083] The screening method of the Jinling chicken breeding material of the present embodiment comprises the following steps:

[0084] Step 1: Extraction of Genomic DNA

[0085] The samples come from 60 Guangxi Sanhuang chickens (TY), 60 Xiayan chickens (XY), 60 Ma chickens (MA), 60 Nandan Yao chickens (ND), 60 Guangxi black-bone chickens (GX), and Longsheng Phoenix chickens ( LS) 60 samples, Avian chicken (AA) 50, Roman chicken (RM) 50, a total of 460 samples of 8 breeds.

[0086] Using a DNA kit to extract the genomic DNA in the blood of the above-mentioned sample to be tested.

[0087] Step 2: Microsatellite markers

[0088] Select microsatellite numbers as MCW0123, ADL0112, MCW0014, MCW0034, MCW0103, MCW0295, MCW0078, MCW0222, MCW0098, MCW01111, MCW0037, MCW0248, LEI0166, ADL0268, MCW0216, MCW0020, MCW0186, PCR-fluorescent labeling-semi-automatic genome scanning to obtain a genome scanning map.

[00...

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Abstract

The invention discloses a screening method of chicken breeding materials, belonging to the field of agricultural livestock and poultry breeding. The screening method of chicken breeding materials comprises the following steps: 1) extracting genomic DNA; 2) performing microsatellite mark and PCR reaction; 3) performing mtDNA PCR reaction and sequencing; 4) performing statistical analysis; and 5) selecting breeding material. The invention adopts molecular biotechnology to analyze genetic diversity and genetic distance of breeding material, selects individuals with rich genetic diversity as breeding material, ensures rich genetic information, reduces blindness and risk of breeding, reduces breeding time, improves breeding efficiency and reduces breeding cost.

Description

technical field [0001] The invention relates to a method for screening chicken breeding materials, belonging to the field of agricultural livestock and poultry breeding. Background technique [0002] The screening of livestock and poultry breeding materials in the prior art is often carried out based on information such as body shape appearance, pedigree and production performance measurement. The above method is mainly based on the phenotypic value of livestock and poultry to evaluate the breeding materials of livestock and poultry, which cannot truly and comprehensively reflect the characteristics of livestock and poultry species, has certain limitations and unpredictability, and the evaluation time is long and the cost is high. [0003] In recent years, with the development of molecular biology techniques, researchers have evaluated livestock and poultry breed resources by constructing molecular genetic maps of livestock and poultry breeds from the genetic level. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858G16B40/00G16B20/20
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2531/113C12Q2537/165C12Q2535/122
Inventor 廖玉英莫国东黄英飞孙俊丽韦凤英
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
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