Artificial planting method of glycosmis citrifolia
A planting method and artificial technology, applied in botany equipment and methods, fertilization methods, horticultural methods, etc., can solve the problems of artificial planting that have not been reported, achieve medicinal value retention, resource utilization, and strong adaptability Effect
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Embodiment 1
[0029] A kind of artificial planting method of Shanxiaoju comprises the steps:
[0030] (1) Cultivate the seedlings of Shanxiaoju: Select the young fruit of Shanxiaoju with well-developed root system, strong branches and disease resistance, and adopt the tissue culture rapid propagation method to breed robust tissue cultured seedlings with well-developed root system as seedlings; Include the following steps:
[0031] 1) After aseptic disinfection, the ovules were stripped from the young fruit, and the nucellus tissue was cut according to the method of Kachba et al, and inoculated in MT solid medium supplemented with 300 mg / L malt extract to induce callus. The culture conditions were: The temperature is 24°C, the light intensity is 1200lx, and the light time is 17h;
[0032] 2) Transfer the formed callus to the basic medium for subculture to produce embryogenic callus;
[0033] 3) Transfer the embryogenic callus to a medium containing 50g / L sucrose to induce somatic embryos; ...
Embodiment 2
[0048] A kind of artificial planting method of Shanxiaoju comprises the steps:
[0049] (1) Cultivate the seedlings of Shanxiaoju: Select the young fruit of Shanxiaoju with well-developed root system, strong branches and disease resistance, and adopt the tissue culture rapid propagation method to breed robust tissue cultured seedlings with well-developed root system as seedlings; Include the following steps:
[0050] 1) After aseptic disinfection, the ovules were stripped from the young fruit, and the nucellus tissue was cut out according to the method of Kachba et al, and inoculated in MT solid medium supplemented with 300 mg / L malt extract to induce callus tissue, and the culture conditions were temperature 24℃, light intensity 1200lx, light time 18h;
[0051] 2) Transfer the formed callus to the basic medium for subculture to produce embryogenic callus;
[0052] 3) Transfer the embryogenic callus to a medium containing 50g / L sucrose to induce somatic embryos;
[0053] 4)...
Embodiment 3
[0067] A kind of artificial planting method of Shanxiaoju comprises the steps:
[0068] (1) Cultivate the seedlings of Shanxiaoju: Select the young fruit of Shanxiaoju with well-developed root system, strong branches and disease resistance, and adopt the tissue culture rapid propagation method to breed robust tissue cultured seedlings with well-developed root system as seedlings; Include the following steps:
[0069] 1) After aseptic disinfection, the ovules were stripped from the young fruit, and the nucellus tissue was cut out according to the method of Kachba et al, and inoculated in MT solid medium supplemented with 300 mg / L malt extract to induce callus tissue, and the culture conditions were temperature 24℃, light intensity 1200lx, light time 20h;
[0070] 2) Transfer the formed callus to the basic medium for subculture to produce embryogenic callus;
[0071] 3) Transfer the embryogenic callus to a medium containing 50g / L sucrose to induce somatic embryos;
[0072] 4)...
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