Method for quickly evaluating the comprehensive quality of stevia rebaudiana

A stevia, rapid technology, applied in the field of detection, can solve the problems of limited model accuracy and application range, great influence on detection accuracy, poor accuracy of active ingredients, etc., and achieve low cost, fast analysis speed and high accuracy high effect

Active Publication Date: 2019-01-15
CHENGUANG BIOTECH GRP CO LTD
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Problems solved by technology

The quality indicators of stevia include comprehensive indicators such as steviol glycoside content, chlorogenic acid content, flavonoid content, moisture and impurity ratio, among which the chlorogenic acid content and flavonoid content are relatively low, and impurities such as sand and soil have a great impact on its detection accuracy In addition, there are many types of impurities ...

Method used

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  • Method for quickly evaluating the comprehensive quality of stevia rebaudiana
  • Method for quickly evaluating the comprehensive quality of stevia rebaudiana
  • Method for quickly evaluating the comprehensive quality of stevia rebaudiana

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Embodiment 1－20

[0021] Examples 1-20: The method for rapidly evaluating the comprehensive quality of stevia adopts the following specific processes.

[0022] (1) Select a representative stevia sample as a stevia sample, and grind it through a pulverizer of 3000r / min to 4000r / min until all pass through a 40-mesh sieve to obtain a stevia powder sample; the stevia sample comes from Gansu, Ordinary products from different production areas such as Inner Mongolia, Xinjiang, Anhui, Jiangsu, etc. contain different types of impurities, including branches, stones, sand, soil clods, ice slag, etc.

[0023] (2) Turn on the near-infrared spectrometer to preheat for 30 minutes at 25°C, take 20g of stevia powder samples and put them in a rotating sample cup (50mm in diameter, 25mm in depth); use the diffuse reflectance mode of the integrating sphere in continuous wavelength near-infrared scanning to collect spectra , the scanning range is 4300~9000cm -1 , resolution 16cm -1 , to collect the absorption spe...

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Abstract

The invention discloses a method for quickly evaluating the comprehensive quality of stevia rebaudiana, the method steps are as follows: (1) selecting the representative stevia rebaudiana sample as the sample of stevia rebaudiana, and crushing to obtain the stevia rebaudiana powder sample; (2) scanning the stevia rebaudiana powder sample with a near infrared spectrometer to obtain the near infrared spectrum data; (3) the stevioside content, the chlorogenic acid content, the flavone content, the moisture and the impurity ratio of the stevia rebaudiana powder sample are accurately determined after the stevia rebaudiana powder sample is processed; (4) constructing a correction model of the relationship between the stevioside content, the chlorogenic acid content, the flavone content, the moisture, and the impurity ratio and the characteristic near infrared spectrum data of the stevia rebaudiana powder sample; (5) crushing the to-be-measured stevia rebaudiana sample and collecting the nearinfrared spectrum data, obtaining the comprehensive quality of the to-be-measured stevia rebaudiana sample according to the near infrared spectrum correction model constructed in the step (4). The method for quickly evaluating the comprehensive quality of stevia rebaudiana has the characteristics of high analysis speed, high efficiency, low cost and high accuracy.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for rapidly evaluating the comprehensive quality of Stevia rebaudiana. Background technique [0002] Stevia [Stviarebaudiana (Bertoni)] belongs to the Compositae (Composi-tae) Stevia (Stevia) sugar crops, native to the Amambai region in northeastern Paraguay. In the 1970s, it was introduced from abroad to our country for trial planting, and then promoted to the whole country. Stevia leaves mainly contain steviol glycosides, which are about 300 times sweeter than sucrose, and have the characteristics of low calories and no absorption. Secondly, they also contain chlorogenic acid and flavonoids. The traditional method for the determination of steviol glycosides, chlorogenic acid and flavonoids is high performance liquid chromatography (HPLC). Although high-performance liquid chromatography has high precision, it requires complex pretreatment during the detection process, and the ana...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N21/359G01N21/3563
CPCG01N21/3563G01N21/359G01N30/02G01N30/06G01N2021/3572G01N2030/027G01N2030/062
Inventor 刘翠翠石文杰程远欣谷晓红张青考
Owner CHENGUANG BIOTECH GRP CO LTD
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