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75 results about "Stevia connata" patented technology

Stevisalioside A (from the roots of Stevia salicifolia) (Mata et al. 1992), longipinane derivatives in the roots of Stevia connata (Sanchez-Arreola et al. 2000), epoxylabdane diterpenes and a ...

Method for processing stevia rebaudiana dark green tea

The invention relates to a method for producing dark green tea from stevia rebaudiana as a raw material. The method comprises the following steps of: selecting stevia rebaudiana tender shoots or fresh leaves or dried leaves as the raw material; after bacterial inoculation, performing pile fermentation at the temperature ranging from 15 to 35 DEG C for 1-10 d; performing roasting drying after the pile fermentation; and pressing to obtain the stevia rebaudiana dark green tea product. The stevia rebaudiana dark green tea obtained by the method provided by the invention has the effects of reducing blood pressure, resisting diabetes, resisting virus and the like as the stevia rebaudiana leaves contain bioactive substances such as stevioside, caffeoyl quinic acid, and flavone.
Owner:NANJING NORMAL UNIVERSITY

Non-nutritive sweetened beverages with coconut milk

Beverage compositions sweetened with at least one non-nutritive sweetener, such as a compound from the stevia plant, including steviol glycosides, e.g., rebaudiosides such as Rebaudioside A, stevioside, etc. Coconut milk is present in an amount sufficient to enhance the mouthfeel of the beverage, yet not affect the taste. Non-nutritive sweetened beverage compositions, natural beverage compositions, and beverage concentrates incorporating steviol glycosides and coconut milk are also provided. In addition, a method for including coconut milk in a beverage formulation to enhance the mouthfeel of a beverage sweetened with a compound from the stevia plant is provided.
Owner:CONCENTRATE MFG OF IRELAND

Stevia rebaudiana seedling medium and preparation method thereof

The invention relates to a stevia rebaudiana seedling medium for the cultivation of agricultural vegetables and flowers and a preparation method thereof, comprising the following raw materials in parts by weight: 0.5-5 parts of stevia rebaudiana stem, 90-98 parts of turf, and 1-5 parts of leech. The preparation method comprises the following steps: adding the smashed stevia rebaudiana stem, turf powder and leech, mixing uniformly, disinfecting and sterilizing at high temperature so as to prepare the stevia rebaudiana seedling medium. The invention has the following advantages: the seedling medium can provide enough organic nutrient content for the seedling emergence of seeds, thereby promoting the growth of root and restraining root rot and fusarium wilt.
Owner:海城市三星生态农业有限公司

Glucosyl stevia composition

Glucosyl stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The compositions mainly comprise glucosyl derivatives with superior taste characteristics and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.
Owner:PURECIRCLE SDN BHD

Sweet broomwort herbal tea and making method thereof

The invention relates to a sweet broomwort herbal tea and a making method of the sweet broomwort herbal tea. The sweet broomwort herbal tea is prepared from the raw materials such as green tea, tea stems, dianthus, stevia rebaudiana, momordica grosvenori flowers, selfheals and roxburgh roses. The making method comprises the following steps: (1) drying and grinding the green tea and the tea stems; (2) drying and smashing the dianthus and the stevia rebaudiana; (3) selecting the roxburgh rose and drying and grinding the roxburgh rose; (4) drying and grinding the momordica grosvenori flowers and the selfheals; and (5) packaging the product after the raw materials are mixed uniformly. The sweet broomwort herbal tea made by the making method disclosed by the invention has the health-protection effects of relieving pharyngitis, quenching the thirst, promoting digestion, reducing three-highs (high cholesterol, high blood pressure, high blood sugar), improving constipation and the like and in particularly has good health-protection effects on the pharyngitis, the diabetes and the constipation.
Owner:JIANGSU ZHONGHAI HUA HE ENVIRONMENTAL PROTECTION CO LTD

Healthy stevia rebaudiana nutritional powder with effects of lowering lipid and blood pressure and softening blood vessels

The invention discloses healthy stevia rebaudiana nutritional powder with effects of lowering lipid and blood pressure and softening blood vessels. The nutritional powder is prepared from the following raw materials in parts by weight: 70-80 parts of stevia rebaudiana, 30-50 parts of red dates, 400-500 parts of wheat flour, 5-8 parts of radix salviae miltiorrhizae, 5-8 parts of gingko leaves, 15-25 parts of lemon grass, 8-10 parts of licorice roots, 3-5 parts of lucid ganoderma, 3-5 parts of fructus evodiae, 5-8 parts of semen cassiae, 30-50 parts of carrots, 30-50 parts of tomatoes, 10-15 parts of Chinese wolfberry fruit, 10-15 parts of honey and a proper amount of clear water. The healthy stevia rebaudiana nutritional powder has a unique flavor, good taste and comprehensive nutrition, is prepared by decocting multiple carefully chosen medicinal and edible raw materials, is unique in flavor, has the effects of lowering lipid and blood pressure and softening blood vessels, does not contain food additive, and is suitable for people of all ages. The nutritional powder is simple in production process, convenient to manufacture and low in cost, can meet the requirement for industrial production, is convenient to carry and eat, and can be eaten by opening a bag filled with the nutritional powder and mixing the nutritional powder with water.
Owner:黄名玉

Eight-treasure tea for male

The invention discloses an eight-treasure tea for male. The eight-treasure tea for male comprises the following raw materials in percentages by weight: 10-20% of chrysanthemum, 10-15% of grosvener siraitia, 15-20% of barbary wolfberry fruit, 5-10% of Chinese yam, 8-16% of red jujube, 7-15% of raspberry, 3-10% of kudzuvine root and 1-5% of stevia rebaudiana. According to the eight-treasure tea for male provided by the invention, except for the stevia rebaudiana, the others are the plants using as both medicine and food, so the tea has no toxicity and side effects and can be drunk for a long time. The stevia rebaudiana is the edible sweet plant and can be used for replacing the crystal sugar to regulate the sweetness. When the eight-treasure tea for male is mixed with water, the color is orange yellow, the sourness and the sweetness are suitable, and the chrysanthemum fragrance is light. The eight-treasure tea for male has the effects of protecting the liver, nourishing kidney, strengthening spleen, supplementing lung, benefiting stomach, tonifying kidney, reinforcing the kidney, enriching essence, invigorating spleen-stomach, replenishing qi, invigorating splenic yang, relieving diarrhea, clearing away the heart-fire, improving eyesight, clearing away the lung-heat, relieving sore throat and delaying senility; and therefore, the tea is applied to drinking by the male.
Owner:河南菊珍食品饮料有限公司

Poria cocos health drink and preparation method thereof

InactiveCN104770798ASimple methodMaintain beneficial substancesFood preparationAdditive ingredientMedicine
The invention relates to a poria cocos health drink and a preparation method thereof. The poria cocos health drink is prepared from the following raw material constituents in parts by weight: 75-85 parts of poria cocos, 2-7 parts of phyllanthus emblica fruit, 5-15 parts of semen coicis, 4-14 parts of jujube and 1-3 parts of stevia. The preparation method comprises the following steps: grinding the poria cocos, the phyllanthus emblica fruit, the semen coicis, the jujube and the stevia into granular powder, adding the powder into a fermentation tank, stirring evenly, adding 210kg of boiling water of 80 DEG C, stirring evenly, heating up to 30 DEG C, adding bifidobacteria powder in an amount of 0.009 of total weight of raw materials, fermenting for 7 days, preserving heat at 30 DEG C, then, filtering out the fermented material to obtain a filtrate, sterilizing, and bottling. The invention provides a fermented functional drink, the drink contains various nutrition ingredients beneficial for being absorbed by the human body, and has no chemical additives; and the method is simple and practical, and has the advantage of keeping beneficial substances in the raw materials.
Owner:陈裕达

New stevia rebaudiana variety 814011 Puxing No.3 and preparation of high-RM-content stevioside

The invention provides a stevia rebaudiana variant plant which is 814011 Puxing No.3, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9701. The invention also provides a high-RM-content stevioside product, wherein the RM / TSG ratio is greater than 0.08, and the RD / TSG ratio is greater than 0.08.
Owner:PURECIRCLE AMERICAN CORP

Rose lotus leaf tea and preparation method thereof

The invention discloses a rose lotus leaf tea and a preparation method thereof. The rose lotus leaf tea consists of the following raw materials: rose 20-40 parts, semen cassiae 10-24 parts, barley 20-30 parts, lotus leaf 20-40 parts, bitter gourd 3-6 parts, cured Pu'er tea 4-8 parts, stevia rebaudiana 4-10 parts, water chestnut 3-4 parts, corn poppy 3-4 parts, dark plum 15-20 parts, tomato vinegar 10-15 parts, rice bran 20-30 parts, konjaku flour 10-15 parts, and pomegranate seed oil 2-4 parts. In the prepared rose lotus leaf tea, the rice bran is processed and cured, thereby blending the health-care components and improving the taste and flavor of the tea, giving the tea a fragrant rice flavor. The health-care components are easier to be dissolved and absorbed by human body after being organically combined with the tea. Meanwhile, the tea has a golden color and a unique taste; and the tea has effects of eliminating fat, losing weight, beautifying, or the like.
Owner:ANHUI YIBEICHA ELECTRONICS COMMERCE

Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier

ActiveUS20170339994A1Improve flavor profileEnhanced sweetness profileFood sciencePattern perceptionTaste Perceptions
Stevia extracts with selected major steviol glycosides (Reb A, stevioside, Reb D, Reb C) and minor steviol glycosides and glycosylated diterpene derivative plant molecules, derived from Stevia rebaudiana plant are found to improve the perception of flavor and taste perception, which includes the sweet, savory and salty perception in a wide range of food and beverage applications.
Owner:PURECIRCLE USA

Stevia rebaudiana seed seedling cultivation method

The invention relates to the technical field of agricultural planting and discloses a stevia rebaudiana seed seedling cultivation method. The method includes steps: A, seed selection and treatment; B,seedbed preparation; C, seed sowing; D, seedling cultivation period management; E, transplanting. The stevia rebaudiana seed seedling cultivation method has advantages that by adoption of trays for seedling cultivation, the temperature and moisture in a seedling cultivation process are strictly controlled according to characteristics of stevia rebaudiana seeds, nutrient solution is sprayed regularly to guarantee nutrient supply sufficiency, and the seedling survival rate and the germination rate of the stevia rebaudiana seeds are increased.
Owner:明光市大地农业科技有限公司

High-latitude region stevia rebaudiana special-purpose fertilizer

The invention discloses a high-latitude region stevia rebaudiana special-purpose fertilizer. The high-latitude region stevia rebaudiana special-purpose fertilizer comprises the following raw materials in parts by weight: 25-35 parts of ammonium hydrogen phosphate, 5-15 parts of ammonium sulfate, 2-12 parts of rock phosphate, 10-18 parts of a kiln dust potash fertilizer, 0.2-0.8 parts of boron magnesium fertilizer zinc, 0.4-0.9 parts of hexaurea iron(III) trinitrate, 2-6 parts of ammonium magnesium phosphate, 0.5-1.2 parts of ammonium molybdate, 1-2 parts of a boron magnesium fertilizer, 5-10 parts of fermented corn straw, 2-8 parts of earthworm manure, 2-10 parts of Chinese medicine residue, 2-8 parts of bentonite, 20-40 parts of grape seed cake, 15-35 parts of rice bran, 5-15 parts of linen fiber composite clay, 1-4 parts of carbonized corncob powder, 1-2 parts of kaolin, 1-2 parts of fly ash, 2-4 parts of meerschaum, 1-6 parts of carboxymethyl cellulose, and 1-2 parts of brewer's grain. The nutrient element ratio is reasonable, the nutrition requirement problem of stevia rebaudiana can be solved after application, fertilizer utilization rate is increased, and the environment pollution due to fertilizer loss is reduced.
Owner:黄璜

Blending process of stevia rebaudiana bertoni-fragaria ananassa wine

The invention discloses a blending process of stevia rebaudiana bertoni-fragaria ananassa wine. The blending process comprises the following steps: making wine from fresh fragaria ananassa through thefermentation processes of sorting, cleaning, smashing, beating, clarifying, fermenting, clarifying, filtering and the like; steaming fresh sundried stevia rebaudiana bertoni leaves, and extracting stevioside with water; then, blending the fragaria ananassa wine and a stevioside extracting solution according to different proportions to obtain stevia rebaudiana bertoni-fragaria ananassa composite fruit wine. The fruit wine is strong and attractive in fruit fragrance, is fresh, is proper in sour and sweet degrees, is mellow and harmonious, is full in sense of taste, is rich in nutrients, is unique in flavor, is popular, and can relatively well meet the requirements of modern people on the diversity of the fruit wine and health preservation. Meanwhile, the fragaria ananassa fruit wine prepared by fermentation and the stevioside prepared by water extraction are taken as blending raw materials in an experiment, so that the mouthfeel and nutrients of the fragaria ananassa fruit wine can be improved; a composite wine product prepared from the stevia rebaudiana bertoni and the fragaria ananassa is not found yet in the market; a result of the experiment has certain reference value for the development of the novel stevia rebaudiana bertoni-fragaria ananassa composite wine.
Owner:FUYANG NORMAL UNIVERSITY

Composition for promoting growth of stevia rebaudiana, and preparation method for composition

The invention discloses a composition for promoting growth of stevia rebaudiana, and a preparation method for the composition. The composition consists of a fermentation product, monopotassium phosphate, diammonium phosphate, and a trace element complexing agent; and the fermentation product is prepared by the following steps of: uniformly mixing bacillus sp. and lignite, fermenting, and collecting a product to obtain the fermentation product. Through experiment, organism growth regulator special for the stevia rebaudiana is prepared, and the method has the advantages that by applying the growth regulator special for the stevia rebaudiana appropriately, micro-ecological environment of the stevia rebaudiana is effectively improved, micro-ecological balance is adjusted, and nutrient supply conditions are improved.
Owner:CHINA AGRI UNIV

Cuttage seedling substrate of stevia rebaudiana and preparation method thereof

The invention provides a cuttage seedling substrate of stevia rebaudiana and a preparation method thereof. The seedling substrate comprises, by weight, 25-40 parts of fermentation organic fertilizer,15-30 parts of perlite, 5-15 parts of furnace slag, 0.02-0.1 part of fertilizer and 5-10 parts of water-retaining agent. The cuttage seedling substrate of the stevia rebaudiana is short in seedling period and high in survival rate.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Manufacture recipe and manufacture technology of hericium erinaceus smelly taste and bitterness removing products

The present invention discloses a manufacture recipe of hericium erinaceus smelly taste and bitterness removing products. A main point is that plants and edible fungi with fragrant smells are used to remove the smelly taste of the hericium erinaceus. The specific recipe is as follows: 80-100 parts of dried hericium erinaceus products, 15-30 parts of fragrant raw materials, and 0.5-1 part of dried stevia rebaudiana product. The fragrant raw materials are a mixture of one or more of rose flowers, osmanthus fragrans flowers, dried tangerine peels, shii-take or fresh scallion stalk with root hairs, and except that the fresh scallion stalks with the root hairs are fresh products, the other raw materials in the fragrant raw materials are dried raw materials. The products contain the various raw materials with fragrances, all the materials contained in the recipe are medicinally and edibly homogenous, and the products are suitable for populations with various preferences and at different age levels. The natural sweetener of the stevia rebaudiana with low calories and high sweetness is used to adjust the mouthfeel and remove the bitterness, and suitable for the consumption of patients with diabetes. The products are used to boil soup, brew tea, etc. for consumption. A freeze-drying method is used to maximally maintain the nutrients of the hericium erinaceus.
Owner:仙芝科技(福建)股份有限公司 +1

Blueberry health tea and preparation technology thereof

The invention relates to the technical field of blueberry health teas and preparation technologies thereof, and especially relates to a blueberry health tea formula. The formula comprises a blueberry health tea raw material, the blueberry health tea raw material is selected from anyone of or a combination containing two or more of dried blueberry leaves, blueberry stalks, blueberry branches or blueberry fruits, and the particle size of the dried blueberry leaves, blueberry stalks, blueberry branches or blueberry fruits is 50-500 meshes. The blueberry leaves, blueberry stalks, blueberry branches or blueberry fruits can be used as a main raw material to individually prepare the blueberry health tea, and can also be mixed with tea leaves and / or stevia to prepare the blueberry health tea, the blueberry leaves, blueberry stalks, blueberry branches or blueberry fruits are rich in anthocyanidins and have high nutrition values, the tea leaves have various kinds, and the blueberry leaves, blueberry stalks, blueberry branches or blueberry fruits can be matched with different tea leaves to prepare the blueberry health tea with different mouthfeels, so the nutrition values of blueberry are reserved, the delicate fragrance mouthfeel of tea is reserved, and the blueberry health tea is instant. A preparation technology of the blueberry health tea is simple, and is suitable for large-scale industrial application.
Owner:HEYUAN CITY HUARUI BLUEBERRY IND

Heat-preserving fertilizer for stubble of Stevia rebaudiana and preparation method thereof

The invention specifically relates to a heat-preserving fertilizer for the stubble of Stevia rebaudiana and a preparation method thereof, belonging to the technical field of fertilizers for agricultural plantation. The preparation method comprises the following steps: separately weighing plant straw, Stevia rebaudiana residue, Chinese redbud bark, lithospermum and calamus, and carrying out crushing; adding powder obtained after crushing, animal manure, organic household refuse, plant ash and sawdust into a fermentation cylinder, then adding water, Bacillus spp. and aboriginal bacteria and carrying out natural fermentation for 20 to 25 d; and after completion of fermentation, adding bentonite and slag, carrying out uniform mixing under stirring, and then subjecting the obtained mixture to drying, crushing and granulation so as to obtain the heat-preserving fertilizer for the stubble of Stevia rebaudiana. The heat-preserving fertilizer for the stubble of Stevia rebaudiana allows the stubble of Stevia rebaudiana to smoothly pass the winter and provides Stevia rebaudiana with sufficient nutritional components; traditional Chinese medicine components in the fertilizer can effectively kill and eliminate earth-surface insects and prevent Stevia rebaudiana from pest and disease damage; and the preparation method is simple, easy to operate and suitable for promotion and application, and has great application value.
Owner:MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP

Method for making health-care beverage from balsam pear and Stevia rebaudiana

The invention relates to a method for making a health-care beverage from a balsam pear and Stevia rebaudiana. The method is characterized by comprising the following steps: a, cutting the balsam pear into two halves, removing seeds, carrying out drying and pulverization, and then carrying out supercritical extraction of a concentrate for subsequent usage; b, pulverizing Stevia rebaudiana and extracting a concentrate for subsequent usage; and c, weighing 30 to 40% of balsam pear extract obtained in the step a, 13% of Stevia rebaudiana extract, 10% of persimmon vinegar purchased from the market, 7% of citric acid and 30 to 44% of purified water, adding 0.02% of an anti-oxidant VC, and successively carrying out mixing, homogenization, sterilization and filling so as to obtain the health-care beverage.
Owner:ZHENGZHOU GUOSHOU BIOLOGICAL TECH CO LTD

Process method for extracting and purifying stevioside from Stevia rebaudiana (Bertoni) Hemsl.

The invention discloses a process method for extracting and purifying stevioside from Stevia rebaudiana (Bertoni) Hemsl., and particularly relates to the technical field of plant extraction. The method includes the following steps: (1) selecting a beaker of 800 ml, performing washing and sterilizing on the beaker, pouring 200 ml of deionized water into the sterilized beaker, performing heating until boiling, adding 20-30 g of dried leaves of Stevia rebaudiana (Bertoni) Hemsl., and stirring the deionized water in the beaker by using a sterile glass rod. According to the method, ultra-high pressure treatment is performed on a filtrate which is obtained through primary cooking, and leaching is performed, wherein in the process of pressurization, quick entering of a solvent into cells throughmicrochannels of cell walls and other tissue can be promoted by a large osmotic pressure which is generated inside and outside the raw material cells so that the solvent can be in full contact with stevioside, and a certain degree of denatured aggregation of protein and other macromolecular substances is caused through the large pressure, so that the structure of the cell walls and cell membranesbecomes loose; and therefore, the permeability of the cell walls is increased, the mass transfer resistance is reduced greatly, leaching of stevioside is facilitated, and the extraction rate of stevioside is improved.
Owner:郑越

High-purity Rebaudioside D and low-calorie fruit juice containing the same

ActiveUS8574656B2Improved taste property and mouthfeelCosmetic preparationsMilk preparationFruit juiceRebaudioside D
The invention provides methods of purifying Rebaudioside D from the Stevia rebaudiana Bertoni plant extract along with Rebaudioside A. The methods are useful for producing high purity Rebaudioside D and Rebaudioside A. The invention further provides a low-calorie fruit juice containing the purified Rebaudioside D and a process for making the low-calorie fruit juice containing the purified Rebaudioside D.
Owner:PURECIRCLE SDN BHD

Preparation method of litsea-coreana stevia beverage

The invention discloses a preparation method of a litsea-coreana stevia beverage. The preparation method of the litsea-coreana stevia beverage comprises the following steps: S1, selecting high-quality litsea coreana and stevia, carrying out thorough washing by using water, and carrying out thorough air-drying so as to obtain raw materials; S2, weighing the following raw materials in percentage by mass: 0.6-0.8% litsea coreana, 15-20% of stevia, and the balance water; S3, crushing the litsea coreana and stevia in a crushing machine so as to obtain powdered litsea coreana and stevia; S4, sieving the litsea coreana powder and the stevia powder by using a 16-mesh sieve so as to obtain tea powder; S5, adding an appropriate amount of water into an extraction tank, carrying out heating until the water is 75-90 DEG C, putting the collected litsea coreana powder and stevia powder into the extraction tank according to percentage by mass, and carrying out uniform stirring and full dissolving so as to obtain a solution; S6, continuing heating the solution for 12-20 minutes, and sieving the heated solution by using a 200-mesh sieve so as to obtain a final filtrate; S7, adding the final filtrate into the extraction tank, carrying out heating until the temperature reaches 60 DEG C, and carrying out vacuum concentration so as to obtain a condensate; and S8, filling the condensate, and carrying out high-pressure sterilization so as to obtain a final product of the beverage.
Owner:四川蜀妹子茶叶有限公司

Partridge flower plant herbal tea beverage and preparation method thereof

The invention discloses a preparation method of a partridge flower plant herbal tea beverage. The method comprises the following steps: taking and placing 9-11 parts of partridge tea leaves and 0.8-1.2 parts of green tea leaves in a filter bag, and conducting extraction in 30-35 parts of water for 10-15 minutes to obtain partridge tea juice; taking, heating and boiling 7-10 parts of wax gourd water, cooling the water to 40-45 DEG C, putting 1-3 parts of oregano leaves, 3-5 parts of chrysanthemums and 1-3 parts of mint, and conducting extraction with covering for 1-1.5h to obtain a flower plantfragrant liquid; adding 4-8 parts of lalang grass rhizomes into 7-10 parts of wax gourd water, conducting decocting for 0.5-1h at a high temperature, and when the temperature drops to 90-100 DEG C, conducting extraction with 0.3-0.7 part of stevia and 0.3-0.7 part of green grosvenor momordica fruits to obtain a flower plant sweet liquid; and mixing the partridge tea juice, the flower plant fragrant liquid and the flower plant sweet liquid, conducting stirring at high speed, and performing filtration, homogenization, enzyme inactivation, vacuum degassing and pasteurization to obtain the herbaltea. The herbal tea is obtained by subjecting the partridge tea juice, the flower plant fragrant liquid and the flower plant sweet liquid which are obtained by raw material sorting treatment to mixing, filtering and homogenization. The method is simple in process and convenient for batch production, and uses wax gourd as a carrier which adsorbs the aroma peculiar to Chinese herbal plants. The beverage has a unique taste and flavor, and has cool, refreshing and health-care efficacy.
Owner:海南香世界科技开发有限公司

Method for obtaining callus tissue of stevia rebaudiana bertoni

The invention belongs to the field of plant cell culture, and particularly relates to a method for obtaining callus tissue of stevia rebaudiana bertoni. The method for obtaining the callus tissue of the stevia rebaudiana bertoni comprises steps as follows: induced culture of stomatal guard cells of the stevia rebaudiana bertoni, suspension culture and induced culture of the callus tissue. With theadoption of the method for obtaining the callus tissue of the stevia rebaudiana bertoni, a large quantity of epithelial fragments containing the stomatal guard cells can be quickly obtained, a high-purity suspension cell culture system is rapidly established, cell screening and culture time is shortened, the obtained callus tissue is high in quality, the percentage of embryonic callus is high, and weight increment is fast.
Owner:BEIJING AGRO BIOTECH RES CENT

Method for quickly evaluating the comprehensive quality of stevia rebaudiana

The invention discloses a method for quickly evaluating the comprehensive quality of stevia rebaudiana, the method steps are as follows: (1) selecting the representative stevia rebaudiana sample as the sample of stevia rebaudiana, and crushing to obtain the stevia rebaudiana powder sample; (2) scanning the stevia rebaudiana powder sample with a near infrared spectrometer to obtain the near infrared spectrum data; (3) the stevioside content, the chlorogenic acid content, the flavone content, the moisture and the impurity ratio of the stevia rebaudiana powder sample are accurately determined after the stevia rebaudiana powder sample is processed; (4) constructing a correction model of the relationship between the stevioside content, the chlorogenic acid content, the flavone content, the moisture, and the impurity ratio and the characteristic near infrared spectrum data of the stevia rebaudiana powder sample; (5) crushing the to-be-measured stevia rebaudiana sample and collecting the nearinfrared spectrum data, obtaining the comprehensive quality of the to-be-measured stevia rebaudiana sample according to the near infrared spectrum correction model constructed in the step (4). The method for quickly evaluating the comprehensive quality of stevia rebaudiana has the characteristics of high analysis speed, high efficiency, low cost and high accuracy.
Owner:CHENGUANG BIOTECH GRP CO LTD

Enzyme-assisted extraction of steviol glycosides from the leaves of Stevia rebaudiana Bertoni

The invention relates to the development of a simple and eco-friendly process of extraction of sweet glycosides: steviosides from the leaves of Stevia rebaudiana. In particular, certain embodiments provide enhanced isolation of steviosides by enzyme assisted extraction, in combination with metal salts, followed by pressurized aqueous extraction. Thus obtained stevia extract was further processed for clarification and purification using multi-stage membrane filters (micro-filtration, ultra-filtration and nano membrane filtration) to obtain high purity steviosides from the Stevia leaves. This improved process confirms the isolation of high purity steviol glycosides i.e., Stevioside and Rebaudioside-A as the final products, with improved organoleptic properties. Thus, this superior process helps in obtaining pure steviosides from the Stevia leaf extract, without impurities and obnoxious residues, using easy operational technology without any damage to the environment.
Owner:COUNCIL OF SCI & IND RES

Extraction method for providing an organic certifiable Stevia rebaudiana extract

A simple extraction method for preparing an organic certifiable stevia extract with high sweetness and optionally with antioxidant properties is provided. The extraction method involves extraction of stevia leaves that have been dried and grinded with a hot solvent generally regarded as safe (GRAS), allowing the dissolution of the sweetening compounds naturally present therein. These sweetening compounds include steviosides and rebaudioside A, which are purified from the extracts by two or more successive chromatographic purifications steps. The sweetening compounds are then eluted with an appropriate elution solvent. The simplicity and organic certification of the method provide advantages over the methods currently employed in the stevia industry.
Owner:JUSTBIO

Method for preparing stevia whole stevioside and stevia whole flavone at the same time

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing oneor several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].
Owner:石任兵 +1
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