Process method for extracting and purifying stevioside from Stevia rebaudiana (Bertoni) Hemsl.

A technology of stevioside and process method, which is applied in the field of plant extraction, can solve the problems of long extraction time, low product yield, large water consumption and large amount of organic wastewater, and achieve the reduction of organic wastewater generation, high product purity and yield , The effect of low equipment requirements

Inactive Publication Date: 2019-12-20
郑越
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing extraction process of steviol glycosides takes a long time, consumes a lot of water, produces a large amount of organic wastewater, and has low product yield, which has become a pain point in the industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A process method for extracting and purifying stevioside from Stevia, comprising the following steps:

[0024] Step 1, select an 800ml beaker to wash and sterilize it, pour 200ml of deionized water into the sterilized beaker, heat and boil, add 20g of dried Stevia leaves, and use a sterile glass rod to stir the deionized water in the beaker. water, so that deionized water completely submerged the dried stevia leaves, and after cooking for 30 minutes, it was cooled to room temperature, and the scum and impurities in the solution were filtered to obtain a filtrate;

[0025] Step 2, pour the filtrate into the crucible, place it in an ultra-high pressure environment of 100Mpa, maintain the pressure for 3 minutes, and then extract the filtrate to obtain the extract;

[0026] Step 3, pour the obtained extract into a beaker, add deionized water with a mass ratio of 3:1 to the extract, heat to 80 degrees Celsius under a low pressure environment of 4900Pa and keep the temperatur...

Embodiment 2

[0031] A process method for extracting and purifying stevioside from Stevia, comprising the following steps:

[0032] Step 1, select an 800ml beaker to wash and sterilize it, pour 200ml of deionized water into the sterilized beaker, heat and boil, add 25g of dried Stevia leaves, and use a sterile glass rod to stir the deionized water in the beaker. water, so that deionized water completely submerged the dried stevia leaves, after cooking for 40 minutes, cooled to room temperature, and filtered to remove scum and impurities in the solution to obtain a filtrate;

[0033] Step 2, pour the filtrate into the crucible, place it in an ultra-high pressure environment of 110Mpa, maintain the pressure for 4 minutes, and then extract the filtrate to obtain the extract;

[0034] Step 3, pour the obtained extract into a beaker, add deionized water with a mass ratio of 3:1 to the extract, heat to 80 degrees Celsius under a low pressure environment of 4900Pa and keep the temperature for 50 m...

Embodiment 3

[0039] A process for extracting and purifying steviol glycosides from Stevia rebaudiana, comprising the following steps:

[0040] Step 1: Select an 800ml beaker to clean and sterilize it, pour 200ml of deionized water into the sterilized beaker, heat to boil, add 30g of stevia dry leaves, use a sterile glass rod to stir the deionized water in the beaker Water, so that the deionized water is completely submerged in the dry stevia leaves, after cooking for 50 minutes, cool to room temperature, and filter out the scum and impurities in the solution to obtain the filtrate;

[0041] Step 2, pouring the filtrate into the crucible, placing it in an ultra-high pressure environment of 120Mpa, keeping the pressure for 5 minutes, and then extracting the filtrate to obtain the extract;

[0042] Step 3: Pour the obtained extract into a beaker, add deionized water with a mass ratio of 3:1 to the extract, heat to 80 degrees Celsius under a low pressure environment of 4900Pa and keep it warm ...

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PUM

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Abstract

The invention discloses a process method for extracting and purifying stevioside from Stevia rebaudiana (Bertoni) Hemsl., and particularly relates to the technical field of plant extraction. The method includes the following steps: (1) selecting a beaker of 800 ml, performing washing and sterilizing on the beaker, pouring 200 ml of deionized water into the sterilized beaker, performing heating until boiling, adding 20-30 g of dried leaves of Stevia rebaudiana (Bertoni) Hemsl., and stirring the deionized water in the beaker by using a sterile glass rod. According to the method, ultra-high pressure treatment is performed on a filtrate which is obtained through primary cooking, and leaching is performed, wherein in the process of pressurization, quick entering of a solvent into cells throughmicrochannels of cell walls and other tissue can be promoted by a large osmotic pressure which is generated inside and outside the raw material cells so that the solvent can be in full contact with stevioside, and a certain degree of denatured aggregation of protein and other macromolecular substances is caused through the large pressure, so that the structure of the cell walls and cell membranesbecomes loose; and therefore, the permeability of the cell walls is increased, the mass transfer resistance is reduced greatly, leaching of stevioside is facilitated, and the extraction rate of stevioside is improved.

Description

technical field [0001] The present invention relates to the technical field of plant extraction, and more particularly, to a process method for extracting and purifying stevioside from Stevia rebaudiana. Background technique [0002] Stevia (scientific name: Stevia rebaudiana (Bertoni) Hemsl.) is a perennial herb of the Asteraceae, Stevia genus. Plant height 1-1.3 meters. Root shoots are hypertrophic, 50-60, up to 25 cm long. Stem erect, base tip lignified, upper part tender, densely short hairs, corolla base light purple or white, upper part white. Achenes linear, slightly flat, brown, with pappus. The flowering period is July-September, and the fruiting period is September-November. It is native to the alpine grasslands of the border between Paraguay and Brazil in South America. This species likes to grow in warm and humid environments and is sensitive to light. Since 1977, it has been introduced and cultivated in Beijing, Hebei, Shaanxi, Jiangsu, Anhui, Fujian, Huna...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/256
CPCC07H1/08C07H15/256
Inventor 郑越
Owner 郑越
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