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New stevia rebaudiana variety 814011 Puxing No.3 and preparation of high-RM-content stevioside

A technology of steviol glycosides and stevia, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of inability to meet customer needs, low product yield, and unfavorable cost control.

Active Publication Date: 2016-08-17
PURECIRCLE AMERICAN CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the low content of RM in Stevia rebaudiana, in the past, the only way to obtain high-purity RM was repeated recrystallization. The disadvantage of repeated recrystallization is that the product yield is low, which is not conducive to cost control; Large-scale production, unable to meet customer needs

Method used

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  • New stevia rebaudiana variety 814011 Puxing No.3 and preparation of high-RM-content stevioside
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  • New stevia rebaudiana variety 814011 Puxing No.3 and preparation of high-RM-content stevioside

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Breeding of New Line 814011 Puxing 3 (814011PC Star 3)

[0037] Parental Traits for Cross Breeding :

[0038] Female parent 1: Eirete: a new stevia variety introduced from Paraguay in 2010, with high total steviol glycoside (TSG) content (greater than 20%). The height of the plant is 80cm, the leaves are lanceolate, the edges of the leaves have obvious serrations, the color is dark green, the content of Reb A in the dry leaves is 11.30%, the content of St is 10.4%, and each plant has 6-10 branches.

[0039] Male parent AKH L1: a new stevia variety introduced from Paraguay in 2010, with high Reb A content. This variety is a late-maturing variety with a plant height of 51-70 cm. It grows slowly in the early stage of field planting and vigorous in the middle and late stages. The growth period of a field crop is 135-150 days. The leaf color is light green / yellow green. More than 200 kilograms of dried leaves per mu. The content of Reb A in dry leaves is 11.5%, and tha...

Embodiment 2

[0071] Preparation of steviol glycoside products with high RM content

[0072] (a) Pretreatment: Stevia leaves are mixed with 50°C soft water at a weight ratio of 1:15 to make a steviol glycoside extract. After two processes of flocculation and sedimentation and plate and frame filter press, the filtrate after plate and frame filtration enters the next step process;

[0073] (b) Primary adsorption and desorption: Adsorb the active ingredients of steviol glycosides in the above-mentioned extract with a primary adsorption resin, treat the resin adsorbed with the active ingredients of steviol glycosides with sodium hydroxide solution and hydrochloric acid solution, rinse the resin with pure water until the effluent pH When the value reaches 7 o'clock, then carry out desorption with ethanol solution to obtain a desorption solution with a glycoside content of 84.5%, and the desorption solution is de-alcoholized and enters the next process;

[0074] (c) Primary ion exchange: the de...

Embodiment 3

[0080] Preparation of steviol glycoside products with high RM content

[0081] (a) Pretreatment: Stevia leaves are mixed with 70°C soft water at a weight ratio of 1:20 to make a steviol glycoside extract. After two processes of flocculation and sedimentation and plate and frame filter press, the filtrate after plate and frame filtration enters the next step process;

[0082](b) Primary adsorption and desorption: use a primary adsorption resin to adsorb the active ingredients of steviol glycosides in the above-mentioned desalination filtrate, treat the resin adsorbed with the active ingredients of steviol glycosides with sodium hydroxide solution and hydrochloric acid solution, and then desorb with ethanol solution to obtain glycosides Measure 82.6% of the desorption solution, and the desorption solution will go to the next process after alcohol removal;

[0083] (c) Primary ion exchange: the de-alcoholic desorption solution is sequentially removed from impurities by primary i...

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Abstract

The invention provides a stevia rebaudiana variant plant which is 814011 Puxing No.3, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9701. The invention also provides a high-RM-content stevioside product, wherein the RM / TSG ratio is greater than 0.08, and the RD / TSG ratio is greater than 0.08.

Description

technical field [0001] The invention relates to a new stevia variety, which contains high RM content and can be produced by continuous cultivation of preserved callus; it relates to a method for preparing steviol glycosides with high RM content from the stevia dry leaves of the variety. Background technique [0002] Stevia (Stevia), scientifically named Stevia Rebaudiana Bertoni, is a perennial herb of Compositae, native to Paraguay, South America. Its leaves contain steviol glycosides, which are 150-300 times sweeter than sucrose. It is a new type of natural sweetener with sweetness, low calorie, good taste, safety and non-toxicity, which can be widely used in food, beverage, medicine, daily chemical industry and other industries. [0003] Steviol glycoside is a multi-component glycoside mixture, and the glycoside component and content determine the sweetness and taste of the mixture. Stevioside in stevia contains 13 components, namely stevioside A (Rebaudioside A, referre...

Claims

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Application Information

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IPC IPC(8): A01H5/00A01H1/02A01H4/00C07H15/256C07H1/08A01H5/12
CPCC12Q1/6895C12Q2600/13C12Q2600/156A01H5/12A01H6/1488
Inventor 奥维迪·玛珂善李善汪卜宇成
Owner PURECIRCLE AMERICAN CORP
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