A kind of Burkholderia and its application
A technology of Burkholderia and pathogenic bacteria, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of pesticide residues, poor control effect, pathogenic bacteria resistance, etc., and achieve good antibacterial effect
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[0037] 1. Isolation and purification of strains
[0038] Randomly select 5 points in the rice planting area of the Fuyang base of the China Rice Research Institute to dig rice roots, gently wash the floating soil on the root system with running water, and wash the soil closely attached to the root system by ultrasonic vibration. After gradient dilution of the above soil suspension, spread it on the LB culture dish, place the culture dish in a constant temperature incubator at 28°C, and pick colonies of different forms to streak and purify on fresh LB medium plates whenever the colonies grow. , and then pick a single colony and store it on a LB solid slope at 4°C for short-term activity determination. At the same time, a single colony was inoculated with LB liquid, and after overnight shaking culture at 28°C, the bacterial liquid was mixed with 40% sterilized glycerol in equal volumes and stored at -80°C for a long time.
[0039] Among the many isolated strains, a strain wit...
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