Method for in vitro immature embryo induction and plant regeneration of Zilian

Abstract

The invention discloses an in-vitro immature embryo induction and plant regeneration method of seed lotus. The method comprises the following steps: firstly inoculating an immature embryo onto a lotusembryo culture medium to be cultured for 3 to 4 weeks in a light-proof manner, then transferring the induced emerging lotus embryo tissue onto a lotus embryo culture medium to perform subculture, performing dark culture at the temperature of 24 to 26 DEG C, transferring the subcultured good lotus embryo tissue onto a lotus embryo culture medium to perform light culture, finally transferring the lotus embryo tissue with good growth state and becoming green onto a novel seedling raising culture medium to be cultured, so as to form a lotus test-tube seedling. By adopting the in-vitro immature embryo induction and plant regeneration method of the seed lotus, the problem that the lotus distant hybridization offspring fruiting rate is low can be overcome, the survival rate of the lotus distanthybridization offspring can be increased, and the method has certain application value on improving the lotus inheritance and breeding the varieties.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for in vitro immature embryo induction and plant regeneration of seed lotus. Background technique [0002] Lotus (Nelumbo nucifera Gaertn.) belongs to the Nymphaeaceae (Nymphaeaceae) Nelumbo Adans plant, and is a perennial aquatic vegetable crop. Lotus plants include two species: Chinese lotus (N.nucifera Gaertn.) and American yellow lotus (N.lutea Pers.). China is one of the centers of origin of lotus. Lotus has a long history of cultivation and domestication in my country. In the ancient Chinese book "The Book of Songs", there are "Fusu in the mountains, and lotus in Xi'an", "There are cattails and lotus in the lake of Bize". "The verse. The cultivation area of ​​lotus is extensive, and resource type is abundant, and places such as the south of the Yellow River and the Yangtze River Basin of my country are most suitable for the growth of lotus (Kong Qingdong, Chine...

AI Technical Summary

Problems solved by technology

[0005] (2) Excavating lotus rhizomes is time-consuming and laborious, and the number of shoot tips on a lotus rhizome is limited, which leads to low starting efficiency of lotus shoot tip tissue culture and rapid propagation, and the rapid propagation coefficient of lotus shoot tips is very low. Low;

[0006] (3) Use young lotus embryos as explants for tissue culture and rapid propagation. At this stage, it only stops at inducing cluster buds and rooting. Few of them can become seedlings, and their popularization and application are limited.

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