31results about How to "The experimental steps are simple" patented technology

The invention provides a method for in-situ growth of CoP nanoparticles on a g-C3N4 surface and application. The method is mainly characterized in that in-situ growth of Co(OH)2 on carbon nitride is performed according to an in-situ loading method, and then sodium hypophosphite is decomposed under the protection of an inert atmosphere to release PH3 gas to generate CoP. By adoption of the method, the CoP nanoparticles can be uniformly loaded on carbon nitride, and a composite CoP / g-C3N4 photocatalyst is obtained finally. Easiness in raw material acquisition and simplicity in operation are realized, and the composite photocatalyst is innovatively applied to photocatalysis production of hydrogen peroxide; as an auxiliary catalyst, CoP is capable of enhancing visible light absorbability, and owing to the metalloid property of CoP, quick separation of electrons and holes can be realized, and absorbed light can be used more efficiently, and finally catalysis activity of carbon nitride being a main catalyst in photocatalysis production of hydrogen peroxide is effectively improved.

The invention provides a preparation method of a carbon nitride quantum dots-modified inverted opal g-C3N4 catalyst. The carbon nitride quantum dots-modified inverted opal g-C3N4 catalyst can be wellused to degrade organic wastewaters, such as phenols, under the action of visible light. Three-dimensionally and orderly arranged silica (SiO2) is used as a hard template and dihydrogen diamine (DCDA)as a precursor to synthesize inverted opal g-C3N4 (CN IO) photonic crystal structure. The CN IO is subjected to loading modification of carbon nitride quantum dots (CNQDs), and optimal loading capacity is investigated. The preparation method enables the large pore diameter of inverted opal structure to be controlled by changing the particle size of the hard template; loading capacity of CNQDs iscontrolled by changing the addition of the CNQDs solution. By applying the catalyst herein to degrade phenolic wastewaters, it is discovered that the catalyst can quickly degrade organic pollutants under the action of visible light; the catalyst herein has better catalytic activity than common carbon nitride (bulk-CN) and CN IO materials.

The invention provides a preparation method and application of a lignin-doped metal organic framework (MOF)-derived carbon-iron composite material. The material can be used as an adsorbent for various antibiotics and heavy metal hexavalent chromium ions under wide pH conditions. The material is synthesized by taking a mixture of MOF and lignin as a precursor and adopting a one-step pyrolysis method, and iron nanoparticles in the obtained material are uniformly loaded on the surface of an ultrathin carbon nanosheet with a porous structure. The doping of lignin improves the specific surface area and graphitization degree of the carbon material, can effectively enhance the transmission and capture capabilities of substances, is beneficial to dispersion of iron nanoparticles, minimizes particle aggregation, provides additional stability for the iron nanoparticles, and is used as a secondary adsorption site of antibiotics and heavy metal hexavalent chromium ions. Meanwhile, the material has magnetism and can be quickly and conveniently recycled from a water phase. When being applied to adsorption of antibiotics and heavy metal hexavalent chromium ions, the composite material shows excellent adsorption performance.

The invention discloses a method for synthesizing a Schiff base compound containing camphenyl. The method includes the following specific synthetic steps that camphorenal is used as a raw material, an aromatic amine compound is added in a reaction device containing organic solvent, the camphorenal and aromatic amine are fed according to the molar ratio of 1:1-1.1, stirring reflux is conducted for 2-3 hours, a reacted product is purified through solvent, and then drying is carried out, so that the Schiff base compound containing the camphenyl is obtained. According to the preparation method, the camphorenal is used as the raw material to be reacted with the aromatic amine compound so that the Schiff base compound can be obtained, camphene radicals are introduced into Schiff base, and finally the aromatic amine Schiff base containing the camphenyl is obtained. The experimental steps are simple and convenient, the reaction condition is moderate, time is short, the yield is high, safety and environmental protection are achieved, aftertreatment is convenient, and secondary pollution is small.

The invention provides a flammable gas explosion system and method in a small-sized pipeline. The system comprises a flammable gas explosion experiment section in the small-sized pipeline; an acetylene driving section used for detonating a non-sensitive mixed gas; a hydrogen bottle, a nitrogen bottle, a carbon monoxide bottle, an oxygen bottle and an acetylene bottle which are used for providing acombustible gas and air components; a vacuum system used for pumping the experiment section and related pipelines to a vacuum state; a gas mixing system for stirring a flammable mixed gas in the experiment section to be uniformly mixed; and supporting power distribution equipment and datum measurement and acquisition equipment. The experimental procedure is as follows: after an experiment systemis pumped to the vacuum state, according to requirements of working conditions, a certain ratio of the flammable mixed gas is poured into the experiment section, ignition is started and data begin tobe recorded, after explosion is completed, a gas is discharged, and the experiment is finished. The explosion system provided by the invention adopts acetylene and oxygen as detonating gases, and hashigh safety; and simulated gas components are more, and the flammable gas components in a containment after a serious accident of a nuclear reactor happens are more realistically simulated.

The invention discloses a kit for rapidly detecting the SNP site of a Y chromosome and a method, and the method is applied to the rapid identification of Y chromosome haplotype pedigrees of male individuals in forensic science. The rapid detection kit for the identification of the characteristic sites of the Y chromosome haplotype pedigrees of East Asian males includes primers corresponding to theSNP characteristic sites used for amplification detection of the Y chromosomes and probes of two mutation type sequence characteristics representing the SNP sites. The using method includes adding asample into plates having 96 or 384 pores, and performing an amplification reaction in a PCR instrument after a PCR reaction system is well mixed; putting the plates into the quantitative PCR instrument having an SDS scanning function to read a fluorescence signal after the amplification is finished; and automatically analyzing the Y-SNP haplotype of the sample by the instrument according to the fluorescence signal, and outputting a result in a spreadsheet. Through the kit, the detection can be completed by one step amplification, purification and template preparation are not needed, and the detection process of the Y-SNP can be simplified.

The invention belongs to the technical field of gene engineering, and particularly relates to a method for acquiring single cell of B cell surface receptor (BCR) or an antibody gene of an anti-N-methyl-D-aspartic acid receptor encephalitis patient, and further discloses immune repertoire research of B cells of the anti-N-methyl-D-aspartic acid receptor encephalitis patient. According to a method for amplifying a variable region and a constant region of the single B cell humanized antibody gene and a method based on a single B cell reverse transcription and BCR amplification, the characteristicof antigen-antibody specific binding is used for the first time, a positive B cell combined with a pathogenic antigen NR1 protein subunit for resisting N-methyl-D-aspartic acid receptor encephalitisis screened out in advance by using a flow cytometry, the interference of other non-positive BCR / antibody information is reduced, the unnecessary workload is reduced, the correlation between the finally obtained antibody information and the disease is stronger, the efficiency of capturing the single-cell B cell antibody sequence is higher, the experiment steps are simpler and more convenient, andthe operability is stronger.

The invention discloses an N-substituted gardenamide A derivative and a synthesis method and application of the N-substituted gardenamide A derivative. The N-substituted gardenamide A derivative has a structure as the general formula (I). The starting material of the N-substituted gardenamide A derivative is genipin, first, hydroxide radicals are selectively protected and converted into lactone under the action of oxidant, and then the lactone directly reacts with an amine compound to obtain an adduct after acid treatment. The N-substituted gardenamide A derivative and the synthesis method of the N-substituted gardenamide A derivative are not reported in literature. Due to the synthesis method of the N-substituted gardenamide A derivative, the application of the N-substituted gardenamide A derivative to preparation of drugs for treating neurodegenerative diseases, in particular to drugs for treating senile dementia is reported for the first time. The synthesis method has the advantages of being mild in reaction conditions, simple and convenient in experimental procedure, high in yield, high in product purity, economical, practical and the like. The general formula (I) is as follows.

The invention discloses a high-sensitivity nucleic acid sequence detection composition and a detection method. The composition comprises a first upstream primer, a second upstream primer, a probe anda downstream primer, wherein the first upstream primer is sequentially composed of the following two parts from a 5' end to a 3' end of (1) a non-matching area, wherein complementary pairing does notoccur with a target nucleic acid sequence; the sequence of the non-matching area from the 5' end to the 3' end comprises the same sequence as the second upstream primer, and the same sequence as the probe; and (2) a matching area, wherein complementary pairing occurs with the target nucleic acid sequence. Compared with the prior art, the primer probe composition provided by the invention has the remarkable advantages of high sensitivity, high specificity, low cost and the like, is suitable for nucleic acid detection of various sample types, and has extremely high application value.

The invention relates to a preparation and using method of a cyclophosphazene flame retardant. A cyclophosphazene flame retardant is modified by ester groups, and the cyclophosphazene flame retardanthas good compatibility with polythylene terephthalate (PET) and polybutylene terephthalate (PBT) materials according to the theory of like dissolves like. The technical scheme adopted by the inventionis as follows: side group functionalization is carried out on hexachlorocyclotriphosphazene through a one-step method for synthesizing hexakis(p-ethyl acetate phenoxyl)cyclophosphazene. According tothe invention, flame retardant modification is carried out on epoxy resin, PET and PBT through the cyclophosphazene flame retardant. The limit oxygen index of the modified epoxy resin is in a range of27.3%-29.5%, the flame retardant rating of modified PET is UL94V-0, the limit oxygen index of modified PET is in a range of 22.5%-25.8%, and the flame-retardant rating of modified PET is UL94V-1.

The invention relates to a method for preparing and modifying an HEK293T cell, a modified HEK293T cell, a method for preparing drug-loaded exosome and the drug-loaded exosome. The method for preparingand modifying the HEK293T cell comprises the following steps: S1, constructing plasmids: separately constructing three plasmids, namely pcDNA3.1-STEAP3, pcDNA3.1-SDC4 and pcDNA3.1-NadB fragments by using a pcDNA3.1 expression vector as a plasmid skeleton; and S2, transfecting the three plasmids in S1 into HEK293T cells according to ratios of (1-3):(1-3):(1-3), and screening a stable cell strain to obtain a modified HEK293T cell strain. By adopting the technical scheme, due to the synergistic effect of the three plasmids, the HEK293T cells with larger exocrine secretion can be reformed, so that the exocrine secretion is further improved, and more choices are provided for clinical tests.

The invention discloses a high-effective construction method for a T carrier based on a PCR method. The invention is that a pair of oligodeoxynucleotide sections which contain specific restriction enzyme sites are introduced into an initiation carrier (such as pUC18); the restriction enzyme site is characterized in that the corresponding enzyme (such as EcoRV) can generate blunt end, and the baseof fro and post tangency point are respectively T and A; the initiation carrier is linearized under the function of the enzyme which is then used as a PCR template; designed primers produce two single chain through single-primed PCR or asymmetric PCR amplification, the two single-chains are annealed so as to form the T carrier. The method for preparing the T carrier is simple and fast; the produced T carrier can effectively clone PCR products containing tail A, and the non-reconstitution background is nearly zero.

The invention provides a preparation method and application of a CoS modified Ti3C2MXene material. The CoS modified Ti3C2MXene material can serve as a peroxymonosulfate (PMS) activating agent to degrade organic pollutants represented by rhodamine B (RhB) under the wide pH condition. According to the material, CoS nanoparticles are fixed on a Ti3C2MXene film through a solvothermal method to form the composite material. In the obtained material, CoS nanoparticles are uniformly loaded on the surface of the Ti3C2MXene nanosheet. Wherein MXene is used as a substrate, so that the transmission and capture capabilities of substances can be effectively enhanced, dispersion of cobalt nanoparticles is facilitated, particle aggregation is minimized, and additional stability is provided for the cobalt nanoparticles. Compared with CoS and Ti < 3 > C < 2 > MXene monomer materials, the performance of the composite material loaded with the CoS nano particles is greatly improved. By applying the material to a photo-Fenton-like system, it is found that the material can efficiently and rapidly degrade a high-chroma pollutant rhodamine B (RhB), and the material has a good application prospect in the field of environmental governance.

The invention discloses a quantum dot (semiconductor nanocrystal) with near-infrared luminescence and magnetism as well as a preparation method and application of the quantum dot. The quantum dot has a core-shell crystal structure, the core is a semiconductor crystal material which is near-infrared luminous and does not contain heavy metals, the shell is a magnetic broad-band gap semiconductor crystal material which does not contain heavy metals, the conditions are mild, the process is simple, the operation is simple and convenient, the cost is low, and the method is flexible. The size of the nanocrystal is 2-15nm, the near-infrared emission peak position is within the range of 650-1500nm and can be randomly modulated according to the size and composition, and the highest near-infrared luminous efficiency reaches 80%. Wherein the magnetic ions in the crystal lattice of the shell layer are stably marked, the content of the magnetic ions can be randomly modulated, and the composite material has high longitudinal magnetic resonance relaxation rate and excellent magnetic resonance imaging performance, and can be used for constructing a near-infrared optical / magnetic resonance imaging multifunctional probe for near-infrared luminescence / magnetic resonance multi-mode biomedical imaging.

The invention discloses a high-sensitivity and high-specificity composition for nucleic acid detection and application of the composition in a detection kit. The composition comprises a first upstream primer F1, a second upstream primer F2, a probe P, a downstream primer R and oligonucleotide F1C, the first upstream primer consists of the following two parts in sequence from the 5'end to the 3 'end: (1) a mismatching region which is not complementarily paired with a target nucleic acid sequence; the sequence of the mismatching region comprises a sequence which is the same as the sequence of the second upstream primer and a sequence which is the same as the sequence of the probe from the 5'end to the 3 'end; and (2) a matching region: performing complementary pairing with a target nucleic acid sequence. Compared with the prior art, the primer probe composition provided by the invention has the remarkable advantages of high sensitivity, good specificity, low cost and the like, is suitable for nucleic acid detection of various sample types, and has extremely high application value.

The invention provides a high-efficiency recombined adenovirus containing an ndrg2 gene and the pharmic purpose thereof. The recombined adenovirus contains the ndrg2 gene, a Gateway system and a Virapower TM Adenoviral Expression system are utilized in the construction process, and the adenovirus is packed by a recombination method in vitro. After being cloned to an introduction carrier, a target gene can be efficiently and quickly cloned to other carriers of a receptor by specific recombination sites on the carriers and recombinase without depending on a restriction enzyme. The package of the adenovirus in vitro simplifies the experiment steps, shortens the operation time, and has high multiplication efficiency, high transgene efficiency and high virus yield efficiency as well as the transduction efficiency as high as 100 percent. The invention solves the technical problem of recombination and package of the ndrg2 gene in vitro as well as probes and determines the lethal effect of the high-efficiency recombined adenovirus to brain glioma cells and cells of lung cancer, liver cancer, gastric cancer and colon cancer. The high-efficiency recombined adenovirus can be used as a medicament for treating brain glioma, long cancer, liver cancer, gastric cancer and colon cancer.

The invention discloses a basalt fiber surface treatment device and method.The treatment device comprises a drying oven, a treatment liquid atomization mechanism, a control signal generator and a basalt fiber fixing assembly, the treatment liquid atomization mechanism is installed at the top of the drying oven, and the basalt fiber fixing assembly is installed on one side plate of the drying oven; the control signal generator is installed on the edge of the other side plate of the drying oven, the working end of the basalt fiber fixing assembly corresponds to the atomization spraying end of the treating fluid atomization mechanism, and the treating fluid atomization mechanism and the basalt fiber fixing assembly are electrically connected with the control signal generator. By using the device, the basalt fibers can be quickly cleaned, etched, treated by a coupling agent and dried. The system is compact in layout, automatic in adjustment, convenient and fast, automatic control is achieved according to a set program, experiment steps are greatly simplified, processing time is saved, and processing efficiency and result success rate are improved.

The invention provides a flammable gas explosion system and method in a small-sized pipeline. The system comprises a flammable gas explosion experiment section in the small-sized pipeline; an acetylene driving section used for detonating a non-sensitive mixed gas; a hydrogen bottle, a nitrogen bottle, a carbon monoxide bottle, an oxygen bottle and an acetylene bottle which are used for providing acombustible gas and air components; a vacuum system used for pumping the experiment section and related pipelines to a vacuum state; a gas mixing system for stirring a flammable mixed gas in the experiment section to be uniformly mixed; and supporting power distribution equipment and datum measurement and acquisition equipment. The experimental procedure is as follows: after an experiment systemis pumped to the vacuum state, according to requirements of working conditions, a certain ratio of the flammable mixed gas is poured into the experiment section, ignition is started and data begin tobe recorded, after explosion is completed, a gas is discharged, and the experiment is finished. The explosion system provided by the invention adopts acetylene and oxygen as detonating gases, and hashigh safety; and simulated gas components are more, and the flammable gas components in a containment after a serious accident of a nuclear reactor happens are more realistically simulated.

The invention provides a preparation method of a catalyst based on a phenolic resin nano material with a hollow shell structure. The catalyst can be well applied to a visible light photocatalysis hydrogen peroxide production system. A hard template method is adopted, silicon dioxide (SiO2) nanospheres are used as a hard template, and a long-chain surfactant cetyl trimethyl ammonium bromide (CTAB)is used as a dispersing agent. The preparation method comprises the following steps: adding CTAB (cetyltrimethyl ammonium bromide) serving as a dispersing agent into a uniformly dispersed silicon dioxide solution, and coating the outer surface of silicon dioxide with precursor solution macromolecules of phenolic resin through polycondensation under the dispersion action of a surfactant; accordingto the method, the morphology of the phenolic resin nano material can be controlled by adjusting the hydrothermal temperature, the hydrothermal time and the concentration of ammonium bifluoride in alkali etching, and excellent catalytic activity is shown. When the catalyst is applied to a photocatalytic hydrogen peroxide production system, it is found that the catalyst can efficiently catalyze hydrogen peroxide production.

The invention provides a method for preparing 2-amino-5-(N-phenothiazinyl)methylene-1,3,4-thiadiazole. The2-amino-5-(N-phenothiazinyl)methylene-1,3,4-thiadiazole is obtained by the steps of adding N-carboxyl-methylene-phenothiazine, thiosemicarbazide and concentrated hydrochloric acid into a reactor, then refluxing to obtain a reaction mixture, cooling the reaction mixture to a room temperature, regulating a pH value of the reaction mixture to 8-9 by using a sodium hydroxide aqueous solution, then cooling the reaction mixture until solid particles are precipitated, suction pumping the solid particles to obtain a filter cake, washing the filter cake by ice water and carrying out recrystallization. The method is simple in experimental step and post-treatment, high in yield and small in secondary pollution, is an economic and environment-friendly industrial preparation method, and fits to need of industrial production.

The invention provides a method for preparing 2-amino-5-(N-phenothiazinyl)methylene-1,3,4-thiadiazole. The2-amino-5-(N-phenothiazinyl)methylene-1,3,4-thiadiazole is obtained by the steps of adding N-carboxyl-methylene-phenothiazine, thiosemicarbazide and concentrated hydrochloric acid into a reactor, then refluxing to obtain a reaction mixture, cooling the reaction mixture to a room temperature, regulating a pH value of the reaction mixture to 8-9 by using a sodium hydroxide aqueous solution, then cooling the reaction mixture until solid particles are precipitated, suction pumping the solid particles to obtain a filter cake, washing the filter cake by ice water and carrying out recrystallization. The method is simple in experimental step and post-treatment, high in yield and small in secondary pollution, is an economic and environment-friendly industrial preparation method, and fits to need of industrial production.

The invention discloses a method for synthesizing alpha-amino ketone from ketone and imine, and belongs to the technical field of chemical organic synthesis. The method comprises the following steps: adding imine into an organic solvent, sufficiently dissolving so as to obtain an imine solution, further respectively adding ketone, a catalyst tetrabutyl ammonium iodide and an oxidant tert-butyl hydroperoxide into the solution, sealing up a tube opening, heating to be 130 DEG C, stirring for 3 hours, cooling down the system to be the room temperature after the reaction is ended, extracting by using dichloromethane and removing the solvent of on organic phase so as to obtain alpha-amino ketone. By adopting the method, defects in the conventional method for synthesizing alpha-amino ketone by electrophilic amination and nucleophilic amination are overcome, direct amination of alpha-position of a simple ketone compound such as acetone is achieved, a C-N bond is generated through oxidative coupling reaction between a N-H bond and a C-H bond at one step, and the alpha-position of a ketone substrate does not need to be prefunctionalized, so that the experiment steps are simple, the condition is gentle, the reagents are cheap, and the range of the substrate is wide; the method is applicable to industrial production.

The invention discloses an in-vitro immature embryo induction and plant regeneration method of seed lotus. The method comprises the following steps: firstly inoculating an immature embryo onto a lotusembryo culture medium to be cultured for 3 to 4 weeks in a light-proof manner, then transferring the induced emerging lotus embryo tissue onto a lotus embryo culture medium to perform subculture, performing dark culture at the temperature of 24 to 26 DEG C, transferring the subcultured good lotus embryo tissue onto a lotus embryo culture medium to perform light culture, finally transferring the lotus embryo tissue with good growth state and becoming green onto a novel seedling raising culture medium to be cultured, so as to form a lotus test-tube seedling. By adopting the in-vitro immature embryo induction and plant regeneration method of the seed lotus, the problem that the lotus distant hybridization offspring fruiting rate is low can be overcome, the survival rate of the lotus distanthybridization offspring can be increased, and the method has certain application value on improving the lotus inheritance and breeding the varieties.

The invention discloses a method for measuring the temperature of a hydrocarbon flame based on a colored CCD camera. According to the method, the temperature measurement range of a measured object is not limited by a blackbody furnace calibration experiment, and a blackbody furnace is not used. The method comprises the following steps: (1) photographing the hydrocarbon flame, namely setting parameters of the colored CCD camera, and photographing the flame; (2) acquiring a relation curve between the temperature and a specific value of original strengths of two base colors, namely acquiring a relation curve between the temperature and a specific value of the original strengths recorded by channels R, G and B; (3) acquiring R, G and B true color images, namely outputting RAW format images from the colored CCD camera, and finally converting the RAW format images into the R, G and B true color images; (4) acquiring a specific value of the two base colors, namely acquiring the original strengths of the three channels R, G and B from the R, G and B true color images, and calculating the specific value of the original strengths of the optional two base colors; and (5) acquiring the temperature, namely acquiring the temperature of the hydrocarbon flame according to the obtained relation curve between the temperature and the specific value of the original strengths of the two base colors.

The invention relates to a method for preparing modified HEK293T cells, the modified HEK293T cells, a method for preparing drug-loaded exosomes, and drug-loaded exosomes. The method for preparing and transforming HEK293T cells includes the following steps: S1: Plasmid construction: using the pcDNA3.1 expression vector as a plasmid backbone, respectively constructing three plasmids: pcDNA3.1-STEAP3, pcDNA3.1-SDC4, and pcDNA3.1-NadB fragments ; S2: The three plasmids in S1 were transfected into HEK293T cells according to the ratio of 1-3:1-3:1-3, and the stable cell lines were screened to obtain the transformed HEK293T cell lines. By adopting the above technical scheme, due to the synergistic effect of these three plasmids, HEK293T cells that secrete a large amount of exosomes can be transformed, thereby further increasing the amount of exosomes secreted and providing more options for clinical trials.

The invention provides a method for enriching cord blood hematopoietic stem cells by using mesenchymal stem cells cultured in a low-oxygen three-dimensional environment. Comprising the following steps: inoculating mesenchymal stem cells on a cell culture substrate with a micro-topological structure on the surface, and culturing under a low-oxygen condition; umbilical cord blood mononuclear cells are inoculated to mesenchymal stem cells for co-culture, so that hematopoietic stem cells are enriched. According to the method, the CD34 positive hematopoietic stem cells in the cord blood mononuclear cells are enriched by the mesenchymal stem cells in a specific low-oxygen three-dimensional environment, and the hematopoietic stem cells in the cord blood mononuclear cells are directly separated through the interaction between the mesenchymal stem cells and the hematopoietic stem cells. The method is low in cost and simple and convenient in experimental steps, and the dryness and activity of the hematopoietic stem cells can be maintained in vitro.

The invention relates to a method for high-efficiency detection of rhodamine B in food and a rapid detection kit. The detection method is mainly divided into three steps: 1, extracting rhodamine B in food; 2, purifying and enriching the rhodamine B in food; and 3, detecting through a machine. The corresponding kit consists of an extracting agent 1, an extracting agent 2 and a solid extraction column 3. When the rhodamine B in food is detected by employing the method, the detection limit can reach 0.5ppb, and the external standard recovery rate is over 85 percent. The food is subjected to pretreatment by employing the method and the key reagents such as the extracting agent 1 and the extracting agent 2, so that the rhodamine B in food is completely extracted, the toxicity of the used reagent is obviously reduced, the required time for detecting the rhodamine B in food is greatly shortened, the detection precision is improved, and the method has the excellent characteristics of high convenience, rapidness, environmental friendliness and high applicability and implementability, and a toxic agent is avoided.

The invention provides a high-efficiency recombined adenovirus containing an ndrg2 gene and the pharmic purpose thereof. The recombined adenovirus contains the ndrg2 gene, a Gateway system and a Virapower Adenoviral Expression system are utilized in the construction process, and the adenovirus is packed by a recombination method in vitro. After being cloned to an introduction carrier, a target gene can be efficiently and quickly cloned to other carriers of a receptor by specific recombination sites on the carriers and recombinase without depending on a restriction enzyme. The package of the adenovirus in vitro simplifies the experiment steps, shortens the operation time, and has high multiplication efficiency, high transgene efficiency and high virus yield efficiency as well as the transduction efficiency as high as 100 percent. The invention solves the technical problem of recombination and package of the ndrg2 gene in vitro as well as probes and determines the lethal effect of the high-efficiency recombined adenovirus to brain glioma cells and cells of lung cancer, liver cancer, gastric cancer and colon cancer. The high-efficiency recombined adenovirus can be used as a medicament for treating brain glioma, long cancer, liver cancer, gastric cancer and colon cancer.