Method for high-efficiency detection of rhodamine B in food and rapid detection kit

A technology for detection kits and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of toxicity, increased detection workload, application restrictions, etc., and achieve simple experimental steps, save detection costs, and improve accuracy Effect

Inactive Publication Date: 2014-12-03
FUJIAN LANHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

my country's entry-exit inspection and quarantine industry standard "Detection method for rhodamine B in imported and exported food" promulgated in 2010 uses liquid chromatography and liquid chromatography-mass spectrometry / mass spectrometry. However, because the pretreatment method is too cumbersome in actual operation, especially the use of GPC purification, it not only increases the workload of detection, but also the efficiency is not very significant, and the use of organic solvents, such as Cyclohexane is both poisonous and harmful. A large amount of use will seriously affect the environment and is not conducive to environmental protection. Due to its own reasons, this method has limited its application in a large number of testing institutions
Recently, there are other methods. It has been reported that ELISA can be used to detect rhodamine B in food. Although this method can detect a large amount of rhodamine B in food, its accuracy and sensitivity are not as good as HPLC. Chromatography, liquid chromatography tandem mass spectrometry, and gas chromatography

Method used

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  • Method for high-efficiency detection of rhodamine B in food and rapid detection kit
  • Method for high-efficiency detection of rhodamine B in food and rapid detection kit
  • Method for high-efficiency detection of rhodamine B in food and rapid detection kit

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Experimental program
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Effect test

Embodiment 1

[0036] Accurately weigh 5 g of crushed chili powder, put it into a 50 mL plastic centrifuge tube with a stopper, add rhodamine B by external addition method, the concentration is 14.4 ng / mL (that is, add 14.4 ng of rhodamine B per mL of extract 1 Rhodamine B), then add 20ml of extractant 1 of the present invention, homogenize at 8000 rpm for 2 minutes, add 5g of extractant 2, shake for 1 minute, and centrifuge at 4500 rpm for 10 minutes; use a pipette to accurately take 5 mL of supernatant , diluted with 10 mL of distilled water to prepare 15 mL of sample solution for later use; pass 15 mL of sample solution through a solid-phase extraction column (LHSPE), vacuumize, and rinse with 5 mL of 20% acetonitrile / water solution; 2 mL of 2% glacial acetic acid / Elute with acetonitrile, add 0.5 mL of 0.1mol / L ammonium acetate solution, and dilute to 2.5 mL; use filter membrane to filter, use high-performance liquid chromatography or high-performance liquid chromatography-mass spectromet...

Embodiment 2

[0043]Accurately weigh 5 g of mashed cured fish, pack into a 50 mL plastic centrifuge tube with a stopper, add rhodamine B by external addition method, the concentration is 14.4 ng / mL, then add 20 ml of extractant 1 of the present invention, Homogenize at 8000 rpm for 2 min, add 10 g of extractant 2, shake for 1 min, and centrifuge at 4500 rpm for 10 min; accurately take 5 mL of supernatant with a pipette, dilute with 10 mL of distilled water, and prepare 15 mL of sample solution for later use ; Pass 15 mL of the sample solution through a solid-phase extraction column (LHSPE), vacuumize, rinse with 5 mL of 20% acetonitrile / water solution; elute with 2 mL of 2% glacial acetic acid / acetonitrile, add 0.5 mL of 0.1mol / L ammonium acetate solution, Dilute to 2.5 mL; use filter membrane to filter, use high-performance liquid chromatography or high-performance liquid chromatography-mass spectrometry (LC-MS) technology to detect, test on the machine, chromatographic conditions: C18 colu...

Embodiment 3

[0049] Accurately weigh 5 g of strawberry jam, put it into a 50 mL plastic centrifuge tube with a stopper, add Rhodamine B by means of external addition, the concentration is 14.4 ng / mL, then add 20 ml of the extractant 1 of the present invention, at 8000 rpm Homogenize for 2 min, add 7 g of extractant 2, shake for 1 min, and centrifuge at 4500 rpm for 10 min; use a pipette to accurately take 5 mL of supernatant, dilute with 10 mL of distilled water, and prepare 15 mL of sample solution, and set aside; After passing through a solid-phase extraction column (LHSPE), vacuumize the mL sample solution, elute with 5 mL 20% acetonitrile / water solution; elute with 2 mL 2% glacial acetic acid / acetonitrile, add 0.5 mL 0.1mol / L ammonium acetate solution, and dilute to 2.5 mL; filtered by membrane filter, detected by high performance liquid chromatography or high performance liquid chromatography-mass spectrometry (LC-MS), tested on the machine, chromatographic conditions: C18 column, 150 ...

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Abstract

The invention relates to a method for high-efficiency detection of rhodamine B in food and a rapid detection kit. The detection method is mainly divided into three steps: 1, extracting rhodamine B in food; 2, purifying and enriching the rhodamine B in food; and 3, detecting through a machine. The corresponding kit consists of an extracting agent 1, an extracting agent 2 and a solid extraction column 3. When the rhodamine B in food is detected by employing the method, the detection limit can reach 0.5ppb, and the external standard recovery rate is over 85 percent. The food is subjected to pretreatment by employing the method and the key reagents such as the extracting agent 1 and the extracting agent 2, so that the rhodamine B in food is completely extracted, the toxicity of the used reagent is obviously reduced, the required time for detecting the rhodamine B in food is greatly shortened, the detection precision is improved, and the method has the excellent characteristics of high convenience, rapidness, environmental friendliness and high applicability and implementability, and a toxic agent is avoided.

Description

technical field [0001] The invention specifically relates to a high-efficiency detection method and a rapid detection kit for rhodamine B in food. Background technique [0002] Rhodamine B, also known as rose bengal B, commonly known as flower pink, is a basic fluorescent dye. As a fluorescent reagent, it has been widely used in the fields of mining, environmental protection, and steel, and is a commonly used reagent for fluorescence analysis. However, some foods have been found to contain rhodamine B dye. Due to its potential carcinogenicity and mutagenicity, countries such as my country and the European Union do not allow it to be added to food. Therefore, the detection of Rhodamine B in food is extremely important and imperative. [0003] The detection of rhodamine B in food mostly adopts high performance liquid chromatography, liquid chromatography tandem mass spectrometry and gas chromatography, but no more efficient and rapid method has been reported so far. my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 李昊
Owner FUJIAN LANHAO BIOTECH
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