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SNP (Single Nucleotide Polymorphism) marker and detection primer related to ENU (Ethyl Nitroso Urea) mutagenesis grass carp family growth characteristics

A grass carp, labeling technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of twisted notochord, elongated anterior-posterior axis of embryos, and undifferentiated somites

Inactive Publication Date: 2019-02-15
SHANGHAI OCEAN UNIV
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Problems solved by technology

And the injection of mstn1 mRNA overexpression can lead to the elongation of the anterior-posterior axis, the shortening of the dorsal-ventral axis, the slight twisting of the notochord, and the strong inhibition of somite development and non-differentiation of zebrafish embryos during somitogenesis.

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  • SNP (Single Nucleotide Polymorphism) marker and detection primer related to ENU (Ethyl Nitroso Urea) mutagenesis grass carp family growth characteristics
  • SNP (Single Nucleotide Polymorphism) marker and detection primer related to ENU (Ethyl Nitroso Urea) mutagenesis grass carp family growth characteristics
  • SNP (Single Nucleotide Polymorphism) marker and detection primer related to ENU (Ethyl Nitroso Urea) mutagenesis grass carp family growth characteristics

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Embodiment Construction

[0077] In order to better explain the technical solutions of the present invention, the embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

[0078] 1 Materials and methods

[0079] 1.1 Test materials and management

[0080] The parents of grass carp induced by ENU were from the Bream Breeding Center of the Ministry of Agriculture of Shanghai Ocean University. The specific mutagenesis steps of ENU mutagenesis grass carp parents are:

[0081]Well-developed 6-year-old grass carp broodstock, 4 females and 4 males, were selected from...

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Abstract

The invention discloses a grass carp SNP (Single Nucleotide Polymorphism) marker. The grass carp SNP marker is any one, two, three or four of a grass carp mstn1 gene 465ntC / G, a grass carp mstn1 gene467nt G / A, a grass carp mstn2 gene 909nt C / T and a grass carp mstn2 gene 1024nt G / A. The mstn1 gene has a GenBank login number of KM874826.1. The mstn2 gene has a GenBank login number of KM874827.1. The invention further discloses a method for finding and detecting the SNP, a primer group and application. The SNP maker can be used for detecting or monitoring weight, whole length, body length, bodyheight, head length, tail length, tail height and body thicknesses of a grass carp, or a combination of any data, or a ratio of any two values.

Description

technical field [0001] The invention belongs to the field of aquatic science and relates to a SNP marker associated with the growth characteristics of ENU mutagenic grass carp family. Background technique [0002] ENU (N-ethyl-N-nitrosourea) is a commonly used chemical mutagen, which can produce alkylation modifications on genomic DNA bases, which can cause DNA mismatches during replication and lead to mutants [1,2] . ENU mainly induces single-base mutations without any tendency, so that the genes are mutated, and its mutagenesis mode is similar to natural variation [3,4] . At the same time, the efficiency of ENU mutagenesis is very high, which is about 10 times that of other mutagenesis methods. In model organisms, using ENU to obtain large-scale mutants for functional gene research has become a common method. However, there are few reports on its application in farmed fish. At present, only the former Soviet Union has reported the use of ENU mutagenesis combined with ...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 邹曙明王成龙郑国栋陈杰周春雪徐文迪
Owner SHANGHAI OCEAN UNIV
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