Cell suspension subpackaging device

A cell suspension and subpackaging technology, applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of easy aggregation of cells, large differences in cell specifications, and high cost of use, and achieve guaranteed The effect of seed cell quality, shortened time required, and large working space

CN109370877APending Publication Date: 2019-02-22章毅 +7
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-02-22

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Abstract

A cell suspension subpackaging device comprises a subpackaging test piece, a blowing test piece, a connecting test piece and a container, wherein the container comprises an accommodating cavity and acover body; the subpackaging test piece, the blowing test piece and the connecting test piece are connected with the cover body respectively. The cell suspension subpackaging device is applicable to quantitative subpackaging operation of various types of cells in a cell factory, achieves two processes of uniform cell mixing and continuous quantitative subpackaging at the same time, greatly shortens the subpackaging time before cell cryopreservation, and reduces contamination risks and cell viability reduction caused by slow cell subpackaging and long exposure time. The cell suspension subpackaging device is simple in structure, accurate in quantitation and easy and convenient to operate.
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Description

technical field

[0001] The invention relates to a device for distributing substances, in particular to a dispensing device for quantitative dispensing of cell suspension to facilitate cell freezing and storage, especially for rapid quantitative dispensing of cells harvested from cell factories. Background technique

[0002] Cryopreservation of cells is currently recognized and widely used as a cell preservation method suitable for maintaining the morphology and functional properties of various types of cells. In addition to the cooling curve during the cryopreservation process and the choice of cryoprotectant will directly affect the effect of cell cryopreservation, pre-cryopreservation treatment is also the key to affecting the quality of cells after thawing, which is especially significant in large-scale cell cryopreservation. Taking a 10-layer cell factory as an example, when the cell confluence reaches 85% to 90%, it contains about 10×10 7 cells ~ 15×10 7 cells, if con...

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Embodiment Construction

[0032] The present invention is further described below in conjunction with accompanying drawing:

[0033] like figure 1 Shown present embodiment dispensing device, by Figure 2 ~ Figure 6 The main test piece units shown are composed.

[0034] The installation composition of the dispensing device of the present embodiment, at first quantitative scale 1 and piston 3 and its pipeline such as figure 2 As shown, it is installed on the bottle cap 9, the dropper 4 is fastened with the bracket on the bottle cap, and the hose 6 is as figure 2 connected to the pipe as shown, blowpipe 5 as figure 2 Assembled from top to bottom as shown. The positions of the test piece and the bottle cap 9 are relatively fixed.

[0035] The working principle of the sub-packaging device of the present invention:

[0036] The first sealing member 11 and the second sealing member 12 move up and down with the piston, so that a certain amount of sample is sucked or flowed out. When the piston moves ...