Cell suspension subpackaging device

Abstract

A cell suspension subpackaging device comprises a subpackaging test piece, a blowing test piece, a connecting test piece and a container, wherein the container comprises an accommodating cavity and acover body; the subpackaging test piece, the blowing test piece and the connecting test piece are connected with the cover body respectively. The cell suspension subpackaging device is applicable to quantitative subpackaging operation of various types of cells in a cell factory, achieves two processes of uniform cell mixing and continuous quantitative subpackaging at the same time, greatly shortens the subpackaging time before cell cryopreservation, and reduces contamination risks and cell viability reduction caused by slow cell subpackaging and long exposure time. The cell suspension subpackaging device is simple in structure, accurate in quantitation and easy and convenient to operate.

Description

technical field [0001] The invention relates to a device for distributing substances, in particular to a dispensing device for quantitative dispensing of cell suspension to facilitate cell freezing and storage, especially for rapid quantitative dispensing of cells harvested from cell factories. Background technique [0002] Cryopreservation of cells is currently recognized and widely used as a cell preservation method suitable for maintaining the morphology and functional properties of various types of cells. In addition to the cooling curve during the cryopreservation process and the choice of cryoprotectant will directly affect the effect of cell cryopreservation, pre-cryopreservation treatment is also the key to affecting the quality of cells after thawing, which is especially significant in large-scale cell cryopreservation. Taking a 10-layer cell factory as an example, when the cell confluence reaches 85% to 90%, it contains about 10×10 7 cells ~ 15×10 7 cells, if con...

AI Technical Summary

Problems solved by technology

At present, pipettes or pipettes are commonly used for cell subpackaging before cryopreservation. The advantage of pipette subpackaging is that it is quantitatively accurate, but the disadvantage is that the local blowing force is too large, and repeated blowing is easy to damage the cells. Scale cell suspension by pipetting evenly

The pipette is relatively easier to pipette the suspension evenly, but the quantification is not accurate, which will cause large differences in cell specifications between different frozen storage batches

In addition, the existing methods require the operator to intermittently blow and blow the cell suspension during cryopreservation and aliquoting

Therefore, the quantitative aliquoting of large-scale cells is the most time-consuming and labor-intensive link in the cryopreservation process, and there are two major technical difficulties that will have a great impact on the activity of cells after cryopreservation: First, the existing methods are difficult to pipette the cells evenly The cells are easy to condense into a single cell, which is not conducive to the protective effect of the cryopreservation agent in the later stage; second, the subpackaging takes a long time, and the cells stand for a long time, which is easy to repeatedly condense, causing the frozen cells to produce flocculent precipitates after recovery. Problems such as high dead cell rate affect the quality of seed cells and their downstream research and clinical applications

[0003] CN108441407A discloses an automatic cell cryopreservation device, which uses an injection push device combined with weighing and automatic heat sealing to realize cell subpackaging, which simplifies the operation steps of experimenters, but the device has a complex structure, difficult cleaning and sterilization of cell pipelines, and high cost of use; The relatively simple quantitative dispensing device fails to solve the problem of agglomeration during the static process of cells, so it is not suitable for quantitative dispensing before cell freezing

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