The invention relates the technical field of 
microorganism culture medium subpackage equipment, in particular to a manual and automatic dual-purpose type 
microorganism culture medium 
thermal insulation subpackage device. The device comprises a subpackage frame, a subpackage hopper, a subpackage hose, and a hose 
water stop assembly, wherein the subpackage frame is provided with the subpackage hopper for containing the 
microorganism culture medium. The device provided by the invention is reasonable and compact in structure, convenient to use, and low in cost; the pressure head of the hose is pulled to move to the right through a draft gear, a subpackage passage is quickly started while the 
extrusion to the hose is relieved, and the subpackage time is shortened; the draft gear is electrically connected with a 
control circuit, the subpackage can be controlled by one hand, scatter can be avoided, and the subpackage is more convenient and fast; or the automatic and quantitative subpackage can be realized through a time controller, and the subpackage is more uniform and accurate; according to a subpackage mode combining the manual operation and automatic operation, the operation is flexible, the accuracy is high, and the subpackage efficiency is improved; the outside of the subpackage hopper is provided with a heating sleeve, solidification and 
caking of the microorganism culture medium are avoided, the subpackage passage can be unblocked, and the subpackage operation can be smoothly completed.