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Method for quickly determining nitrate content in fruits and vegetables

A technology for rapid determination of nitrate content, applied in the field of inspection and detection, can solve the problems of weak nitrate monitoring, high toxicity of reagents, and difficulty in control, and achieve the effect of daily monitoring, low detection cost and short time required

Inactive Publication Date: 2019-03-01
徐欣婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of convenient and effective detection methods for vegetable nitrate, the current domestic monitoring of nitrate is still quite weak
[0004] Most of the detection methods currently on the market need to be equipped with expensive instruments and equipment, and are complicated and time-consuming, which cannot meet the needs of on-site detection.
Although the phenol disulfonic acid colorimetric method used in the national standard is more sensitive, the operation steps are complicated, and the intermediate process requires anhydrous, which is difficult to control and takes a long time to complete
Another kind of naphthalene ethylenediamine hydrochloride colorimetric method, nitrite interferes seriously, must be reduced with metal cadmium or zinc, the operation is complicated, and the reagent toxicity is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The rapid determination method for nitrate content in fruits and vegetables of the present invention comprises the following steps: taking fruit and vegetable samples and crushing, weighing 2 g of homogenate in a 250mL volumetric flask, adding water to shake to constant volume, filtering with filter paper, and reusing the filtrate with a filter membrane Filter to obtain a colorless and transparent solution, take the transparent filtrate to measure its absorbance value at 210 nanometers, and calculate the nitrate content in the fruit and vegetable samples through the nitrate standard curve.

[0014] According to the rapid determination method for nitrate content in fruits and vegetables of the present invention, the filter membrane adopts a filter membrane of 0.2 microns.

[0015] According to the rapid determination method for nitrate content in fruits and vegetables of the present invention, a homogenizer is used for crushing the samples of fruits and vegetables.

Embodiment 2

[0017] On the basis of Example 1, the rapid assay method for nitrate content in fruits and vegetables of the present invention comprises the steps of: taking fruit and vegetable samples and pulverizing them, weighing 10 g of the homogenate in a 250 mL volumetric flask, adding water to shake to constant volume, and using filter paper Filtrate the filtrate with a filter membrane to obtain a colorless and transparent solution, take the transparent filtrate to measure its absorbance value at 210 nanometers, and calculate the nitrate content in the fruit and vegetable samples through the nitrate standard curve.

[0018] According to the rapid determination method for nitrate content in fruits and vegetables of the present invention, the filter membrane adopts a filter membrane of 0.2 microns.

[0019] According to the rapid determination method for nitrate content in fruits and vegetables of the present invention, a homogenizer is used for crushing the samples of fruits and vegetabl...

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Abstract

The invention discloses a method for quickly determining the nitrate content in fruits and vegetables and belongs to the field of inspection and detection. The method is characterized in that fruit and vegetable samples are smashed, water is added, volume is fixed after shaking, filtration is performed with filter paper to obtain a colorless and transparent solution, transparent filtrate is obtained, the absorbance value of the filtrate is determined, and the nitrate content in the fruit and vegetable samples is calculated according to a nitrate standard curve. The operation is simple, almostchemical agent is not needed, only one portable spectrophotometer needed to be arranged, the detection cost is low, the required time is short, the demands for on-spot quick detection of vegetable production and sell enterprises can be met, accordingly daily monitoring of the nitrate content is achieved, and timely adjustment of fertilization modes and guidance of healthy consumption of consumersare facilitated.

Description

technical field [0001] The invention belongs to the field of inspection and detection, and in particular relates to a rapid determination method for nitrate content in fruits and vegetables. Background technique [0002] Nitrate is widely found in the human environment and is the most prevalent nitrogen-containing compound in nature. Modern medicine has proved that nitrate in humans and animals can be reduced to nitrite by bacteria. If nitrite accumulates too much, on the one hand, it can directly cause hypoxia poisoning in humans and animals, resulting in methemoglobin. On the other hand, under acidic conditions, , nitrite can combine with secondary amines to form nitrosamines. Among the more than 120 nitrosamine compounds that have been discovered, about 75% of the nitrosamines can induce cancer in the digestive system. Vegetables are a kind of plant food that is easy to accumulate nitrates. More than 80% of the nitrates ingested by the human body come from vegetables. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 徐欣婷
Owner 徐欣婷
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