Small-molecule synthetic compound 1712 anti-ebola virus activity screening method

A compound and small molecule technology, which is applied in the field of activity screening of small molecule synthetic compound 1712 against ebola virus, can solve the problems of screening method of small molecule synthetic compound 1712 against ebola virus and the lack of specific reports on the use method, and achieves credible results. high degree of effect

Active Publication Date: 2019-03-19
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0005] In view of the deficiencies in the prior art, the present invention provides a small molecule synthetic compound 1712 for anti-ebola virus activity screening, which solves the problem of the authoritativeness of literature reports, which still needs to be verified through rigorous experiments, but regarding the small molecule synthetic compound 1712 There are no specific reports on the screening method and application method of anti-ebola virus activity

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  • Small-molecule synthetic compound 1712 anti-ebola virus activity screening method
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  • Small-molecule synthetic compound 1712 anti-ebola virus activity screening method

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] Such as Figure 1-3 As shown, the present invention provides a technical scheme: a kind of activity screening of small molecule synthetic compound 1712 anti-ebola virus, and its steps are as follows:

[0032] S1: Culture of human embryonic kidney cells (293T), human lung epithelial cells (A549) and HeLa cells, the human embryonic kidney cells (293T), human lung epithelial cells (A549) and HeLa cells need to be selected Human embryonic kidney cells (293...

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Abstract

The invention discloses a small-molecule synthetic compound 1712 anti-ebola virus activity screening method, and relates to the technical field of antiviral drugs. The small-molecule synthetic compound 1712 anti-ebola virus activity screening method includes the step: culturing human embryonic kidney cells (293T), human pulmonary epithelial cells (A549) and HeLa cells. A complete screening systemis formed by cell culture, pseudovirus preparation, small-molecule compound preparation, anti-EBOV pseudovirus activity screening, preliminary action mechanism research, infectious EBOV experiments and experimental result analysis, all links of the screening system relate to specific experimental steps and comparative verification of experimental results, the results are high in reliability, related operation steps are described in all the links, and scientificity of the screening mode and method is further embodied.

Description

technical field [0001] The invention relates to the technical field of antiviral drugs, in particular to the activity screening of a small molecule synthetic compound 1712 against ebola virus. Background technique [0002] Ebola virus is transmitted through bodily fluids. Mucus, saliva or blood are all mediums. It can even be transmitted by shaking hands. Ebola is a deadly bloody fever infection that can infect humans, monkeys and chimpanzees. In primates, more than half of human patients bleed to death within two weeks. [0003] At present, there have been many literature reports on the study of small molecule compounds in different cycles of EBOV replication infection, and the molecular types are various, such as the compound LJ001 that inhibits the early stage of EBOV replication infection, and the cathepsin CatB / L inhibitor, all of which belong to amide derivative compounds , Shah et al. reported in 2010 that using a compound called tetrahydroquinoline oxidized carbamat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 崔清华荣立军杜瑞坤
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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