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A kind of rapid propagation method of Kingsberry tissue culture

A tissue culture and goldenseal technology, applied in the field of plant tissue culture, can solve problems such as environmental pollution and operator safety threats, and achieve the effects of high induction rate, cost saving and good plant growth.

Active Publication Date: 2022-03-22
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of mercuric acid in the test will pose a threat to the safety of operators on the one hand, and pollute the environment on the other hand. At present, the use of mercuric acid as a disinfectant has been banned in China

Method used

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  • A kind of rapid propagation method of Kingsberry tissue culture
  • A kind of rapid propagation method of Kingsberry tissue culture
  • A kind of rapid propagation method of Kingsberry tissue culture

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0027] The rapid propagation method of Kingsberry tissue culture of the present invention, its preferred embodiment is:

[0028] The tissue culture and rapid propagation is carried out with the stem section of the golden plum as the explant, including the steps:

[0029] Explant disinfection treatment:

[0030] First clean the outside of the branches with a brush in detergent water, and rinse with running water for 2-3 hours, then, disinfect and sterilize the surface of the explants on an ultra-clean workbench, soak in 75% alcohol for 30 seconds, and rinse with sterile water for 1 hour. -2 times, soak in 5% sodium hypochlorite for 7-8 minutes, rinse with sterile water for 3 times, put on sterile filter paper to dry for inoculation;

[0031] Primary culture:

[0032] The medium formula suitable for the primary culture of King Slime is MS+6-BA 2.0mg / L+NAA 0.1mg / L;

[0033] Subculture Proliferation:

[0034] The most suitable medium for subculture and multiplication of Golden...

specific Embodiment

[0046] 1 material

[0047] Stem segments with dormant buds of Hypericum patulum were obtained from Dali, Yunnan.

[0048] 2 methods

[0049] (1) Disinfection treatment of explants

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Abstract

The invention discloses a method for rapid propagation by tissue culture of Kingsberry. The stem section of Kingsberry is used as an explant for tissue culture and rapid propagation, and the surface of the explant is sterilized by soaking in 5% sodium hypochlorite; the medium formula for primary culture It is MS+6‑BA 2.0mg / L+NAA 0.1mg / L; the subculture medium is MS+6‑BA 1.0mg / L+NAA 0.1mg / L+GA 3 0.1mg / L; the medium for rooting is 1 / 2MS+IBA 0.5mg / L+activated carbon 0.1g / L; before transplanting, remove the rooted seedlings from the culture bottle and wash the sticky particles on the roots with 2% carbendazim solution The culture medium was planted in the prepared peat soil:perlite=2:1 mixed matrix container. The harm to the environment is small, the induction rate is high, and the cost is saved; the plants grow well after transplanting, and the survival rate after transplanting is 100%.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for rapid propagation of hypericum plum tissue culture. Background technique [0002] As an indispensable means and important part of modern plant biotechnology, plant tissue culture refers to the cultivation of plant tissues, organs, embryos, cells, and protoplasts under sterile conditions, through artificial control, and using artificial media. The process of in vitro culture to make it develop into a complete plant. [0003] Hypericum patulum, also known as Forsythia yunnanensis and Eargrass flower, is a semi-evergreen shrub of the genus Hypericum in the family Guttiferae. 0.3-1.5 meters high, the stem is light red to orange, the flower buds are broad and ovoid, the petals are golden yellow, no red halo, somewhat incurved, oblong obovate to wide obovate, many stamens, the flowering period is June-July, most of its varieties have Golden flowers and bright fruit co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 李潞滨刘蕾许丽君游胜利
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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