The invention discloses a method for breeding, culturing and preserving paratylenchus by using carrot callus, belonging to the field of plant-parasitic nematode culture. The method comprises the following steps of: (1) under a sterile condition, soaking carrots with alcohol, scorching, peeling and slicing the carrots, putting carrot slices into a sterilized culture dish, sealing an opening of the sterilized culture dish, putting into a culture box, and culturing till the carrot slices grow callus; (2) sterilizing the paratylenchus with a streptomycin sulfate solution, centrifuging and collecting, eluting with sterile water, centrifuging and collecting again, preparing a paratylenchus suspension with sterile water, and dripping the suspension on the carrotcallus; and (3) putting the carrot callus inoculated with the paratylenchus into the culture box, and culturing. In the method, a culture medium is not required for culturing, so that the operation is simple and convenient; and sterilization of the streptomycin sulfate solution does not affect the activity of the paratylenchus, so that the aim of breading the paratylenchus simply, efficiently and largely is achieved, the paratylenchus can be preserved for a long time, and parasitism and pathogenicity of the paratylenchus on a plant are kept.