The invention relates to a reagent storage box for gene sequence detection, which includes a box body, a box cover, a thermal insulation cushion, an irradiation inactivation lamp, an elastic inner lining, a semiconductor refrigeration device, a silica gel heating wire, a driving power supply, a temperature sensor, a photovoltaic Power generation board and control circuit, the box cover and the upper surface of the box body are hinged, the insulation cushion is embedded on the inner surface of the box body and the box cover, the radiation inactivation lamp and the temperature sensor are evenly distributed on the inner surface of the box cover, and the elastic inner lining layer Embedded in the box body, a number of test tube holes are evenly distributed in the elastic lining layer, the silicone heating wire is embedded in the upper end surface of the bottom of the box body, at least one control chamber is set at the bottom of the box body, and the semiconductor refrigeration device, driving power supply and control circuit are all embedded in the control chamber Inside. On the one hand, the present invention has a good carrying capacity, which can effectively meet the needs of various reagent storage and transfer operations at the same time; on the other hand, it can effectively adjust the temperature of the storage environment during the reagent storage process, and has a certain self-power supply capacity , effectively reducing energy consumption.