Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of detection method of lactic acid bacteria in probiotics

A detection method and technology of lactic acid bacteria, which can be applied in measuring devices, immunoassays, and biological material analysis, etc., can solve the problems of decreased biological activity of lactic acid bacteria, low detection rate of probiotics, and long cultivation time, etc., so as to improve the detection rate and prevent Insufficient or excessive biotinylation, the effect of reducing interference

Active Publication Date: 2022-01-28
HENAN BUSINESS SCI RES INST
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional national standard detection method takes at least 3 days to produce results, and the cultivation time is long, time-consuming and laborious; in addition, in the actual detection process, the detection environment is not conducive to the growth of bacteria and the long detection time and other factors make the biological activity of lactic acid bacteria decline or even In the case of death, the detection rate of probiotics in the final product is lower than that in the actual sample

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of detection method of lactic acid bacteria in probiotics
  • A kind of detection method of lactic acid bacteria in probiotics
  • A kind of detection method of lactic acid bacteria in probiotics

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Preparation of Lactic Acid Bacteria Immunomagnetic Beads

[0029] a) Dilute the lactic acid bacteria antibody to 1mg / mL with a pH value of 7.4 and a PBST buffer solution with a concentration of 0.01mol / L. The lactic acid bacteria antibody is Bifidobacterium antibody, Streptococcus thermophilus antibody and Lactobacillus antibody in a weight ratio of 4 : 3:3 mixed mixture, prepare 10mg / mL biotin N-hydroxysuccinimide ester solution with anhydrous DMSO, add diluted lactic acid bacteria antibody to the biotin N-hydroxysuccinimide ester solution Mix, the volume ratio of biotin N-hydroxysuccinimide ester solution to lactic acid bacteria antibody is 4:1, and incubate at room temperature to obtain biotin-labeled lactic acid bacteria antibody;

[0030] b) Add 1 mg of activated and dissolved streptavidin-modified magnetic beads to the biotin-labeled lactic acid bacteria antibody for mixing to obtain a mixture of antibody and magnetic beads, and place at room temperature;

[0031...

Embodiment 1

[0032] The selection of embodiment 1 BSAV concentration

[0033] Take 6 parts of 225mL PBS buffer containing different concentrations of BASV (PBS concentration is 0.02mol / L) in homogeneous bags, and add 25g of bifidobacteria, lactobacilli and Streptococcus thermophilus to each homogeneous bag The positive samples were homogenized for 30 seconds. After homogeneous mixing, the sample homogeneous solution was obtained. Since the concentration of the target bacteria in the sample homogeneous solution was high at this time, it could not be counted directly according to the detection method specified in GB4789.35, so the sample needs to be Dilute the homogeneous solution, and dilute the sample homogeneous solution to 10 times according to the 10-fold gradient dilution. -5 , to obtain diluted sample homogenate; the concentrations of BSAV added were 0% (no BSAV added), 0.5%, 1%, 2%, 3% and 5%.

[0034] In addition, take 225mL 0.85% normal saline as the contrast solution, add 25g of ...

Embodiment 2

[0038] Embodiment 2 The selection of lactose solution concentration

[0039] Take 25g of 7 positive samples containing Bifidobacterium, Lactobacillus and Streptococcus thermophilus, add 225mL of PBS buffer solution containing 1% BASV into the homogenization bag, homogenize for 30s, after homogenization, mix according to 10 times Serial dilution, diluted to 10 -5 , to obtain the diluted sample homogeneous solution, add 20mL of lactose solution to the diluted sample homogeneous solution, the concentration of lactose solution is 0% (national standard), 1%, 2%, 5%, 8%, 10% and 20% respectively. %.

[0040]In accordance with the detection method specified in GB4789.35, the above sample liquid was subjected to operations such as enrichment, scribing and separation, and plate counting to obtain the test results. The results are shown in Table 2.

[0041]

[0042] From the results in Table 2, it can be seen that the number of colonies that can be detected is the highest after add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting lactic acid bacteria in probiotics, and belongs to the technical field of detection of microbial products. The method includes: (1) adding the sample to be tested into PBS buffer solution added with BSAV, homogenizing to prepare a sample homogeneous solution, the dosage ratio of the sample to be tested and PBS buffer solution is 1g:9mL; Lactose solution was added to the solution, and the supernatant was removed by water bath and centrifugation to obtain a bacterial suspension; (2) Lactic acid bacteria immune magnetic beads were added to the bacterial suspension obtained in step (1), mixed and oscillated, and statically placed on a magnetic stand Set the layers, discard the upper layer, and elute the lower layer samples, add PBS buffer solution to the eluted lactic acid bacteria immunomagnetic beads, oscillate and resuspend the magnetic beads, and obtain the sample solution to be tested; (3) incubate the sample to be tested , observation, detection. The invention shortens the detection time of the lactic acid bacteria in the sample, improves the detection rate of the lactic acid bacteria and the accuracy of the detection results, and has high detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of detection of microbial products, and in particular relates to a detection method of lactic acid bacteria in probiotics. Background technique [0002] As a type of active microorganisms that can have beneficial effects on the host's physiological functions, probiotics have the functions of regulating intestinal flora, lowering cholesterol, enhancing immunity, alleviating oral diseases and emotional abnormalities, etc., and are widely used in the fields of food, medicine and Livestock field. In recent years, the research and development, production and sales of lactic acid bacteria and their products in probiotics show that the important functions of lactic acid bacteria in human health have attracted more and more attention from researchers and consumers. Lactic acid bacteria mainly include bifidobacteria , Streptococcus thermophilus and Lactobacillus are more sensitive to ambient temperature. During the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56911G01N2333/335G01N2333/315G01N2333/195G01N2469/10
Inventor 周莉王法云刘红伟关炳峰张立攀李向力平洋张亚勋李远远任钊谭静罗蓓蓓王珂王瑞陈俭春
Owner HENAN BUSINESS SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com