Construction method of cashew regeneration system

A system and technology of cashew nuts, applied in the field of cashew regeneration system construction, can solve the problems of unformed industrialized production mechanism, low natural reproduction rate, low reproduction coefficient, etc., and achieve the effect of promoting the commercialization process

Inactive Publication Date: 2019-04-09
广西玉林玖旺农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, cashew nuts mainly rely on seed propagation for seedling production, which has problems such as long reproduction cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Establishment of a sterile system: Select mature, plump, complete cashew seeds without pests and diseases, and preliminarily wash them under tap water. Seeds were soaked in 0.1% potassium permanganate solution for 4 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 9 minutes, rinsed with sterile water for 5 times, and washed with sterile filter paper. Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.5mg / L 6-BA+0.4mg / L NAA4.5% sucrose+0.4% agar+0.3% activated carbon, the pH value is 5.6.

[0016] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the aseptic system on the primary medium for cultivation. After inoculation, first culture in total darkness at 24°C for 10 days, and then place in light for 15 hours a day...

Embodiment 2

[0022] (1) Establishment of a sterile system: Select mature, plump, complete cashew seeds without pests and diseases, and preliminarily wash them under tap water. Seeds were soaked in 0.3% potassium permanganate solution for 3 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 5 minutes, rinsed with sterile water for 3 times, and washed with sterile filter paper. Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.6mg / L 6-BA+0.5mg / L NAA +3.8% sucrose+0.5% agar+0.1% activated carbon, the pH value is 5.8.

[0023] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the aseptic system on the primary medium for cultivation. After inoculation, first culture in total darkness at 24°C for 13 days, and then place in light for 16 hours a d...

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PUM

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Abstract

The invention discloses a construction method of a cashew regeneration system. Cashew is also known as anacardium occidentalie linn, belongs to anacardiaceae and perennial arbor, is planted in Indonesia, Malaysia, Sri Lanka and India, is further successfully planted in southern provinces in China, is plated over a hundred years by human, and belongs to one of four major dried fruits. The inventionrelates to a tissue culture rapid propagation method of cashew, cashew is taken as an initial explant, a cashew regeneration system is constructed through processes of asepsis system establishment, primary culture, multiple shoot propagation culture, multiple shoot strong seedling culture, test-tube plantlet rooting and test-tube plantlet acclimatizating and transplanting, and a production methodis provided for the rapid propagation of cashew high-quality seed sources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for constructing a cashew regeneration system. Background technique [0002] Cashew is also known as the tree, which belongs to Anacardaceae, perennial tree, up to 12 meters high, with latex. Simple leaves, alternate, oblong-ovate or obovate, leathery, 10-20 cm long, 5-10 cm wide, glabrous. Large panicles, covered with rust-colored hairs. Flowers yellow, containing one ovule. Nut kidney-shaped, compressed with two appendages, about 2.5 cm long, with a hard exocarp containing bitter resin, which contains oily edible seeds; the fruiting stem expands to form a large and fleshy pear-shaped structure (false fruit), 5-9 cm long, bright yellow or red when cooked. The swollen fruit stalks are sweet and sour, edible or brewed, and the nut seeds are fragrant and can be fried, commonly known as cashew nuts; the shell is pressed for oil, which ca...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王小宇王露
Owner 广西玉林玖旺农业科技有限公司
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