Composition and detection method of related substance in pramipexole starting material
A detection method and a technology for related substances are applied in the field of chemical analysis and detection of pharmaceutical impurities to achieve good system applicability, specificity, and convenient control.
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Embodiment 1
[0110] Embodiment 1 uses screening result to carry out impurity detection
[0111] Use high performance liquid chromatography analysis method to detect and comprise the quantitative detection of impurity PMH-SM1-Z1, impurity PMH-SM1-Z3 in starting material PMH-SM1, chromatographic conditions are as follows:
[0112] Chromatographic column: the model is Thermo hypersil Gold Aq, 4.6mm×150mm, 5μm.
[0113] Mobile phase: use phosphate buffer (weigh 4.6g sodium octane sulfonate, 9.1g potassium dihydrogen phosphate, add water 1000ml to dissolve, adjust pH value to 3.0 with phosphoric acid) as mobile phase A, use acetonitrile-mobile phase A volume The ratio of 50:50 is liquidity B;
[0114] Detection wavelength: select wavelength 264nm to detect impurity PMH-SM1-Z1 and other unknown impurities, select wavelength 210nm to detect impurity PMH-SM1-Z3.
[0115] Detection column temperature: 40°C.
[0116] (1) Get an appropriate amount of starting material to be detected, accurately we...
Embodiment 2
[0133] Application of embodiment 2 detection method
[0134] Using the chromatographic conditions and detection method of Example 1, the pramipexole starting material (S)-2,6-diamino-4,5,6,7-tetrahydrobenzothiazole was detected for multiple batches of impurities. The results are shown in the table below.
[0135] Table 1. The first batch of test results
[0136]
[0137] Table 2. The second batch of test results
[0138]
[0139] Table 3. The third batch of test results
[0140]
[0141] The above results illustrate that the detection method of the present invention has stability and repeatability, and can stably detect the pramipexole starting materials of different manufacturers and batches.
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