Tree shrew behavior sensitization model evaluation method and open field experiment device for evaluation

Abstract

The invention discloses a tree shrew behavior sensitization model evaluation method and an open field experiment device for the evaluation, the method comprises the following steps of: inducing the tree shrew behavior sensitization by METH, detecting the tree shrew behavior science, the motion distance, the speed and the track by an open field experiment, measuring the weight change of the tree shrew and marking plate behaviors; the invention also discloses an open field experiment device for evaluating the tree shrew behavior sensitization model, which comprises a box body, a bottom plate replacement box, a moving chassis, a fixing bracket and a camera, wherein the bottom of the moving chassis is provided with universal wheel with a brake, the box body is fixed on the top of the bottom plate replacement box, the bottom plate replacement box is fixed on the moving chassis, an upright post of the fixing bracket is fixed on the moving chassis, and the camera is fixed on the fixing bracket. The device provides reference and guidance for the success of establishing the tree shrew behavior sensitization model, and is convenient for researching the addiction mechanism of lazy drugs by means of the tree shrew behavior sensitization model; the open field experiment device can conveniently replace the bottom plate after each batch of experiments are finished, thereby reducing the time for cleaning, scraping, assembling and other processes.

Description

technical field [0001] The invention relates to a tree shrew behavior sensitization model evaluation method and an open field experimental device used for the evaluation. Background technique [0002] Behavioral sensitization refers to the process in which after repeated and intermittent administration of morphine, methamphetamine (METH), cocaine and other dependent drugs, animals show increased voluntary activities, stereotyped behaviors and other changes in the behavioral effects of dependent drugs. Behavioral sensitization is one of the characteristic manifestations of drug addiction, which is closely related to drug relapse and craving. At present, a large number of researches on drug addiction involve the establishment of animal models of behavioral sensitization. However, most of the experimental animals used to establish these models are rats or mice. Due to the large differences in the nervous system between rodents and humans, the research results often cannot refle...

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Problems solved by technology

[0003] So far, there are few reports on tree shrews used as experimental animals for METH behavioral sensitization research, and through open field experiments to detect changes in tree shrew behavior, movement distance, speed and trajectory, statistical stereotyped behavior scores and record body weight changes, and Establish a METH-induced tree shrew behavior sensitization model; at the same time, the existing open-field experimental device needs to clean the bottom plate after each experiment to eliminate the factors with iconic characteristics such as the odor left by the experimental subjects on the bottom plate, and prevent these factors It will affect the experimental objects, and when the number of experimental objects is large and open field experiments need to be carried out in batches, removing the bottom plate and cleaning it will undoubtedly increase the experimental time, and the cleaning time is too long will undoubtedly affect the drug in the experimental objects. The efficacy of the drug caused great deviations in the experimental results

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