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Preparation method of high-potency positive serum for porcine pseudorabies virus

A technique for porcine pseudorabies virus and porcine pseudorabies, applied in chemical instruments and methods, antiviral immunoglobulin, immunoglobulin from serum, etc., can solve the problem of low titer of positive serum of porcine pseudorabies virus and achieve titer high effect

Inactive Publication Date: 2019-04-16
成都史纪生物制药有限公司
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Problems solved by technology

Indicates that the titers of existing porcine pseudorabies virus positive sera are actually lower

Method used

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  • Preparation method of high-potency positive serum for porcine pseudorabies virus
  • Preparation method of high-potency positive serum for porcine pseudorabies virus
  • Preparation method of high-potency positive serum for porcine pseudorabies virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Example Preparation of Porcine Pseudorabies Virus High Titer Positive Serum

[0029] (1) Preparation of inactivated vaccine

[0030] 1) Preparation of aqueous phase

[0031] The porcine pseudorabies virus solution cultured in suspension was removed by titanium rods and 3 μm filter elements to remove carriers and cell debris to obtain a high-titer and relatively single porcine pseudorabies antigen solution (titer ≥ 10 8.0 TCID 50 / ml), stock solution and BEI (diethyleneimine) were added to freshly prepared BEI according to the volume ratio of 100:1 to obtain the aqueous phase, and then the aqueous phase was placed in a bed shaken at 3000 rpm at 37°C for 48 hours to inactivate Finally, store the aqueous phase at 4°C and wait until it passes the sterility and inactivation tests for later use.

[0032] 2) Preparation of immune enhancer

[0033] In sodium chloride buffer, add 0.4mg / L PolyI:C, 0.05mg / L muramyl dipeptide and 0.5mg / L cimetidine, then add Tween-80, be prepar...

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Abstract

The invention provides a preparation method of high-potency positive serum for a porcine pseudorabies virus. The method takes a rabbit as an immunization object, and immunizes the rabbit with an inactivated vaccine containing a suitable amount of PolyI:C, muramyl dipeptide and cimetidine to produce the hyperimmune serum, can achieve the effect that no cytopathic effect occurs in subculture of at least 2 generations in a T25 square flask equal volume ratio neutralization 1 vaccine experiment, and meets the requirement that a cell method serves as an exogenous virus detection standard in the <Chinese veterinary pharmacopoeia> (2015 edition). The preparation method of the high-potency positive serum for the porcine pseudorabies virus has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological products, in particular to a method for preparing porcine pseudorabies virus high-titer positive serum. Background technique [0002] Hyperimmune serum is made by using a certain vaccine (bacteria) to repeatedly immunize a certain animal according to a certain procedure, and after the antibody titer reaches a certain level, the immunized animal is slaughtered and the whole blood is collected, and then it is made by special treatment. of a biological product. This hyperimmune serum is mainly used for exogenous virus detection of biological products. [0003] Porcine pseudorabies virus hyperimmune serum is mainly used for exogenous virus detection of veterinary biological products. At present, the animal sources for the preparation of hyperimmune serum for porcine pseudorabies virus are mainly pigs and rabbits. As a native animal, pigs can provide a large amount of serum, but the risk of exogenous viruse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/06
CPCC07K16/085C07K2317/10
Inventor 邢刚方芳岳丰雄黄杰王洁清杨勇徐祥兰廖鏖
Owner 成都史纪生物制药有限公司
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