circRNA and application thereof in preparation of cervical cancer diagnostic reagent

A technology for cervical cancer and reagents, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the low incidence of cervical cancer, cervical cancer screening and the heavy burden of primary prevention health economics, etc. problem, to achieve the effect of accurate detection method and high detection accuracy

Active Publication Date: 2019-04-23
上海市长宁区妇幼保健院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, with the widespread application of HPV vaccine, cervical cancer has achieved a certain degree of primary prevention, and its morbidity and mortality have dropped significantly in many developed countries, but the high HPV infection rate is accompanied by relatively low cerv

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  • circRNA and application thereof in preparation of cervical cancer diagnostic reagent
  • circRNA and application thereof in preparation of cervical cancer diagnostic reagent
  • circRNA and application thereof in preparation of cervical cancer diagnostic reagent

Examples

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Effect test

Embodiment 1

[0048] Example 1 Data Screening and Integrated Analysis

[0049] The GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information), and the GEO database is the largest database of gene expression data. Develop dataset inclusion criteria:

[0050] ①The selected data set must be genome-wide mRNA / miRNA / circRNA transcriptome data;

[0051] ② These data come from tissues of cervical squamous cell carcinoma (CSCC) case group and control group (N) (without drug stimulation or transfection);

[0052] ③ This study considers standardized or original data sets.

[0053] After screening, the following data sets were obtained:

[0054] Table 1 List of datasets included in screening

[0055]

[0056] data analysis:

[0057] The tool used was R-3.3.3, and the metaMA package was used for analysis. The method used for p-value merging in meta-analysis was the inversenormal method.

[0058] Screening criteria for differentiall...

Embodiment 2

[0063] Example 2 RT-PCR verification of the relative expression of hsa_circ_0000069 in cervical squamous cell carcinoma tissues and adjacent tissues

[0064] 1. Materials

[0065] Tissue samples from 27 patients with cervical squamous cell carcinoma who underwent surgery in the gynecology ward of Shanghai Changning District Maternal and Child Health Hospital were collected, and the corresponding paracancerous tissues of the patients were used as the control group. The pathological diagnosis of the patients was verified by pathologists. None of the patients had received radiotherapy or chemotherapy before operation, and had no other surgical, endocrine, metabolic, infectious diseases and other gynecological diseases such as uterus and ovary. Fresh tissue specimens were obtained under sterile conditions, washed with normal saline to remove blood, and divided into RNase-free cryopreservation tubes, quick-frozen in liquid nitrogen overnight, and stored in a -80°C refrigerator for...

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Abstract

The invention relates to circRNA and application thereof in preparation of a cervical cancer diagnostic reagent, in particular to application of hsa_circ_0000069 in preparation of a cervical cancer diagnostic reagent. Further mining and integrated analysis are carried out on sequencing data of cervical cancer, a biological information regulation network of the interaction of circRNA-miRNA-mRNA iscreated by means of a biological information method, and the key molecules hsa_circ_0000069 and hsa-miR-125b-5p which are involved in regulation of cervical cancer are searched for. A new molecular diagnostic marker for diagnosis of cervical cancer is provided, and a research basis for gene detection of clinical diseases is offered.

Description

technical field [0001] The invention belongs to the technical field of tumor gene detection, and in particular relates to circRNA and its application in the preparation of cervical cancer diagnostic reagents. Background technique [0002] In recent years, circular RNA (circular RNA, circRNA) has become another non-coding RNA research hotspot after miRNA and 1ncRNA, and has attracted extensive attention from scholars at home and abroad. At present, the research on the biological function of circRNA is still in the exploratory stage. It has been found that circRNA can be used as a competitive endogenous RNA, that is, miRNA sponge, to regulate the expression of target genes, and can also be used as a transcription regulator or RNA binding protein to indirectly regulate genes at the post-transcriptional level. , under certain conditions, circRNA can even directly translate proteins. Studies have shown that the expression level of ciRS-7 in cervical cancer tissue is significantl...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
Inventor 马成斌刘平杨龙涛张文缨吴琼蔚刘彧
Owner 上海市长宁区妇幼保健院
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