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A strain of Beauveria bassiana bbjl-01 with strong pathogenicity to the last instar larva of Cryptomeria caterpillar

A technology of Beauveria bassiana and Beauveria bassiana, which is applied in the field of agricultural biology, can solve the problems of serious damage to the last instar larvae and poor control effect, and achieve good results in comprehensive indicators

Active Publication Date: 2020-07-31
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, the current use of Beauveria bassiana for pest control is mainly aimed at the first and middle instar larvae, and less control of the last instar larvae is mainly due to the strong resistance of the last instar larvae, and there is no suitable biological agent. Its control, or the control effect is not good, but the damage of the last instar larvae is more serious

Method used

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  • A strain of Beauveria bassiana bbjl-01 with strong pathogenicity to the last instar larva of Cryptomeria caterpillar
  • A strain of Beauveria bassiana bbjl-01 with strong pathogenicity to the last instar larva of Cryptomeria caterpillar
  • A strain of Beauveria bassiana bbjl-01 with strong pathogenicity to the last instar larva of Cryptomeria caterpillar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and identification of pathogenic fungi

[0031] 1. Test strains

[0032] The strains used in this experiment were derived from natural worms infected by Beauveria bassiana. The isolated and purified strains were inoculated on slant medium and stored in Fujian Agriculture and Forestry University’s key laboratory for the prevention and control of major pests in ecological public forests in Fujian Province. See Table 1 for sample information.

[0033] Table 1 Sample information

[0034]

[0035] 2. Determination of growth rate and sporulation of different strains

[0036] Different strains were inoculated on the center of the PDA plate by the drop method, cultured in an incubator at 25°C, and the diameter of the colony was measured once a day by the cross method, and three replicates were set for each treatment, and a total of 15 days were observed to compare the colony growth of different strains speed. At the same time, record the time when the...

Embodiment 2

[0055] 1. Toxicity determination

[0056] The isolated Beauveria bassiana strains were respectively transferred to PDA medium and cultured at a constant temperature of 25°C. After 10 days, the spores were collected, and a certain amount of spores were scraped from the plates of each strain, placed in sterilized 0.1% Tween-80 sterile water, and 1.0 × 10 7 For the spore suspension, use 0.1% Tween-80 sterile water as a control. Soak the 7th instar larvae of the cedar caterpillar in the prepared spore suspension for 10 seconds, absorb excess water with filter paper, then put the larvae into a jar with cedar branches, seal it with gauze, and place it in an incubator ( 25 ℃, RH 90%, L:D=14 h:10 h). 15 last-instar larvae were treated, and 3 repetitions were performed. image 3 It is a picture of the last instar larva of the cedar caterpillar in normal state; Figure 4 It is the infection symptom map after the last instar larvae of the cedar caterpillar are infected with Beauveria...

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Abstract

The invention provides a culture method and related application of a beauveria bassiana (BbJL-01) with strong pathogenicity on final instar larvas of dendrolimus houi lajonquiere. The strain has the advantages of simple culture method, large sporulation quantity and strong pathogenicity on final instar larvas of dendrolimus houi lajonquiere; furthermore, the strain is a biological prevention and control method; compared with chemical agents, the strain has more environmental friendliness; meanwhile, the strain can be repeatedly used, so that cost is reduced, and the strain is easy to popularize.

Description

technical field [0001] The invention belongs to agricultural biotechnology, and in particular relates to a strain of Beauveria bassiana BbJL-01 which has strong pathogenicity to the last instar larvae of cedar caterpillars. Background technique [0002] Cedar caterpillar ( Dendrolimus houi Lajonquiere), also known as Yunnan pine caterpillar, belongs to Lepidoptera (Lasiocampidae). The insect is a widely distributed and highly adaptable leaf-eating pest, which can harm various hosts such as cedar and Yunnan pine. Its larvae have 7 age classes, and the damage time is longer. In areas where the insect breaks out, conifers such as cedar are often damaged in patches. In mild cases, the needles and young shoots are eaten, resulting in incomplete branches and leaves affecting their normal growth and development; The growth of conifer species has a non-negligible impact; in addition, the poisonous hairs on its larvae and cocoons can also cause harm to the human body. [0003] I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12N15/11A01N63/30A01P7/04C12R1/645
Inventor 韩小红梁光红林浩宇卢赐鼎周友军
Owner FUJIAN AGRI & FORESTRY UNIV
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