Magnetic particle chemiluminiscence detection kit for human adrenocorticotrophic hormone and preparation method

Abstract

The invention discloses a magnetic particle chemiluminiscence detection kit for human adrenocorticotrophic hormone and a preparation method. The kit comprises magnetic particles coupled with an adrenocorticotrophic hormone capture antibody, an adrenocorticotrophic hormone detection antibody marked by acridinium ester, an adrenocorticotrophic hormone calibrator, chemiluminiscence pre-excitation solution A, chemiluminiscence excitation solution B and cleaning solution. The kit provided by the invention employs the magnetic separation chemiluminescence technology as a detection means, and is combined with the acridinium ester labeling technology at the same time. The direct chemiluminiscence method established by the invention has the advantages of high sensitivity, strong specificity, accuracy, rapidness, short detection time and higher accuracy and repeatability of detection results, and the kit can be suitable for various luminescence detection instruments.

Description

technical field [0001] The invention belongs to the technical field of immunodetection and analysis, in particular to a human adrenocorticotropic hormone immunomagnetic particle chemiluminescent detection kit and a preparation method. Background technique [0002] Adrenocorticotropic hormone, abbreviated as ACTH, is a polypeptide hormone secreted by the pituitary gland of vertebrates. ACTH contains 39 amino acids with a molecular weight of 4500. It can promote the tissue hyperplasia of the adrenal cortex and the production and secretion of corticosteroids. Its production and secretion are directly regulated by hypothalamic corticotropin releasing factor (CRF). Excess corticosteroids can in turn affect the pituitary and hypothalamus, reducing their activity. It has the effect of stimulating the development and function of the adrenal cortex. Mainly acts on the adrenal cortex zona to stimulate the secretion of glucocorticoids. When excessive corticosteroids are injected i...

AI Technical Summary

Problems solved by technology

However, although the radioimmunoassay method has the advantages of high sensitivity and high specificity, it has many operating steps, expensive reagents, special detection equipment, and makes operators vulnerable to radioactive hazards. At the same time, the service life of the kit It will also be limited by the decay period of the labeled radioactive substance. Therefore, in recent years, many drugs originally determined by RIA have been switched to other immunoassay methods.

Although the enzyme-linked immunosorbent assay is cheap and fast, it is not sensitive enough and is only suitable for the detection and identification of trace substances. CN202033370 U (November 2011) discloses an enzyme-linked immunosorbent assay reagent for human adrenocorticotropic hormone The kit uses a polystyrene reagent plate (PS) as a solid phase carrier, pre-coats a certain concentration of ACTH coupled with an OVA carrier on the microwell strip of the reagent plate, and adds the sample to be tested to form a microtiter plate The coated ACTH competes with the ACTH in the specimen to bind to the anti-ACTH antibody, and then the signal is amplified by avidinated horseradish peroxidase (HRP), and 3,3′,5,5′-tetramethyl-linked Aniline (TMB) and sulfuric acid were used as substrate and stop solution respectively to complete the preparation of human ACTH ELISA kit. The main disadvantage of this method is low sensitivity