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A target colony automatic positioning and identification method

An automatic positioning and identification method technology, applied in the field of biological and medical image processing, can solve the problems of automatic positioning and identification of untargeted colonies, independent colonies and adhesion colonies are not distinguished and discriminated.

Active Publication Date: 2019-06-28
NANJING NORMAL UNIVERSITY +1
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Problems solved by technology

[0004] The present invention provides a method for automatic positioning and identification of target colonies to solve the problem that most of the existing colony identification methods only aim at counting colonies and do not automatically locate and identify target colonies, and do not distinguish between independent colonies and cohesive colonies The problem

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  • A target colony automatic positioning and identification method
  • A target colony automatic positioning and identification method
  • A target colony automatic positioning and identification method

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Embodiment Construction

[0065] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0066] The embodiment of the present invention discloses a method for automatically locating and identifying target colonies. In the embodiment of the present invention, the color image of the culture dish containing the colony is collected by a CCD industrial camera, and the color image of the culture dish containing the colony is processed by the host computer software. For image processing, a liquid crystal display is used for processing and the result shows that the host computer has a main frequency of 2.8Hz and a memory of 8G. The method can be applied to fields such as biological gene extraction.

[0067] refer to figure 1 , is a schematic workflow diagram of a method for automatically locating and identifying target colon...

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Abstract

The invention discloses a method for automatically positioning and identifying a target colony, which comprises the following steps of: acquiring and storing a color image of a culture dish containingthe colony, and carrying out morphological feature acquisition pretreatment on the color image to obtain a contour binary image of the colony and the culture dish; acquiring morphological characteristics of the bacterial colony according to the contour binary image; screening out independent bacterial colonies from the bacterial colonies according to preset screening conditions; calculating to obtain the number, position information and area characteristics of the independent colonies, and identifying target colonies from the independent colonies according to preset identification conditionsand the number and area characteristics of the independent colonies; and displaying and storing the position information and the image of the target colony. By the adoption of the method, independentand adhesion bacterial colonies can be distinguished, a solution is provided for automatic positioning and recognition of the target bacterial colonies, and the method has the advantages of being highin processing speed and recognition accuracy.

Description

technical field [0001] The invention relates to the technical field of biological and medical image processing, in particular to a method for automatically locating and identifying target colonies. Background technique [0002] Colony identification is a basic and important task for food, biology, medical institutions and scientific research institutions to carry out the required colony culture. In the field of food, such as the selection of active yeast before fermentation of yeast powder, and the fermentation of alcohol; in the field of biology, such as the transplantation of new colonies, and the selection of independent or large individual colonies is even more essential. basic work. At home and abroad, we usually look for independent target colonies by visual observation and manually extract them and put them into the culture medium. This method is labor-intensive, time-consuming, inefficient, and cannot save the negatives of each observation stage. In recent years, w...

Claims

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Application Information

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IPC IPC(8): G06K9/00
Inventor 谢非汪璠杨继全刘益剑杨建飞朱莉娅汪壬甲唐文来
Owner NANJING NORMAL UNIVERSITY
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