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A kind of refining process of Ganoderma lucidum polysaccharide

A Ganoderma lucidum polysaccharide and process technology, which is applied in the refining process of Ganoderma lucidum polysaccharide, can solve the problems of high energy consumption in the concentration process of the extract, high energy consumption in the separation and purification process, and incomplete removal of protein impurities, so as to achieve complete removal of protein impurities and concentration High separation efficiency and low loss rate

Active Publication Date: 2021-03-19
HEFEI UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] With the continuous deepening of the modernization process of the Ganoderma lucidum deep processing industry, the traditional extraction, separation and purification process of Ganoderma lucidum polysaccharides can no longer fully meet the needs of development. The development of new processes has become an important link in the modernization of the extraction, separation and purification technology of Ganoderma lucidum polysaccharides. The purification process requires the development of new processes and new technologies due to the problems of high energy consumption, high loss rate of active ingredients, large residues of protein impurities, low purity of polysaccharides, and organic pollution.
The removal of protein impurities in the extract of Ganoderma lucidum polysaccharides and the concentration of extracts are two key steps in the refining process of Ganoderma lucidum polysaccharides. In order to solve the problem of incomplete removal of protein impurities in the existing extraction, separation and purification process of Ganoderma lucidum polysaccharides, the process of concentration of extracts consumes a lot of energy , The problem of high loss rate of polysaccharides, the present invention designs a refining process of Ganoderma lucidum polysaccharides

Method used

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  • A kind of refining process of Ganoderma lucidum polysaccharide
  • A kind of refining process of Ganoderma lucidum polysaccharide
  • A kind of refining process of Ganoderma lucidum polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Dried Ganoderma lucidum fruiting bodies are crushed and passed through a 100-mesh sieve to obtain Ganoderma lucidum fruiting body powder;

[0034] (2) Get 100g of Ganoderma lucidum fruiting body powder and add it to 50 times the quality of water, carry out ultrasonic extraction, centrifuge to get the supernatant, 4756g of polysaccharide crude extract;

[0035] (3) Adjust the pH of the crude polysaccharide extract to 7.8, add trypsin, enzymolyze the protein impurities in the extract at 38°C, centrifuge to get the supernatant, and obtain a polysaccharide enzymolysis solution of 4741g;

[0036] (4) Add cation exchange resin to the polysaccharide enzymolysis solution, stir, remove residual trypsin, filter and get the filtrate, polysaccharide essence extract 4690g;

[0037] (5) The polysaccharide essence extract is treated with a forward osmosis membrane, and the polysaccharide essence extract concentrate is 1000g;

[0038] (6) The concentrated solution of polysaccharid...

Embodiment 2

[0046] (1) Drying and crushing the spores of ganoderma lucidum through a 100-mesh sieve to obtain spore powder of ganoderma lucidum;

[0047] (2) Get 100g of Ganoderma lucidum spore powder and add it to 80 times the quality of water, carry out ultrasonic extraction, centrifuge to get the supernatant, polysaccharide crude extract 7732g;

[0048] (3) Adjust the pH of the crude polysaccharide extract to 7.8, add trypsin, enzymolyze the protein impurities in the extract at 38°C, centrifuge to get the supernatant, and obtain a polysaccharide enzymatic hydrolysis solution of 7713g;

[0049] (4) Add cation exchange resin to the polysaccharide enzymatic hydrolysis solution, stir, remove residual trypsin, filter and get the filtrate, polysaccharide fine extract 7656g;

[0050] (5) The polysaccharide essence extract is treated with a forward osmosis membrane, and the polysaccharide essence extract concentrate is 1000g;

[0051] (6) The concentrated polysaccharide extract was spray-drie...

Embodiment 3

[0059] (1) Dried Ganoderma lucidum fermented mycelia is passed through a 100-mesh sieve after being pulverized to obtain Ganoderma lucidum mycelium powder;

[0060] (2) Get 100g of Ganoderma lucidum mycelium powder and add it to 60 times the mass of water, carry out ultrasonic extraction, centrifuge to get the supernatant, 5741g of polysaccharide crude extract;

[0061] (3) Adjust the pH of the crude polysaccharide extract to 7.8, add trypsin, enzymolyze the protein impurities in the extract at 38°C, centrifuge to get the supernatant, and obtain 5726 g of polysaccharide enzymatic hydrolysis;

[0062] (4) Add cation exchange resin to the polysaccharide enzymolysis solution, stir, remove residual trypsin, filter and get the filtrate, polysaccharide essence extract 5669g;

[0063] (5) The polysaccharide essence extract is treated with a forward osmosis membrane, and the polysaccharide essence extract concentrate is 1000g;

[0064] (6) The concentrated polysaccharide extract was ...

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Abstract

The present invention relates to a refining process of Ganoderma lucidum polysaccharide, which is prepared from dried Ganoderma lucidum by pulverizing and sieving, ultrasonic extracting polysaccharide, trypsin hydrolyzing protein impurities, cation exchange resin deenzyme, forward osmotic membrane treatment, spray drying and other technological steps, so as to obtain refined Ganoderma lucidum polysaccharide. Protein impurities in Ganoderma polysaccharide extract are hydrolyzed with single trypsin, the isoelectric point of trypsin is high than that optimum reaction pH, and after enzymatic hydrolysis, a cation exchange resin is directly used to deenzyme, thus eliminating the need to adjust pH value again, and the process is simple. The deenzymatic polysaccharide extract is filled with 100-fold water-absorbent grafted starch and treated with forward osmotic membrane with molecular weight cut-off of 5000Da. The proteolytic peptides are removed at the same time of concentration. The separation efficiency is high and the energy consumption is low. The trypsin enzymatic hydrolysis, cation exchange resin deenzyme and forward osmosis membrane treatment are carried out in series, which can not only completely remove protein impurities and achieve high purity of polysaccharide, but also mild deproteinization conditions and stable structure of polysaccharide can be achieved.

Description

technical field [0001] The invention belongs to the technical field of extraction, separation and purification of functional components of ganoderma lucidum, and in particular relates to a refining process of ganoderma lucidum polysaccharide. [0002] technical background [0003] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine in my country. It is known as a fairy grass. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have detailed records on the functions of Ganoderma lucidum. Taking Ganoderma lucidum can strengthen the spleen, nourish the stomach, increase vitality, Detoxification and beauty, anti-cancer and anti-tumor, physical fitness, blood pressure regulation, suitable for a variety of people. Ganoderma lucidum has been recorded in the "Pharmacopoeia of the People's Republic of China" as a medicinal material, and polysaccharides are one of its main active ingredients. Studies have shown that Ganoderma lucidum p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 罗建平尚贞子潘利华杨思林张玉英魏春厅
Owner HEFEI UNIV OF TECH