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Application of long-chain non-coding rna LINC00205 in the preparation of reagents for diagnosing ovarian cancer or drugs for treating ovarian cancer

A long-chain non-coding, ovarian cancer technology, applied in the application of long-chain non-coding RNA for tumor suppression, in the field of biomedicine

Active Publication Date: 2020-09-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since DEHP is not covalently linked to plastic polymers, it easily leaches into the environment

Method used

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  • Application of long-chain non-coding rna LINC00205 in the preparation of reagents for diagnosing ovarian cancer or drugs for treating ovarian cancer
  • Application of long-chain non-coding rna LINC00205 in the preparation of reagents for diagnosing ovarian cancer or drugs for treating ovarian cancer
  • Application of long-chain non-coding rna LINC00205 in the preparation of reagents for diagnosing ovarian cancer or drugs for treating ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Effect of Nanomolar Dose of MEHP on the Migration and Invasion Ability of Human Ovarian Cancer Cell Lines

[0034]We first explored the effects of low doses of MEHP (0nM, 20nM, 100nM, 500nM) on the migration and invasion of ovarian cancer cells SKOV3 and A2780 in vitro. We found that using 20nM, 100nM or 500nM concentration gradient MEHP (the primary metabolite of DEHP) to treat SKOV3 and A2780 cells for 24 and 48 hours, the healing speed of the scratch area was significantly increased compared with the control group, and the 500nM concentration was used Changes in healing speed are particularly pronounced when treated. Consistent with the results of the scratch healing experiment, compared with the control group, DEHP treatment did not significantly change the invasion ability of SKOV3 and A2780 cells; while MEHP treatment could significantly increase the invasion ability of SKOV3 and A2780 cells. The above experimental results suggest that MEHP and the hyd...

Embodiment 2

[0035] Example 2 MEHP exposure induces epithelial-mesenchymal transition in ovarian cancer cells

[0036] Previous experimental results showed that low doses of MEHP could significantly increase the invasion ability of ovarian cancer cells and promote the metastasis of ovarian cancer cells. The first step of tumor cell invasion and metastasis is EMT. After stimulating SKOV3 and A2780 cells with MEHP (0nM, 20nM, 100nM and 500nM) for 24 hours, we found that both ovarian cancer cell lines underwent EMT. As shown in Figure 2, we used Western blotting to observe the changes of EMT markers. MEHP exposure down-regulated the protein expression of the epithelial marker ZO-1; The protein expression of Slug was up-regulated, and the change of protein expression was most obvious when the concentration of 500nM was used. In addition, we also used immunofluorescence to observe the expression of the interstitial marker Vimentin protein in ovarian cancer cell lines SKOV3 and A2780 cells, and...

Embodiment 3

[0037] Example 3 Changes in the expression of long-chain non-coding RNA after exposure to MEHP

[0038] We observed the expression of lncRNA after MEHP exposure. lncRNAs with a p-value less than 0.05 were defined as differentially expressed genes (DEGs). In the same way as the analysis method of mRNA, we found that compared with the expression of lncRNA in the control group, the expression of a total of 754 lncRNAs in the MEHP exposure group had statistically significant changes, as shown in Figure 3 volcano map and gene heat map, Among the 754 lncRNAs, the expression levels of 695 lncRNAs were up-regulated, and the expression levels of 59 lncRNAs were down-regulated. Among the 754 lncRNAs with differential expression, we screened 39 confirmed lncRNAs and 715 potential lncRNAs. We selected 39 confirmed lncRNAs for further research to explore the relationship between these differentially expressed lncRNAs and the migration, invasion and EMT of ovarian cancer cell lines. As s...

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Abstract

MEHP treatment significantly increases the migration and invasion ability of tumor cells, and the results show that MEHP can induce epithelial-mesenchymal transition of ovarian cancer cells. SKOV3 cells treated by the MEHP with a concentration of 500nM are subjected to transcription group sequencing by a high-throughput sequencing method, the sequencing result is analyzed, and 39 confirmed long-chain non-coding RNAs are screened out; and in the differentially-expressed 39 lnc RNAs, the results show that linc00205 plays an important role in the ovarian cancer cell migration, invasion ability improvement and epithelial-mesenchymal transition induced by the MEHP.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application field of long-chain non-coding RNA for tumor suppression. Background technique [0002] Environmental endocrine disruptors (Environmental endocrine disruptors, EEDs), also known as endocrine disrupting chemicals (Endocrine disrupting chemicals, EDCs), refers to the production, release, transport, and metabolism of natural hormones in the body that maintain the homeostasis of the body's internal environment and regulate the development process. A class of naturally occurring or polluted exogenous substances that cause serious effects through combination and effects. There are hundreds of environmental chemicals that have been proven or suspected to have endocrine disrupting effects, including phthalates, polychlorinated biphenyls, organochlorine pesticides, alkylphenols, and bisphenols. Usually classified according to the disturbed endocrine organs and tissue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/7088A61P35/00A61P15/00
CPCA61K31/7088A61K45/00A61P15/00A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 吴一华夏大静李宏毅
Owner ZHEJIANG UNIV
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