Co-culture device and co-culture method for bacterium such as anaerobic bacterium and epithelial cells

Abstract

To provide a means for co-culturing a bacterium such as an anaerobic bacterium and epithelial cells, disclosed is a culture system for co-culturing a first cell group consisting of one or more kinds of cells and a cell layer or tissue formed of a second cell group consisting of one or more kinds of cells different from the former cells, said culture system being characterized by comprising: a first culture tank for co-culturing under anaerobic conditions the first cell group consisting of one or more kinds of cells of, for example, an anaerobic bacterium and the cell layer or tissue formed ofthe second cell group consisting of one or more kinds of cells such as epithelial cells; a second culture tank for pooling a liquid culture medium under aerobic conditions; one or more matter exchangestructures that are disposed so as to connect the first culture tank to the second culture tank; and the aforesaid cell layer or tissue that is disposed so as to cover the surface on the first culture tank side of the matter exchange structure(s).

Description

technical field [0001] The present invention relates to a device for co-cultivating bacteria such as anaerobic bacteria and epithelial cells and a method for co-cultivating bacteria such as anaerobic bacteria and epithelial cells using the device, and particularly relates to a method for co-cultivating bacteria such as anaerobic bacteria and epithelial cells in a static anaerobic culture chamber (anaerobicchamber). A culture device for co-cultivating bacteria such as anaerobic bacteria and epithelial cells in an internal state, and a co-cultivation method using the same. Background technique [0002] Intestinal bacteria is a general term for bacteria that live symbiotically in the intestines of humans and animals and are always present. The number and types of intestinal bacteria are very large. For humans, there are more than 100 billion intestinal bacteria per 1g of feces, and there are at least about 100 types of intestinal bacteria. It is said that there are still a larg...

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Problems solved by technology

However, when the monolayer membrane of intestinal epithelial cells or equivalent cells was subjected to anaerobic conditions to reproduce the environment in the intestinal tract, the tight junction between cells in the monolayer membrane produced was destroyed within one day, and the Within 48 hours, the cells die and the monolayer membrane peels off, so it is impossible to culture intestinal bacteria and intestinal epithelial cells in coexistence (Patent Document 1)

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