Method for producing dictyophorarubrovolvata test tube mother strain

A production method and technology of Pseudomonas erythraea are applied in directions such as botanical equipment and methods, horticulture, plant cultivation, etc., and can solve the problems of weak mycelium of P. Problems such as tube transfer or bacterial expansion, to achieve the effects of rapid growth, good absorption and utilization of mycelium, and overcoming technical obstacles

Inactive Publication Date: 2019-08-09
贵州织金毫米农业科技发展有限公司
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AI Technical Summary

Problems solved by technology

[0002] At present, PDA medium is commonly used in domestic edible fungus parent culture medium. PDA medium has the advantages of low production cost, easy to obtain raw materials, and easy preparation. The surface, not the problem of growing inside the medium
When using PDA medium to prepare the parent species of Dictyophora edulis, compared with most other edible fungi, the mycelium of Dictyophora edulis species is extremely weak, and the growth rate is extremely slow, about 0.5-1mm / d, covering It takes 50-60 days on a 10cm slope, and the aerial hyphae begin to turn purple after about 20 days of growth, showing signs of aging, which is not conducive to subsequent tube transfer or strain propagation. Therefore, the current production method of mother species fundamentally limits Hongtuo Industrialized Cultivation of Dictyophora

Method used

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  • Method for producing dictyophorarubrovolvata test tube mother strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation and sterilization of sawdust medium

[0033] The formula is as follows: 70% poplar sawdust, 14.8% wheat bran, 10% corn flour, 3% soybean flour, 1% gypsum, 1% sugar, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate.

[0034] The above components were mixed according to the ratio, the water content of the culture medium was adjusted to 65%, and a 10cm slope was made in the test tube.

[0035] The slanted culture medium was sterilized by high-pressure steam, kept under the condition of 0.13Mpa for 30 minutes after excluding the cold air, and after the sterilization was completed, it was taken out and cooled for later use.

[0036] 2. Three-point inoculation method

[0037]Select 7 mature and healthy fruiting bodies of Dictyophora, sterilize with potassium permanganate solution for 1.5 minutes, cut the fruiting body into two longitudinally with a sterilized inoculation knife in the ultra-clean workbench, and then cut the inner stipe part Divide i...

Embodiment 2

[0041] 1. Preparation and sterilization of sawdust medium

[0042] The formula is as follows: 63.4% of walnut wood chips, 20% of wheat bran, 5% of corn flour, 10% of soybean flour, 0.5% of gypsum, 0.5% of sugar, 0.1% of potassium dihydrogen phosphate, and 0.5% of magnesium sulfate.

[0043] The above components were mixed according to the ratio, the water content of the culture medium was adjusted to 60%, and a 10cm slope was made in the test tube.

[0044] The slanted culture medium was sterilized by high-pressure steam, kept under the condition of 0.11Mpa for 30 minutes after excluding the cold air, and after the sterilization was completed, it was taken out and cooled for later use.

[0045] 2. Three-point inoculation method

[0046] Select 8 mature and healthy fruiting bodies of Dictyophora, sterilize them with sodium dichloroisocyanurate solution for 1 min, and cut the fruiting bodies into two longitudinally with a sterilized inoculation knife on the ultra-clean workbenc...

Embodiment 3

[0050] 1. Preparation and sterilization of sawdust medium

[0051] The formula is as follows: 80% chestnut wood chips, 5% wheat bran, 11.4% corn flour, 1% soybean flour, 1.5% gypsum, 0.5% sugar, 0.5% potassium dihydrogen phosphate, 0.1% magnesium sulfate.

[0052] The above components were mixed according to the ratio, the water content of the culture medium was adjusted to 70%, and a 10cm slope was made in the test tube.

[0053] The slanted culture medium was sterilized by high pressure steam, kept under the condition of 0.12Mpa for 30 minutes after excluding the cold air, and after the sterilization was completed, it was taken out and cooled for later use.

[0054] 2. Three-point inoculation method

[0055] Select 7-8 mature and healthy fruiting bodies of Dictyophora, sterilize them with potassium permanganate solution for 1 min, and cut the fruiting bodies into two longitudinally with a sterilized inoculation knife on the ultra-clean workbench (or inoculation box). Then ...

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Abstract

The invention provides a method for producing a dictyophorarubrovolvata test tube mother strain and belongs to the technical field of edible fungi cultivation. The method for producing the dictyophorarubrovolvata test tube mother strain comprises the following steps that the water content of a wood bit culture medium is adjusted to range from 60% to 70%, sterilization is carried out, and a slant culture medium is prepared, wherein the wood bit culture medium comprises wood bits, wheat bran, corn flour, soybean flour, gypsum, sugar, monopotassium phosphate, magnesium sulfate and water, and thewood bits comprise wood bits obtained by processing wood of broad-leaf deciduous species; a dictyophorarubrovolvata sporocarp tissue block is equally divided into three parts, the culture medium is inoculated with three tissue blocks, the tissue blocks are closely attached to the culture medium, and mother strain culture is carried out. By means of the method for producing the dictyophorarubrovolvata test tube mother strain, the culture time of the dictyophorarubrovolvata test tube mother strain is obviously shortened, and the dictyophorarubrovolvata test tube mother strain can be demised foruse generally 25-30 days later; compared with a traditional mother strain production method, the time is shortened by 20 days, and then the demising infection rate is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a production method of a test-tube parent species of Dictyophora edulis. Background technique [0002] At present, PDA medium is commonly used in domestic edible fungus parent culture medium. PDA medium has the advantages of low production cost, easy to obtain raw materials, and easy preparation. The surface, not the problem of growing inside the medium. When PDA medium is used to prepare the parent species of Dictyophora edulis, compared with most other edible fungi, the mycelium of Dictyophora edulis species is extremely weak, and the growth rate is extremely slow, about 0.5-1mm / d, and the growth rate is very slow. It takes 50-60 days on a 10cm slope, and the aerial hyphae begin to turn purple after about 20 days of growth, showing signs of aging, which is not conducive to subsequent tube transfer or strain propagation. Therefore, the current prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 杨念龙王波刘德宾徐彦军
Owner 贵州织金毫米农业科技发展有限公司
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