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Extraction method and application of mulberry leaf alkaloids

A technology of mulberry leaf alkaloid and extraction method, which is applied in the fields of application, medical preparations containing active ingredients, food science, etc. damage, improvement of liver fibrosis, and improvement of dyslipidemia

Inactive Publication Date: 2019-08-20
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are no studies on the effects of mulberry leaf alkaloids on liver fibrosis

Method used

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  • Extraction method and application of mulberry leaf alkaloids
  • Extraction method and application of mulberry leaf alkaloids
  • Extraction method and application of mulberry leaf alkaloids

Examples

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Embodiment 1

[0031] Mulberry leaf powder, particle size ≤0.2cm; provided by Silkworm Research Institute of Chongqing Academy of Animal Husbandry Sciences.

[0032] Preparation of mulberry leaf alkaloids: Weigh mulberry leaf powder, mix mulberry leaf powder with pure water according to the ratio of material to liquid mass ratio 1:30, 60°C, power 600W, ultrasonic treatment for 60min, and perform ultrasonic extraction; repeat the extraction twice, The filtrates were combined, and the filtrate was concentrated under reduced pressure with a Shanghai Yarong RE-5298A rotary evaporator at 80° C. in a water bath (rotating at 100 rpm) to obtain a 15-fold concentrate.

[0033] Pass the concentrated solution through D101 macroporous resin at a flow rate of 2.1BV / h; then pass the purified solution through D101 through 732 cationic resin at a flow rate of 2.5BV / h for adsorption, first wash with distilled water until neutral, and then use 0.5mol / L ammonia Wash the ammonia water eluate eluted with 2 times...

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Abstract

The invention provides an extraction method and application of mulberry leaf alkaloids, and belongs to the technical field of application of mulberry leaf alkaloids. The extraction method of the mulberry leaf alkaloids comprises the following steps: 1) mixing mulberry leaves with water, carrying out ultrasonic extraction for 1-3 times so as to obtain extracts, and combining the extracts so as to obtain a crude mulberry leaf alkaloid extract; and 2) sequentially performing decompressed concentration, D101 macroporous resin adsorption, cationic resin adsorption and AB-8 macroporous resin adsorption on the crude mulberry leaf alkaloid extract so as to obtain the mulberry leaf alkaloids. In the step 1), the power of the ultrasonic extraction is 500-700W; the temperature of the ultrasonic extraction is 55-65 DEG C; and the time of each ultrasonic extraction is 50-70min. The mulberry leaf alkaloids extracted by the extraction method are high in total alkaloid content, so that, the mulberry leaf alkaloids are capable of significantly reducing levels of markers of liver fibrosis; and the mulberry leaf alkaloids has improvement effects on hepatic fibrosis in mice.

Description

technical field [0001] The invention belongs to the technical field of mulberry leaf alkaloid application, and in particular relates to an extraction method and application of mulberry leaf alkaloid. Background technique [0002] Liver fibrosis is the final result of chronic inflammatory response induced by various stimuli such as viral infection, alcohol, drug toxicity or other factors (Wu J, Zern M A. Hepatic stellate cells: a target for the treatment of liver fibrosis[J]. Journal of gastroenterology,2000,35(9):665-672. DOI:10.1007 / s005350070045.; Gebhardt R.Oxidative stress,plant-derivedantioxidants and liver fibrosis[J].Planta Medica,2002,68(04):289- 296. DOI: 10.1055 / s-2002-26761.). Liver fibrosis is also an intermediate link between liver injury and liver cirrhosis. Delaying or reversing the occurrence of liver fibrosis is the key to curing most chronic liver diseases. [0003] Mulberry leaves are widely distributed throughout the country and have a long history as a...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61P1/16A23L33/105A61K127/00
CPCA23V2002/00A61K36/605A61K2236/331A61K2236/55A23L33/105A61P1/16A23V2200/32
Inventor 黄先智丁晓雯杨敏沈诗桀
Owner SOUTHWEST UNIVERSITY
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