Inhibitor for inhibiting targeting linc01023 gene of human brain glioma and application of inhibitor

A technology for human brain glioma and brain glioma, applied in the direction of genetic engineering, antineoplastic drugs, medical preparations containing active ingredients, etc., to achieve strong pertinence, inhibit cell proliferation, reduce drug resistance and side effects Effect

Inactive Publication Date: 2019-08-23
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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Problems solved by technology

(Yu M, etal.Linc00152promotes malignant progression of gliomastem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway[J].Molecul...

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  • Inhibitor for inhibiting targeting linc01023 gene of human brain glioma and application of inhibitor
  • Inhibitor for inhibiting targeting linc01023 gene of human brain glioma and application of inhibitor
  • Inhibitor for inhibiting targeting linc01023 gene of human brain glioma and application of inhibitor

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Embodiment Construction

[0032] 1. Glioma cell culture and preparation of conditioned medium

[0033] Human glioma cell line U87 was purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. U87 glioma cells were cultured in DMEM medium containing 10% fetal bovine serum. Culture medium was placed at 37°C 5% CO 2 In the incubator in the constant temperature incubator, the culture medium is changed every 2 days, and the cells can grow to a single layer in about 2-3 days.

[0034] 2. Sample collection

[0035] 1) First prepare the cryopreservation tubes used to preserve the tissue, and use a black oily marker to mark the sample number, collection date and other information on the cryopreservation tubes;

[0036] 2) Intraoperative resection of glioma tissue (operated by a neurosurgeon with more than 5 years of operating experience);

[0037] 3) Divide the resected glioma tissue specimens into two parts, send one part to pathology, and put the ...

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Abstract

The invention belongs to the field of biological gene therapy and particularly relates to an inhibitor for inhibiting a targeting linc01023 gene of brain glioma and application of the inhibitor. The nucleotide sequence of the targeting inhibitor is 5'-CTCCTTCTGACGGCGGAAA-3' (SEQ ID No.1). In order to achieve the gene therapy purpose of inhibiting the proliferation, migration and invasion of brainglioma, based on an RNAi technology, the inhibitor can act on the linc01023 gene in a targeted way by artificially synthesizing linc01023-targeted shRNA to promote the degradation of the linc01023 gene, a result of reducing the expression of the linc01023 gene is obtained, and a new thought is provided for clinically treating brain glioma.

Description

technical field [0001] The invention belongs to the field of biological gene therapy, and specifically relates to an inhibitor targeting linc01023 gene for inhibiting human malignant glioma and its application. Background technique [0002] Glioma is the most common primary malignant tumor of the central nervous system, accounting for about 45% of all primary intracranial tumors. Although with the development of science and technology, various treatment methods such as surgery, radiotherapy, chemotherapy, gene therapy, biological therapy, traditional Chinese medicine, and immunotherapy have appeared in the treatment of glioma, the prognosis of patients is still poor. In the past 20 years, the incidence of glioma has gradually increased, but the prognosis of patients has not improved significantly. According to statistics, the median survival period of anaplastic glioma after traditional surgery and postoperative adjuvant standard radiotherapy and chemotherapy is only 2-5 ye...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/713A61P35/00A61P35/04
CPCA61K31/713A61P35/00A61P35/04C12N15/113C12N2310/14
Inventor 于明军于诗嘉陈铎关俊宏
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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