A method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples

Abstract

The invention discloses a method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples. The steps are as follows: using methylamine and triethylamine-isopropanol solution to prepare a reference solution; The alcohol solution was dispersed and centrifuged, and the supernatant was taken to obtain the test solution; triethylamine-isopropanol solution was used as the blank solvent; a gas chromatograph equipped with an FID detector was used as the detection instrument, and the blank solvent and the reference substance were respectively Solution and need testing solution sample injection, carry out gas chromatographic detection, obtain detection result. The present invention adopts the isopropanol derivatization method to make methylamine be derivatized into larger molecules by isopropanol, thereby prolonging the retention time, improving the degree of separation, and improving the sensitivity of FID detection. The form of amine is extracted by free treatment, so as to ensure the accuracy of detection and solve the technical problem that methylamine is difficult to be detected.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples. Background technique [0002] Methylamine is widely used in many production fields, mainly used as medicine (caffeine, ephedrine, etc.), pesticides (carbaryl, dimethoate, chlordimeform, etc.), dyes (alizarin intermediates, anthraquinone intermediates, etc. ), explosives and fuels, surfactants, accelerators, and raw materials for rubber additives, photographic chemicals and solvents. In addition, in the process of nucleic acid synthesis, it is often used in the process of nucleic acid deprotection and purification. [0003] In the production process of medicine and nucleic acid, due to the strong alkalinity of methylamine, it often exists in the form of methylamine salt, which is extremely difficult to volatilize. Therefore, in the production process of the product, methyl...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples, using isopropanol derivatization method, in the derivatization method, methylamine is derivatized into larger molecules by isopropanol, and through three Ethylamine inhibits its salt formation so that it can be detected by the detector, which solves the problem that the sensitivity and accuracy cannot meet the detection requirements

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