Method for detecting methylamine and methylamine salt solvent residuals in nucleic acid solid sample

Abstract

The invention discloses a method for detecting methylamine and methylamine salt solvent residuals in a nucleic acid solid sample, The method includes the following steps: configuring reference solutions by using methylamine and triethylamine-isopropanol solutions; carrying out dispersion centrifugation by using a sample and the triethylamine-isopropanol solution, and taking a supernatant to obtaina tested object solution; using the triethylamine-isopropanol solution as a blank solvent; and using a gas chromatograph provided with an FID detector as a detection instrument, sampling the blank solvent, the referent solvent and the tested object solution, and carrying out gas chromatography detection to obtain a detection result. The method uses an isopropanol derivatization method to make methylamine derivate into larger molecules by isopropanol, thereby prolonging retention time, improving the separation degree and improving the sensitivity of FID detection. The methylamine salts are extracted in a form of methylamine by free treatment by adding triethylamine so as to ensure the accuracy of the detection, which solves the technical problem of difficult detection of the methylamine.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples. Background technique [0002] Methylamine is widely used in many production fields, mainly used as medicine (caffeine, ephedrine, etc.), pesticides (carbaryl, dimethoate, chlordimeform, etc.), dyes (alizarin intermediates, anthraquinone intermediates, etc. ), explosives and fuels, surfactants, accelerators, and raw materials for rubber additives, photographic chemicals and solvents. In addition, in the process of nucleic acid synthesis, it is often used in the process of nucleic acid deprotection and purification. [0003] In the production process of medicine and nucleic acid, due to the strong alkalinity of methylamine, it often exists in the form of methylamine salt, which is extremely difficult to volatilize. Therefore, in the production process of the product, methyl...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for detecting methylamine and methylamine salt solvent residues in nucleic acid solid samples, using isopropanol derivatization method, in the derivatization method, methylamine is derivatized into larger molecules by isopropanol, and through three Ethylamine inhibits its salt formation so that it can be detected by the detector, which solves the problem that the sensitivity and accuracy cannot meet the detection requirements

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