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Fully-closed microfluidic chip and emulsion micro-droplet preparation system

A microfluidic chip and preparation system technology, applied in heating or cooling equipment, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems affecting the accuracy of measurement results, the loss of emulsion droplet movement, and the breakage of emulsion droplets. and other problems, to achieve the effect of being conducive to rapid promotion, avoiding aerosol pollution, and reducing professional requirements

Pending Publication Date: 2019-09-03
长春技特生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When reading the results, it is necessary to take out the emulsion droplets from the 96-well plate. In the above process, the emulsion droplets will be damaged, the movement loss of the emulsion droplets, and even aerosol pollution, resulting in false positives and affecting Accuracy of Measuring Effects

Method used

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Embodiment 1

[0035] The present invention will be further described below in conjunction with accompanying drawing:

[0036] Such as Figure 4 , 5 , 6, 1 is the capacitive touch screen, 2 is the LED indicator light, 3 is the translucent protective cover, 4 is the chip pressure plate, 5 is the overall shell, 6 is the compression pump, 7 is the gas cylinder, 8 is the power supply Module, 9 is the valve array, 10 is the control circuit, 11 is the interface A closely connected with the oil phase inlet, 12 is the interface B closely connected with the sample inlet, 13 is the interface C closely connected with the vent hole, 14 Is gland rotating shaft, 15 is pipeline connection.

[0037] Such as figure 1 , 2 , 3, 16 is the emulsion droplet generation structure area, 17 is the PCR structure area, 18 is the oil phase introduction pipeline, 19 is the oil phase introduction port, 20 is the sample introduction port, 21 is the cavity, 22 is the sealing film , 23 is a droplet collection cavity, 24...

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Abstract

The invention relates to a fully-closed microfluidic chip and an emulsion micro-droplet preparation system, and belongs to the technical field of biochemical equipment. The microfluidic chip comprisesan emulsion micro-droplet generating structure and a PCR structure, wherein the emulsion micro-droplet generating structure and the PCR structure are integrated into a chip unit, the emulsion micro-droplet generating structure is used for producing water-in-oil emulsion micro-droplets from an oil phase and a sample aqueous phase, and the PCR structure is a closed structure. According to the present invention, the two structures of the emulsion micro-droplet generating structure and the PCR structure are integrated into the one unit, such that the integrated design reduces the professional requirements on the operator and easily achieves the rapid promotion of the technology; the possibility of the introduction of pollution in PCR is reduced, and the accuracy of the digital PCR detection is improved; the aerosol contamination can be avoided; the emulsion micro-droplets are protected from being damaged during the PCR; the control instrument can have multiple (such as two) working positions for arranging the microfluidic chip; and the operation is simplified, the time is saved, and the efficiency is increased.

Description

[0001] This application is a divisional application. Its original Chinese national application number is 201811200046.2, and the application date is October 16, 2018. The title of the invention is "a fully enclosed microfluidic chip and emulsion droplet preparation system". technical field [0002] The invention belongs to the technical field of biochemical test detection equipment. Background technique [0003] The digital PCR technology uses the water-in-oil emulsion micro-droplet as the micro-reactor of the PCR reaction for PCR amplification. The biggest feature of digital PCR is that it can form a large number of independent reaction spaces for PCR amplification. Microemulsion droplets formed from oil-water two-phase have been used in high-throughput analysis systems, and their application range has a tendency to expand. For example, emulsion droplets are formed for polymerase chain reaction (PCR) binding, and a single copy of a nucleic acid target molecule is amplified...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/00C12M1/38
CPCB01L3/5027B01L7/52B01L2300/0809
Inventor 褚春旭司远吕洋
Owner 长春技特生物技术有限公司
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