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Application of epigallocatechin gallate in preparing preparations for preventing and/or treating porcine epidemic diarrhea virus

A technology for epigallocatechin and porcine epidemic diarrhea, which is applied in the application field of preparing a virus preparation for preventing and/or treating porcine epidemic diarrhea, can solve the problems such as the absence of porcine epidemic diarrhea, and achieves significant effects, inhibiting Adsorption and reduction of cytopathic effects

Active Publication Date: 2021-07-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no specific drug and therapy for porcine epidemic diarrhea

Method used

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  • Application of epigallocatechin gallate in preparing preparations for preventing and/or treating porcine epidemic diarrhea virus
  • Application of epigallocatechin gallate in preparing preparations for preventing and/or treating porcine epidemic diarrhea virus
  • Application of epigallocatechin gallate in preparing preparations for preventing and/or treating porcine epidemic diarrhea virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Effects of epigallocatechin-3-gallate on cytopathic effects caused by porcine epidemic diarrhea virus

[0040] Dilute Vero cells with nutrient solution containing 8% fetal calf serum and count, 5 × 10 5 Cells at a concentration of 1 / well were plated in a six-well plate and placed in a 5% CO 2 37°C incubator. After the cells grow to a density of 70-80% (about 16h), wash the cells twice with PBS solution, pretreat with PBS or EGCG (20μM, 50μM, 100μM) for 1h, and then use porcine epidemic diarrhea virus HLJBY virus strain (0.1MOI) to infect the cells, change the medium after 1h, infect for 24h and the drug exists all the time, observe the morphological changes and pathological changes of the cells under a microscope, see figure 1 . Depend on figure 1 It can be seen that EGCG can reduce the cytopathy caused by porcine epidemic diarrhea virus.

Embodiment 2

[0042] Effect of epigallocatechin-3-gallate on expression level of porcine epidemic diarrhea virus structural protein N in cells and virus titer in supernatant

[0043]Dilute Vero cells with nutrient solution containing 8% fetal calf serum and count, 5 × 10 5 Cells at a concentration of 1 / well were plated in a six-well plate and placed in a 5% CO 2 37°C incubator. After the cells grow to a density of 70-80% (about 16h), the cells are washed twice with PBS solution, pretreated with PBS or EGCG (50μM) for 1h, and then treated with porcine epidemic diarrhea virus HLJBY strain (0.1MOI ) to infect the cells, change the medium after 1 hour, infect 4 hours, 8 hours, 12 hours, 24 hours, 36 hours and the drug has been present, collect the protein samples of the cells, and use Western blot to analyze the changes in the expression level of porcine epidemic diarrhea virus structural protein N. Cells were treated as above, pretreated with PBS or EGCG (20 μM, 50 μM, 100 μM) for 1 h, and t...

Embodiment 3

[0045] Effects of Epigallocatechin-3-gallate on the Infection Process of Porcine Epidemic Diarrhea Virus

[0046] (1) Effect of epigallocatechin-3-gallate on adsorption of porcine epidemic diarrhea virus (Western blot)

[0047] Dilute Vero cells with nutrient solution containing 8% fetal calf serum and count, 5 × 10 5 Cells at a concentration of 1 / well were plated in a six-well plate and placed in a 5% CO 2 37°C incubator. After the cells grow to 70-80% density (about 16h), wash the cells three times with PBS solution, pretreat with PBS or EGCG (20μM, 50μM, 100μM) for 1h, and then use porcine epidemic diarrhea virus HLJBY strain (0.1MOI) infected cells, placed in a refrigerator at 4°C for 1 hour, washed three times with cold PBS, replaced with 2% DMEM, placed in a 37°C incubator, collected cell protein samples after 24 hours, and detected the expression of viral protein N protein level situation, see image 3 . Depend on image 3 It can be seen that EGCG significantly in...

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Abstract

The invention provides the application of epigallocatechin gallate in the preparation of preparations for preventing and / or treating porcine epidemic diarrhea virus, and relates to the technical field of antiviral applications. Epigallocatechin gallate (EGCG) provided by the present invention can effectively inhibit the adsorption, entry, replication, assembly and release of porcine epidemic diarrhea virus, thereby inhibiting the infection of porcine epidemic diarrhea virus, and EGCG It can also directly kill porcine epidemic diarrhea virus, so it can be used for treating porcine epidemic diarrhea disease.

Description

technical field [0001] The invention relates to the technical field of antiviral applications, in particular to the application of epigallocatechin gallate in the preparation of preparations for preventing and / or treating porcine epidemic diarrhea virus. Background technique [0002] Porcine epidemic diarrhea (PED) is a highly contagious intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus (PEDV), characterized by vomiting, diarrhea and loss of appetite . Pigs of all ages are susceptible to the disease, and the incidence rate of suckling pigs, shelf pigs and fattening pigs is as high as 100%, and it causes 100% death of 10-day-old piglets, and fattening pigs grow slowly and lose weight. In recent years, the disease has become prevalent in many countries around the world, and the fatality rate is extremely high, which has caused huge economic losses to the pig industry. [0003] Porcine epidemic diarrhea virus is a single-stranded positive-sense R...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61P1/12A61P31/14
CPCA61K31/353A61P1/12A61P31/14
Inventor 郇长超徐伟音高清清郭停停高崧
Owner YANGZHOU UNIV
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