Application of baicalin in preparation of medicine for treating Marek's disease of chicken

A technology of chicken Marek's disease and baicalin, which is applied in the fields of medicine and antiviral applications, can solve the problems of enhancement and aggravation of symptoms, and achieve the effects of inhibiting replication, reducing cell lesions, and significant infection effects

Pending Publication Date: 2020-04-24
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, MDV continues to evolve, its virulence gradually increases, and its symptoms gradually intensify, which brings greater challenges to the prevention and treatment of Marek's disease

Method used

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  • Application of baicalin in preparation of medicine for treating Marek's disease of chicken
  • Application of baicalin in preparation of medicine for treating Marek's disease of chicken
  • Application of baicalin in preparation of medicine for treating Marek's disease of chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Effects of baicalin on the replication of chicken Marek's virus in CEFs.

[0026] figure 1 It is the CCK-8 result graph showing the effect of baicalin on the activity of chicken embryo fibroblasts (CEF); select baicalin at a safe concentration of 20 μg / mL and 2 μg / mL to pretreat CEF in a 12-well plate, and inoculate 200 PFU of MDV virus after 2 hours After 4 hours of adsorption in a 37°C incubator, the maintenance solution containing the corresponding concentration of baicalin was changed until the cells were harvested. 96 hours after inoculation, some cells were digested and used for counting viral plaques, and RNA was extracted from some cells for real-time PCR to detect the expression levels of Meq and gB genes. Some cells were lysed with RIPA buffer for western blot assay. The remaining virus infected CEF cells for indirect immunofluorescence detection of virus proliferation in the cells. It was found that, compared with the control group, when the concentration ...

Embodiment 2

[0028] Time dependence of baicalin on chicken Marek's virus inhibition in cells.

[0029] The virus-infected CEF cells were harvested at 24, 48, 72, 96, and 120 hours after virus infection, respectively, and used for virus plaque counting, qRT-PCR and indirect immunofluorescence tests. Real-time PCR results showed that the expression levels of Meq and gB genes in CEFs infected with MDV RB-1B and without drugs showed a significant increasing trend over time, while the expression levels of Meq and gB genes in CEFs infected with MDV RB-1B and added drugs The increase in expression was not obvious ( image 3 Middle A, B). Viral plaque counts and IFA results were also consistent with Real-time PCR results ( image 3 Middle C, D). The results showed that 20μg / mL baicalin had a significant effect on inhibiting MDV replication within 120 hours after infection.

Embodiment 3

[0031] Baicalin directly affects the infectivity of MDV virus

[0032] Suspend 200PFU virus with DMEM maintenance solution containing normal 0.5% serum and maintenance solution containing 20 μg / mL drug respectively, and incubate at 37° C. in a 5% CO2 incubator for 1.5 hours. After centrifugation to remove the supernatant, the cells were resuspended and seeded in CEF in a 12-well plate. After 4 hours of adsorption, fresh 0.5% serum maintenance solution was replaced until the virus-infected cells were harvested after 96 hours. Cells were collected for plaque counting and real-time PCR to detect virus proliferation.

[0033] The results showed that the viral gene expression in the drug treatment group ( Figure 4 Middle A) and virus proliferation ( Figure 4 Middle B, C) were significantly lower than the normal virus suspension group. This result indicated that 20 μg / mL baicalin could directly inhibit the infectivity of MDV.

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Abstract

The invention relates to application of baicalin in preparation of a medicine for treating Marek's disease of chicken. The invention specifically relates to application of baicalin in preparation of amedicine for preventing and / or treating Marek's disease of chicken. The baicalin provided by the invention can effectively inhibit replication of the chicken Marek's disease virus so as to inhibit the infection of chicken Marek's disease virus, and the baicalin can also directly influence infectivity of the chicken Marek's disease virus and then can be used for preventing and / or treating the chicken Marek's disease.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to the field of antiviral application technology, in particular to the application of baicalin in the preparation of medicines for preventing and / or treating chicken Marek's disease. Background technique [0002] Marek's disease (MD) is a highly contagious and contagious malignant lymphoid neoplastic disease caused by the infection of αherpesviridae Marek's disease virus (MDV). 1-day-old chicks showed cytolytic infection 3-6 days after artificial inoculation, and degenerative lesions of lymphoid organs appeared 6-8 days after infection, especially thymus and bursa atrophy. In about 2 weeks, monocyte infiltration can be seen in nerves and other organs, and detoxification begins. Clinical symptoms appear at the earliest around 18 days, usually in 3-4 weeks. It is one of the major diseases that harm the poultry industry worldwide, causing significant economic losses. [0003] Chicken Mar...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/22A01N43/16A01P1/00A23K20/121A23K50/75
CPCA61K31/7048A61P31/22A01N43/16A23K20/121A23K50/75
Inventor 钱琨扬帆秦爱建邵红霞金文杰
Owner YANGZHOU UNIV
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