Method for manufacturing tissue chip of HER-2 FISH for gastric cancer

Abstract

The invention discloses a method for manufacturing a tissue chip of HER-2 FISH for gastric cancer, and the method comprises the steps of selecting experimental materials, manufacturing an experimentaltissue chip, dewaxing, removing cross-linking pretreatment, digesting with proteinase K, observing cell digestion effect, repeating digestion, denaturation, hybridization and the like, and furthest protects the integrity of wax block archives through the material selection step of "firstly dividing an experimental area and then cutting a tissue section"; the tissue section area is small, so thatexperimental reagents can be saved, the workload is reduced, and the working efficiency and the accuracy are improved; a plurality of tissue sections are laid on one slide, so that the subsequent processing steps can be reduced, the working efficiency is further improved, and the filing space of a file is saved; the flow for observing the cell digestion effect has the advantages of few processingsteps and short time, does not need lens oil and a counterstain, reduces the cost, avoids the risk of interference of the lens oil and the counterstain, and does not have the risk of tissue damage orslide falling. The method for manufacturing the tissue chip of HER-2 FISH for gastric cancer can greatly reduce the material cost and the labor cost of the experiment and improve the working efficiency and the accuracy.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for fabricating a tissue chip for gastric cancer HER-2 FISH. Background technique [0002] Herceptin combined with chemotherapy is the first-line targeted therapy for patients with HER-2 gene-positive advanced gastric cancer, which brings survival benefits to patients, reduces the risk of death by 35%, significantly prolongs the survival period, and improves the quality of life. The rapid development of molecular biotechnology has provided new thinking and direction for clinical targeted therapy and judgment of prognosis, and provided a new and effective plan for the treatment of patients. HER-2 FISH detection technology is the gold standard for detection of HER-2 gene amplification, and it provides assistance for the diagnosis and treatment plan and prognosis of patients with advanced gastric cancer. The complete preservation of the wax block is very important...

AI Technical Summary

Problems solved by technology

But the same defect is that it will destroy the integrity of the wax block, especially for microcarcinoma specimens

[0004] Moreover, there is obvious heterogeneity in different types of gastric cancer specimens, and there are obvious differences in the time for proteinase K to digest the nucleus. Simply using the same digestion time limit cannot obtain ideal and accurate diagnostic results.

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