Preparation method of tissue chip for gastric cancer her-2 FISH

Abstract

The invention discloses a method for making a tissue chip for gastric cancer HER-2 FISH, which includes selecting experimental materials, making an experimental tissue chip, dewaxing, removing cross-linking pretreatment, digesting with proteinase K, observing the effect of cell digestion, and repeating digestion and denaturation The integrity of the wax block archives is protected to the greatest extent through the material selection step of "drawing the experimental area first and then cutting the tissue section"; the tissue section area is small, which can save experimental reagents, reduce workload, and improve work efficiency and accuracy. ; Laying multiple tissue sections on one slide can reduce subsequent processing steps, further improve work efficiency, and save archive space; the process of observing the effect of cell digestion has fewer processing steps and shorter time, without immersion oil and counterstaining agent, Reduce costs and avoid the risk of being interfered with by both, and there is no risk of tissue breakage or chip loss. The invention can greatly reduce the material cost and labor cost of experiments, and improve work efficiency and accuracy.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method for fabricating a tissue chip for gastric cancer HER-2 FISH. Background technique [0002] Herceptin combined with chemotherapy is the first-line targeted therapy for patients with HER-2 gene-positive advanced gastric cancer, which brings survival benefits to patients, reduces the risk of death by 35%, significantly prolongs the survival period, and improves the quality of life. The rapid development of molecular biotechnology has provided new thinking and direction for clinical targeted therapy and judgment of prognosis, and provided a new and effective plan for the treatment of patients. HER-2 FISH detection technology is the gold standard for detection of HER-2 gene amplification, and it provides assistance for the diagnosis and treatment plan and prognosis of patients with advanced gastric cancer. The complete preservation of the wax block is very important...

AI Technical Summary

Problems solved by technology

But the same defect is that it will destroy the integrity of the wax block, especially for microcarcinoma specimens

[0004] Moreover, there is obvious heterogeneity in different types of gastric cancer specimens, and there are obvious differences in the time for proteinase K to digest the nucleus. Simply using the same digestion time limit cannot obtain ideal and accurate diagnostic results.

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