The invention discloses a method for making a
tissue chip for gastric
cancer HER-2 FISH, which includes selecting experimental materials, making an experimental
tissue chip, dewaxing, removing cross-linking pretreatment, digesting with
proteinase K, observing the effect of
cell digestion, and repeating
digestion and denaturation The integrity of the
wax block archives is protected to the greatest extent through the
material selection step of "drawing the experimental area first and then
cutting the
tissue section"; the
tissue section area is small, which can save experimental reagents, reduce
workload, and improve work efficiency and accuracy. ; Laying multiple
tissue sections on one slide can reduce subsequent
processing steps, further improve work efficiency, and save archive space; the process of observing the effect of
cell digestion has fewer
processing steps and shorter time, without immersion oil and counterstaining agent, Reduce costs and avoid the risk of being interfered with by both, and there is no risk of tissue breakage or
chip loss. The invention can greatly reduce the material cost and labor cost of experiments, and improve work efficiency and accuracy.