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Application of deuterium depleted water as skin external use preparation and skin external use preparation containing deuterium depleted water

A kind of skin external preparation, deuterium-depleted water technology, applied in the field of skin external preparation

Pending Publication Date: 2019-09-10
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on the above-mentioned literature and patent research, there are no patent applications and literature on the use of deuterium-depleted water in skin external preparations with anti-aging, moisturizing, elastic firming, and regeneration repair effects

Method used

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  • Application of deuterium depleted water as skin external use preparation and skin external use preparation containing deuterium depleted water
  • Application of deuterium depleted water as skin external use preparation and skin external use preparation containing deuterium depleted water
  • Application of deuterium depleted water as skin external use preparation and skin external use preparation containing deuterium depleted water

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Embodiment 1 Establishment of 3D full-thickness skin model

[0153] 1.1 Source of skin cells

[0154] Primary cultures of keratinocytes (NHEK) and fibroblasts (NHDF) were obtained from the discarded skin of two healthy individuals undergoing routine surgery, in compliance with the Declaration of Helsinki (Shanghai Cell and Tissue Bank-SOBC, Shanghai). NHDF mixed skin cells from the face of a 47-year-old woman, breast skin cells from a 45-year-old woman, and legs from a 35-year-old woman. The NHEK for the 3D experiment came from the foreskin of a 16-year-old male donor, and the NHEK culture donor for the 2D experiment was a 43-year-old.

[0155] 1.2 Human skin substitute culture in vitro

[0156] Dermal substitute (DE) preparation, NHDF from an adult donor (42 years old) in 2.5 × 10 5 cells / cm 2 The concentration of seeded on chitosan-collagen-glycosaminoglycan framework material. The dermal substitute (DE) was heated at 37°C, 5% CO 2 21 days under the environment ...

Embodiment 2

[0158] 2.1 Preprocessing

[0159] The 3D full-thickness skin model construction process was divided into a blank control group and a deuterium-depleted water group. The culture medium for each liquid change in the blank control group was prepared from pure water and DMEM powder (purchased from Gibco), and passed through a 0.22 μm filter Filtered and stored at 4°C, from the first medium exchange after NHDF inoculation (the first day) to the end (the 33rd day); the deuterium-depleted water group replaced pure water with deuterium-depleted water with a deuterium content of 50ppm, The rest of the operations were the same as the blank group.

[0160] 2.2 Histological and immunohistological analysis

[0161] After culturing for 33 days, the samples of each group were collected, quickly transferred to neutral buffer solution containing 4% formalin, fixed for 24 hours, embedded in paraffin or frozen in OCT at -20°C. Paraffin-embedded formalin-fixed samples were then cut into 5 μm se...

Embodiment 3

[0175] Change the 50ppm deuterium-depleted water of the preparation medium in embodiment 2 into 132ppm deuterium-depleted water, and all the other operations are the same as in embodiment 2.

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PUM

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Abstract

The invention discloses an application of deuterium depleted water in preparing a skin external use preparation, and the skin external use preparation containing the deuterium depleted water. The invention finds for the first time that the deuterium depleted water having a deuterium content of not more than 132 ppm has the functions of being capable of significantly promoting proliferation of epidermal keratinocyte cells, enhancing expression of cell proliferation-related protein Ki-67, increasing the thickness of dermis / epidermis, improving homeostasis of the epidermis, and the like. The deuterium depleted water is utilized as an anti-aging active component, a moisture retention active component, and a repair active component in the skin external use preparation, therefore, the skin external use preparation containing the deuterium depleted water of the invention has efficacy of increasing skin elasticity, being resistant to wrinkle, preserving moisture, and repairing aged skin.

Description

technical field [0001] The invention relates to the application of deuterium-depleted water as an external preparation for skin and the external preparation for skin containing it. Background technique [0002] Water that exists in nature is generally composed of 2 hydrogen atoms and 1 oxygen atom, but hydrogen atoms have 3 isotopes with different masses, hydrogen (H), deuterium (D, heavy hydrogen) with atomic weights of 1, 2, and 3 respectively ), tritium (triotium). In natural water, the content of heavy hydrogen is about 150ppm, and the water combined with D instead of H is heavy water. Studies at home and abroad have shown that deuterium is harmful to the survival, development and reproduction of living organisms. The scientific community refers to water with deuterium less than 150ppm as deuterium depleted water (DDW). Deuterium-depleted water with low deuterium content is called light hydrogen molecular water, also known as ultra-light water and deuterium-depleted w...

Claims

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Application Information

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IPC IPC(8): A61K8/19A61Q19/08A61Q19/00
CPCA61K8/19A61Q19/08A61Q19/00
Inventor 姜山山摩根多斯桑托斯蒋丹丹李慧
Owner JALA GROUP CORPORATION
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