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A dry cell model and its establishment method and application

A cell model and method-building technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problem of large influence on the stability of experimental results, unstable wind speed of ultra-clean typhoons, and poor uniformity of wind speed, etc. problems, to achieve the effect of high accuracy of result evaluation, easy operation and high reproducibility of results

Active Publication Date: 2021-07-02
BLOOMAGE BIOTECHNOLOGY CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to maintain the temperature accurately in the oven. Generally, the minimum temperature of the oven is the ambient temperature plus 10°C. In the hot summer, the oven temperature is generally 40-45°C. Drying at this temperature will cause a certain degree of thermal damage to the cells.
The wind drying method generally uses the wind of the ultra-clean bench to dry and damage the cells in the ultra-clean bench. The disadvantage of this method is that the ultra-clean bench with vertical airflow is required, and the wind speed of the ultra-clean bench is unstable and uniform. Poor, great impact on the stability of the experimental results

Method used

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  • A dry cell model and its establishment method and application
  • A dry cell model and its establishment method and application
  • A dry cell model and its establishment method and application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0139] Experimental example 1 Desiccator method prepares the dry cell model of human skin fibroblast

[0140] 1.1 Test material

[0141] Experimental materials: human skin fibroblasts, RPMI-1640 medium, fetal bovine serum (FBS), trypsin.

[0142] Main equipment: glass desiccator (diameter 300mm), temperature and hygrometer, inverted microscope, ultra-clean workbench, carbon dioxide incubator.

[0143] 1.2 Experimental method and process

[0144] 1.2.1 Preparation before experiment

[0145] Prepare a glass desiccator and add 500 g of silica gel to dry the particles (4 hours at 120° C. in an oven). Seal it for 1 hour, measure the relative humidity in the desiccator to be 10.0%, the temperature is 25° C., and irradiate for 1 hour under the ultraviolet lamp.

[0146] 1.2.2 Planking

[0147] Take 5×10 4 Cells / mL The recovered human skin fibroblasts of the third generation were inoculated on a 96-well plate, 125 μL per well, and 5 replicate wells were set for each sample. At ...

experiment example 2

[0159] Experimental Example 2 Desiccator Method Preparation of Dry Cell Model of Human Skin Fibroblasts

[0160] 2.1 The specific parameters are the same as in Experimental Example 1, where the drying time in 1.2.3 is replaced with 30 minutes.

[0161] 2.2 Results and analysis

[0162] The experiment was repeated three times, and the cell viability is shown in Table 2 below.

[0163] Table 2 Cell viability (%)

[0164]

[0165] Note: ** means p<0.01 compared with the non-dried treatment group

[0166] Conclusion: It is feasible to use a desiccator to dry and damage cells, and the error of three parallel experiments is within 5.0%. The drying damage effect of Experimental Example 2 is more significant.

experiment example 3

[0167] Experimental example 3 Desiccator method prepares human skin keratinocyte drying model

[0168] 3.1 Experimental materials

[0169] Experimental materials: human skin keratinocytes (HaCaT), DMEM high-glucose medium, fetal bovine serum, trypsin.

[0170] Main equipment: glass desiccator (300mm in diameter), inverted microscope, ultra-clean bench, carbon dioxide incubator, temperature and humidity meter

[0171] 3.2 Experimental method and process

[0172] 3.2.1 Preparation before experiment

[0173] Prepare a glass desiccator and add 500 g of silica gel to dry the particles (4 hours at 120° C. in an oven). Seal it for 1 hour, measure the relative humidity in the desiccator to be 9.0%, the temperature is 26°C, and irradiate for 1 hour under the ultraviolet lamp.

[0174] 3.2.2 Planking

[0175] Take 5×10 5 Seed the human skin keratinocytes of the third passage after resuscitation in a 96-well plate at a density of cells / mL, 100 μL per well, 37 ° C, 5% CO 2 Conditio...

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Abstract

A method for establishing a dry cell model, comprising the following steps: a cell culture step, culturing on a cell culture plate under temperature-controlled and humidity-controlled conditions; a cell drying step, sucking up the cell culture fluid after the cell culture step, Less than 0.1m / s, relative humidity at 10.0% and below, exposing cells at 25°C±5°C; re-culture step, re-cultivate cells under temperature-controlled and humidity-controlled conditions to make the dried cells vigor Reduced to 45%‑70%, where cell viability is measured by the WST‑1 method, the absorbance value of the non-dried treatment group after incubation under given conditions is defined as 100%, under given conditions After carrying out the same incubation to the drying treatment group, measure its absorbance value, obtain the detection result of the absorbance value of the drying treatment group / absorbance value of the non-drying treatment group, and the detection result expressed in percentage; this method can Used to evaluate the dryness protection and / or dryness repair performance of cosmetics and their raw materials.

Description

technical field [0001] The invention relates to a method for evaluating the moisturizing properties of cosmetics and their raw materials, in particular to a method for evaluating the moisturizing properties of cosmetics and their raw materials and their protective and repairing effects on dry damage at the cell level. Background technique [0002] Moisturizing is the basic appeal of skin care, and moisturizing effect is the most basic function of cosmetics. Therefore, cosmetics claiming moisturizing occupy the highest proportion in the entire cosmetic category. Researchers are also constantly introducing new mechanisms of action and new evaluation methods for moisturizing efficacy. The "Guidelines for the Evaluation of Cosmetic Moisturizing Efficacy" is the first domestic industry standard on the evaluation of cosmetic efficacy, which plays a role in regulating the order of the cosmetics market and improving the quality of cosmetics. This method is to measure the water cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N5/0629
Inventor 毛华张晓鸥谢文娟王玉玲陈雯雯范馨仪石艳丽郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD