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Human chorionic gonadotropin semi-quantitative detection test paper and preparation method thereof, human chorionic gonadotropin semi-quantitative detection reagent cup, and application of human chorionic gonadotropin semi-quantitative detection test paper and human chorionic gonadotropin semi-quantitative detection reagent cup

A chorionic gonadotropic, semi-quantitative detection technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high subjectivity of test results, inaccurate results, narrow concentration detection range, etc.

Pending Publication Date: 2019-09-24
NANTONG EGENS BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, the technical problem to be solved by the present invention is to overcome the defects of narrow detection range of hCG detection test paper concentration in the prior art, high subjectivity of detection results, and inaccurate results, thereby providing a kind of hCG detection test paper with wide detection range, accurate detection results and high sensitivity. Human chorionic gonadotropin semi-quantitative detection test paper, reagent cup, preparation method and application thereof

Method used

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  • Human chorionic gonadotropin semi-quantitative detection test paper and preparation method thereof, human chorionic gonadotropin semi-quantitative detection reagent cup, and application of human chorionic gonadotropin semi-quantitative detection test paper and human chorionic gonadotropin semi-quantitative detection reagent cup
  • Human chorionic gonadotropin semi-quantitative detection test paper and preparation method thereof, human chorionic gonadotropin semi-quantitative detection reagent cup, and application of human chorionic gonadotropin semi-quantitative detection test paper and human chorionic gonadotropin semi-quantitative detection reagent cup
  • Human chorionic gonadotropin semi-quantitative detection test paper and preparation method thereof, human chorionic gonadotropin semi-quantitative detection reagent cup, and application of human chorionic gonadotropin semi-quantitative detection test paper and human chorionic gonadotropin semi-quantitative detection reagent cup

Examples

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Comparison scheme
Effect test

Embodiment 1

[0067] This embodiment provides a test paper for semi-quantitative detection of human chorionic gonadotropin, including six test paper strips, namely the first test paper strip 11, the second test paper strip 12, the third test paper strip 13, the fourth test paper strip Test paper strip 14, the fifth test paper strip 15 and the sixth test paper strip 16, the structure of each test paper strip is as follows figure 1 As shown, it includes, respectively, a substrate 1 and a sample loading pad 2, a colloidal gold adsorption pad 3, an antibody-carrying film 4, and a water-absorbing pad 5 that are sequentially adhered to the substrate. Membrane 4 and water-absorbing pad 5 are overlapped sequentially, specifically, antibody-carrying membrane 4 is located below colloidal gold adsorption pad 3 and water-absorbing pad 5, sample loading pad 2 is located above colloidal gold adsorption pad 3, and the length of the overlapping part is 1mm.

[0068] On the antibody carrying membrane 4, a ...

Embodiment 2

[0115] This embodiment provides a test paper for semi-quantitative detection of human chorionic gonadotropin. The difference from Example 1 is that the free β antibody is a horse-derived antibody, and the distance between the detection line 6 and the quality control line 7 is 0.3 cm.

[0116] This example also provides the preparation method of the semi-quantitative detection test paper for human chorionic gonadotropin above, which is different from the preparation method in Example 1 in that the free β antibody used is a horse-derived antibody; the preparation of the antibody-carrying membrane During the process, the concentration of anti-α-HCG monoclonal antibody was 2 mg / ml, the concentration of anti-mouse IgG polyclonal antibody was 1 mg / ml, and the distance between the detection line and the quality control line was 0.3 cm; the blocking treatment solution used included, 0.08Mol buffer, 0.3% sugar by mass, 0.8% blocking protein by mass, 0.03% preservative by mass, the buffer...

Embodiment 3

[0118] This embodiment provides a test paper for semi-quantitative detection of human chorionic gonadotropin. The difference from Example 1 is that the free β antibody is a rabbit-derived antibody, and the distance between the detection line 6 and the quality control line 7 is 1.0 cm.

[0119] This example also provides the preparation method of the above semi-quantitative detection test paper for human chorionic gonadotropin, which is different from the preparation method in Example 1 in that the free β antibody used is a rabbit-derived antibody; the preparation of the antibody-carrying membrane During the process, the concentration of anti-α-HCG monoclonal antibody was 3mg / ml, the concentration of anti-mouse IgG polyclonal antibody was 2mg / ml, and the distance between the detection line and the quality control line was 0.7cm; the blocking treatment solution used included, 0.12Mol buffer, 0.7% sugar by mass, 1.2% blocking protein by mass, 0.07% preservative by mass, the buffe...

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Abstract

The invention provides a human chorionic gonadotropin semi-quantitative detection test paper and a preparation method thereof. The test paper adopts a non-mouse free [beta] antibody to ensure that the concentration detection range is wider; in addition, the test paper is provided with at least three test strips, the concentration in a wider range can be subjected in semi-quantitative determination by determining whether the detection lines of the test strips develop color or not, the subjective determination of the color depth is not needed, and the determined concentration range is more accurate. The invention also provides a reagent cup containing the test paper. The reagent cup is convenient and sanitary to use, and is suitable for on-site, instant and rapid detection. The invention also provides the application of the test paper and the reagent cup in detecting human pregnancy cycle, ectopic pregnancy, abortion and trophoblastic lesion.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a test paper for semi-quantitative detection of human chorionic gonadotropin, a reagent cup and a preparation method and application thereof. Background technique [0002] Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by placental trophoblast cells during pregnancy. It consists of two different subunits, α and β, which are connected by non-covalent bonds. In the process of hormone production, secretion, metabolism, etc., hCG molecules will undergo various changes such as fragmentation and dissociation, and thus exist in various molecular forms in blood and urine. hCG is the only placental hormone that does not increase with the increase in placental weight. The reference value of hCG for pregnant women is recorded in the "National Clinical Testing Procedures". The reference value of hCG is 5-50mIU / ml for 0.2-1 week of pregnancy; 2 weeks, hCG ...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/577G01N33/558G01N33/52G01N33/53
CPCG01N33/76G01N33/577G01N33/558G01N33/52G01N33/5304G01N2333/59G01N33/54306
Inventor 孙一品邵志燕欧卫军
Owner NANTONG EGENS BIOTECH
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